LOCUS       sequence-445607-        8224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpS and GvpJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaSJ
FEATURES             Location/Qualifiers
     source          1..8224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6482..6500)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6496..6543
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6610..6701
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7566..7605
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7636..8224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8125..8144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtagaagag aaataacgtg cttcacaaat
     3481 tagtaaccgc acccattaac cttgtagtga aaatcggcga aaaagtacag gaagaagctg
     3541 ataaacagct atatgacctt ccgacgattc agcaaaagct cattcagctt caaatgatgt
     3601 ttgagcttgg tgaaattcca gaagaagcgt ttcaagaaaa agaagatgaa ttgttaatga
     3661 ggtacgaaat tgcgaaacgc agagaaattg aacaatggga agagctaaca caaaaaagaa
     3721 atgaggaatc ctagatggga gaattactgt atttatacgg tttaattcca acaaaagaag
     3781 cagcagccat agagccgttt ccatcttata aggggtttga cggagaacat tcactgtacc
     3841 caattgcgtt tgatcaggtg acggctgtag tttctaagct ggatgctgac acctattcag
     3901 aaaaagtgat tcaagaaaaa atggagcagg atatgagctg gctgcaggaa aaagcatttc
     3961 atcatcacga aacggtagcc gctttgtacg aagaatttac gatcattcca ttaaaatttt
     4021 gcaccattta taaaggtgaa gaaagtctgc aggcagctat tgagattaac aaagaaaaga
     4081 tagagaattc actgacgctg cttcaaggaa atgaagagtg gaatgtgaaa atttactgtg
     4141 atgatacaga gcttaaaaaa ggaatcagcg aaacgaatga aagcgtgaaa gcgaaaaaac
     4201 aagaaattag tcacttatca ccaggaagac agttttttga aaagaaaaaa atagatcagc
     4261 tgattgaaaa agaattagag cttcacaaaa acaaagtgtg tgaagagata catgacaagc
     4321 taaaagaatt atcgctttat gactctgtta aaaagaattg gagcaaagac gtaactggcg
     4381 cagctgaaca gatggcgtgg aacagcgtgt ttcttctccc gtctctgcag attactaagt
     4441 tcgtaaacga aatagaagag cttcagcaaa ggcttgaaaa taaaggctgg aagtttgaag
     4501 tgacgggacc atggccgccc tatcatttct cgagctttgc gtaaaagcgg ttaagcgtga
     4561 cagtccagga ggaattaatg gatgcaaccg gtcagccaag caaatggacg aatccacttg
     4621 gatcctgatc aagctgaaca aggcttagcg cagcttgtga tgacagttat tgagctattg
     4681 aggcaaatcg ttgaacgtca tgccatgagg cgggtggagg gtggaacgtt gacggacgaa
     4741 caaattgaaa acttaggaat tgcactaatg aacttagaag aaaaaatgga cgagttgaaa
     4801 gaggtgttcg gtctggatgc agaagattta aatattgatc ttggaccgct aggcagcctg
     4861 ctttaagcgg tcagctgtac gctacttaaa aaatggaggg atttacaatg gcaactgaaa
     4921 caaaattaga taacacacag gcagaaaaca aggaaaataa aaatgcggaa aacggttcaa
     4981 aagaaaagaa cggttcaaaa gcaagcaaaa caacaagcag cgggccaatc aaacgagcgg
     5041 tagcaggagg catcatcggt gcaacgattg gatatgtatc gactcctgaa aatcgaaaaa
     5101 gtctccttga ccgcattgat acagacgaat taaaaagcaa agcatctgat ttaggaacaa
     5161 aggtaaaaga aaaatcaaaa agcagcgtgg ccagcctgaa aacatctgcg ggaagcttgt
     5221 ttaaaaaaga taaagataaa tcaaaagatg atgaagaaaa cgtaaattct tctagtagcg
     5281 aaacagaaga cgataacgtt caagagtacg acgagttaaa agaagaaaat caaactcttc
     5341 aagatcgctt atcacagctt gaagaaaaaa tgaacatgct tgttgagctt agcctcaata
     5401 aaaatcaaga cgaagaagcg gaagatacag attccgacga agaagagaac gatgagaacg
     5461 atgaaaacga tgaaaacgag caggacgatg aaaacgaaga agaaacatct aagccacgta
     5521 aaaaggataa aaaagaagct gaggaagaag aaagtgaaag tgacgaagac agcgaggaag
     5581 aagaggaaga ttctcgctca aacaaaaaaa ataaaaaagt aaaaacagaa gaagaagacg
     5641 aagatgaaag cgaagaagaa aaaaaggaag cgaaaccaaa aaagtcaaca gctaaaaaat
     5701 caaaaaatac aaaagcaaag aaaaacacgg acgaagaaga tgatgaagca acatctcttt
     5761 ctagtgaaga cgatacaaca gcctaagacg taaaggagga aagaaagaca tgagtacagg
     5821 cccttctttt tcaactaaag acaatacgct tgaatacttt gtgaaagctt ctaataaaca
     5881 cggcttttca cttgatattt cattaaatgt aaacggcgct gtgatttccg gtaccatgat
     5941 ttcagcaaaa gaatactttg attacttaag cgaaacgttt gaagaaggca gtgaagtggc
     6001 tcaggcgcta agcgaacaat tctctttagc aagcgaagcg agcgaatcaa acggagaagc
     6061 agaagcccat tttattcatt tgaaaaatac aaagatttac tgtggagaca gtaaatctac
     6121 tccttctaaa ggcaaaatct tttggagagg gaaaatagca gaagtagacg ggtttttctt
     6181 aggaaagatt tctgatgcaa aatcaacgag taaaaagagt tcataaaaaa cggcggggtg
     6241 attgccccgc cgttttttag tgatgtgatg agatgtgcag cttctttttt cgatacatga
     6301 gcggagtcat cttcatttct tttcgaaact gattaataaa atagcttgta ctattaaacc
     6361 ctacttgata ggcgacttcc gtcacatttg cttctgcttg ctgcagtccg gattcgcgaa
     6421 cgcgtctcga gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     6481 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     6541 ttgctgaaag gaggaactat atccgactag tagcccgcct aatgagcggg ctttttttta
     6601 attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6661 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6721 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6781 cgctcgtgaa agtaaaagac gcagaggacc aattgggggc acgagtggga tacatagaac
     6841 tggacttgaa tagcggtaaa atccttgaga gttttcgccc tgaagagcgt tttccaatga
     6901 tgagcacttt caaagttctg ctatgtggag cagtattatc ccgtgtagat gcggggcaag
     6961 agcaactcgg acgacgaata cactattcgc agaatgactt ggttgaatac tccccagtga
     7021 cagaaaagca ccttacggac ggaatgacgg taagagaatt atgtagtgcc gccataacga
     7081 tgagtgataa cactgcggcg aacttacttc tgacaaccat cggtggaccg aaggaattaa
     7141 ccgctttttt gcacaatatg ggagaccatg taactcgcct tgaccgttgg gaaccagaac
     7201 tgaatgaagc cataccaaac gacgagcgag acaccacaat gcctgcggca atggcaacaa
     7261 cattacgcaa actattaact ggcgaactac ttactctggc ttcacggcaa caattaatag
     7321 actggcttga agcggataaa gttgcaggac cactactgcg ttcggcactt cctgctggct
     7381 ggtttattgc tgataaatct ggggcaggag agcgtggttc acggggtatc attgccgcac
     7441 ttggaccaga tggtaagcct tcccgtatcg tagttatcta cacgacgggt agtcaggcaa
     7501 ctatggacga acgaaataga cagattgctg aaataggggc ttcactgatt aagcattggt
     7561 aaaccgatac aattaaaggc tccttttgga gccttttttt ttggacggac cggtagaaaa
     7621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7741 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8221 gaaa
//
