LOCUS       sequence-445639-        4525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses murine Qa-1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4525)
  TITLE     pET-3a - mb2m
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-21a_Qa-1b
FEATURES             Location/Qualifiers
     source          1..4525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(41..88)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     84..102
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(155..172)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1125..1147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1178..1197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1179..1197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1706..1897
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1999..2138
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2054..2073
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2153..2170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2324..2912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2404..2423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3083..3874,3875..3943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3706..3725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3944..4048)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(4074..4525,1..4))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4211..4232)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4423..4442
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgtcccatt cgccaatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
       61 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
      121 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
      181 cggccgcaag cttttaacga tgattccaca ccattttctg tgcatccaga atatgatgca
      241 gggatcctgt ggagacagtg gatggttaag gaggctccca tctcagggtg aggggctcag
      301 gcagcccctc atggtacaca tggcatgtgt aatactgctc cttcccaaga ggcaccacga
      361 cagctgccca cttctggaag gttccatccc ctgcaggcct ggtctccaca agctccatgt
      421 cctgggtcag ctcctcccca ttcaactgcc aggtcagggt gatgtcagca gggtagaagc
      481 ccagggccca gcacctcagg gtgacttcat cttcagatct agggtgatgg gtcacatgtg
      541 cctttggagg gtctgagcgc tgcagtgtct catttcccag ccgtaggtat ctatggagcc
      601 actccacgca aggaccttgc aggtatgccc tctgttggtg ggcctcatcg actgcctctg
      661 acttgtgctt agagatctgt gaggctatgt cattcgcggt ccaggaacgc aggtcctcgt
      721 tcagggagat gtaatcctgg ccatcgtagg cctcctgaca atacccgcgg agcaggcgcc
      781 catcgggccc cacgtcgcag ccgtacatcc actgcagcgt gtgagattcg tcgttactct
      841 gattgtagta gccgagcagg gtcctcaggt ttactctgaa gttcctcccc atgtccctgg
      901 ctttccaagt ctcccgctcc caatactccg gcccctcctg ctcaatccac cgcgcccgag
      961 gctccatcct cggattttcc gcgtcgctgt cgaagcgcac gaactgcgtg tcgtccacgt
     1021 agccgacaat gatgaaccgg ggctccccga ggccgggccg ggacacggcg gtggtgaaat
     1081 accgcagcga gtgtgggcta ccagtcatgc tagccatatg tatatctcct tcttaaagtt
     1141 aaacaaaatt atttctagag ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt
     1201 tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct
     1261 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
     1321 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
     1381 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
     1441 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc
     1501 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
     1561 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
     1621 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
     1681 aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc
     1741 ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga
     1801 tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg
     1861 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     2041 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattg cggtgtgaaa
     2101 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     2161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2761 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3361 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     3421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     4081 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     4141 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     4201 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     4261 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     4321 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     4381 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     4441 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     4501 cgcgcttaat gcgccgctac agggc
//
