LOCUS       sequence-44565-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0026
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcca tatggcctct ggagccgatt caaaaggtga
      121 tgatttatca acagccattc tcaaacagaa gaaccgaccc aatcggttaa ttgttgatga
      181 agccatcaat gaagataaca gcgtggtgtc cttgtcccag cccaagatgg atgaactgca
      241 gttgttccga ggtgacacgg tgttgctaaa aggaaagaaa agacgggaag ctgtatgcat
      301 tgttctttct gatgacacgt gttctgatga gaagattcga atgaatagag ttgttcggaa
      361 taacctccga gttcgcctag gagatgtcat cagcatccag ccatgccctg atgtaaagta
      421 tggcaaacgt atccacgttc tacccatcga tgacacagtg gaaggcatca ctggcaatct
      481 ctttgaggta taccttaagc cgtacttcct ggaagcttat cggcccatcc gtaaaggaga
      541 tatttttctt gtccggggtg ggatgcgtgc tgtggagttc aaagttgtag agacagatcc
      601 cagcccttac tgtattgttg ctccagacac agtgatccac tgtgaggggg agccaatcaa
      661 acgagaggat gaggaggaat ccttgaatga agtaggctat gatgacgtcg gtggttgcag
      721 gaagcagcta gctcagataa aggagatggt ggagctgcca ctgagacatc ctgcgctctt
      781 caaggcgatt ggtgtaaagc ctcctcgggg aatcttgttg tatgggcctc ctgggacagg
      841 gaagaccctg attgctcgag ctgtgg
//
