LOCUS       sequence-445606-        8215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpS and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaSK
FEATURES             Location/Qualifiers
     source          1..8215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6473..6491)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6487..6534
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6601..6692
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7557..7596
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7627..8215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8116..8135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagatggga gaattactgt atttatacgg tttaattcca acaaaagaag
     3781 cagcagccat agagccgttt ccatcttata aggggtttga cggagaacat tcactgtacc
     3841 caattgcgtt tgatcaggtg acggctgtag tttctaagct ggatgctgac acctattcag
     3901 aaaaagtgat tcaagaaaaa atggagcagg atatgagctg gctgcaggaa aaagcatttc
     3961 atcatcacga aacggtagcc gctttgtacg aagaatttac gatcattcca ttaaaatttt
     4021 gcaccattta taaaggtgaa gaaagtctgc aggcagctat tgagattaac aaagaaaaga
     4081 tagagaattc actgacgctg cttcaaggaa atgaagagtg gaatgtgaaa atttactgtg
     4141 atgatacaga gcttaaaaaa ggaatcagcg aaacgaatga aagcgtgaaa gcgaaaaaac
     4201 aagaaattag tcacttatca ccaggaagac agttttttga aaagaaaaaa atagatcagc
     4261 tgattgaaaa agaattagag cttcacaaaa acaaagtgtg tgaagagata catgacaagc
     4321 taaaagaatt atcgctttat gactctgtta aaaagaattg gagcaaagac gtaactggcg
     4381 cagctgaaca gatggcgtgg aacagcgtgt ttcttctccc gtctctgcag attactaagt
     4441 tcgtaaacga aatagaagag cttcagcaaa ggcttgaaaa taaaggctgg aagtttgaag
     4501 tgacgggacc atggccgccc tatcatttct cgagctttgc gtaaagtagg aggaacagta
     4561 tggcagtcga acataatatg cagtcaagta cgattgtaga tgtgctcgaa aagattttgg
     4621 ataaaggagt cgttatagcg ggggacatca ccgtaggaat tgcagatgtc gagctattaa
     4681 cgataaagat ccgcttgatt gtggcttcgg ttgataaggc aaaagaaatc ggcatggact
     4741 ggtgggaaaa tgatccgtat ctcagttcaa aaggagccaa taacaaagcg ctcgaagaag
     4801 aaaataaaat gctgcatgag cggttaaaaa cgcttgaaga aaaaatagaa acgaaacgtt
     4861 aaaaactgta cgctacttaa aaaatggagg gatttacaat ggcaactgaa acaaaattag
     4921 ataacacaca ggcagaaaac aaggaaaata aaaatgcgga aaacggttca aaagaaaaga
     4981 acggttcaaa agcaagcaaa acaacaagca gcgggccaat caaacgagcg gtagcaggag
     5041 gcatcatcgg tgcaacgatt ggatatgtat cgactcctga aaatcgaaaa agtctccttg
     5101 accgcattga tacagacgaa ttaaaaagca aagcatctga tttaggaaca aaggtaaaag
     5161 aaaaatcaaa aagcagcgtg gccagcctga aaacatctgc gggaagcttg tttaaaaaag
     5221 ataaagataa atcaaaagat gatgaagaaa acgtaaattc ttctagtagc gaaacagaag
     5281 acgataacgt tcaagagtac gacgagttaa aagaagaaaa tcaaactctt caagatcgct
     5341 tatcacagct tgaagaaaaa atgaacatgc ttgttgagct tagcctcaat aaaaatcaag
     5401 acgaagaagc ggaagataca gattccgacg aagaagagaa cgatgagaac gatgaaaacg
     5461 atgaaaacga gcaggacgat gaaaacgaag aagaaacatc taagccacgt aaaaaggata
     5521 aaaaagaagc tgaggaagaa gaaagtgaaa gtgacgaaga cagcgaggaa gaagaggaag
     5581 attctcgctc aaacaaaaaa aataaaaaag taaaaacaga agaagaagac gaagatgaaa
     5641 gcgaagaaga aaaaaaggaa gcgaaaccaa aaaagtcaac agctaaaaaa tcaaaaaata
     5701 caaaagcaaa gaaaaacacg gacgaagaag atgatgaagc aacatctctt tctagtgaag
     5761 acgatacaac agcctaagac gtaaaggagg aaagaaagac atgagtacag gcccttcttt
     5821 ttcaactaaa gacaatacgc ttgaatactt tgtgaaagct tctaataaac acggcttttc
     5881 acttgatatt tcattaaatg taaacggcgc tgtgatttcc ggtaccatga tttcagcaaa
     5941 agaatacttt gattacttaa gcgaaacgtt tgaagaaggc agtgaagtgg ctcaggcgct
     6001 aagcgaacaa ttctctttag caagcgaagc gagcgaatca aacggagaag cagaagccca
     6061 ttttattcat ttgaaaaata caaagattta ctgtggagac agtaaatcta ctccttctaa
     6121 aggcaaaatc ttttggagag ggaaaatagc agaagtagac gggtttttct taggaaagat
     6181 ttctgatgca aaatcaacga gtaaaaagag ttcataaaaa acggcggggt gattgccccg
     6241 ccgtttttta gtgatgtgat gagatgtgca gcttcttttt tcgatacatg agcggagtca
     6301 tcttcatttc ttttcgaaac tgattaataa aatagcttgt actattaaac cctacttgat
     6361 aggcgacttc cgtcacattt gcttctgctt gctgcagtcc ggattcgcga acgcgtctcg
     6421 agagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     6481 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     6541 ggaggaacta tatccgacta gtagcccgcc taatgagcgg gctttttttt aattccccta
     6601 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6661 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6721 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctcgtga
     6781 aagtaaaaga cgcagaggac caattggggg cacgagtggg atacatagaa ctggacttga
     6841 atagcggtaa aatccttgag agttttcgcc ctgaagagcg ttttccaatg atgagcactt
     6901 tcaaagttct gctatgtgga gcagtattat cccgtgtaga tgcggggcaa gagcaactcg
     6961 gacgacgaat acactattcg cagaatgact tggttgaata ctccccagtg acagaaaagc
     7021 accttacgga cggaatgacg gtaagagaat tatgtagtgc cgccataacg atgagtgata
     7081 acactgcggc gaacttactt ctgacaacca tcggtggacc gaaggaatta accgcttttt
     7141 tgcacaatat gggagaccat gtaactcgcc ttgaccgttg ggaaccagaa ctgaatgaag
     7201 ccataccaaa cgacgagcga gacaccacaa tgcctgcggc aatggcaaca acattacgca
     7261 aactattaac tggcgaacta cttactctgg cttcacggca acaattaata gactggcttg
     7321 aagcggataa agttgcagga ccactactgc gttcggcact tcctgctggc tggtttattg
     7381 ctgataaatc tggggcagga gagcgtggtt cacggggtat cattgccgca cttggaccag
     7441 atggtaagcc ttcccgtatc gtagttatct acacgacggg tagtcaggca actatggacg
     7501 aacgaaatag acagattgct gaaatagggg cttcactgat taagcattgg taaaccgata
     7561 caattaaagg ctccttttgg agcctttttt tttggacgga ccggtagaaa agatcaaagg
     7621 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7681 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7741 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     7801 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7861 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7921 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7981 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8041 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8101 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8161 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaa
//
