LOCUS       sequence-445653-        9351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of sfGFP-TetR-p300HAT fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pETduet+sfGFP-TetR-p300HAT
FEATURES             Location/Qualifiers
     source          1..9351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..35
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        123..141
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    142..166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             181..203
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             216..242
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     CDS             243..263
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             267..980
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(297..325)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     920..940
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     regulatory      2583..2592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        2900..2918
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2919..2943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4439..4483
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4521..4539)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4535..4582
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4619..5074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4706..4725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4916..4937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5189..5980,5981..6049))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5812..5831
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6050..6142)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6223..6811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6712..6731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6965..6982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6997..7136
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7062..7081)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7222..7244
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7238..7429)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8001..9083)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9045..9064
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9084..9161)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
       61 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg atcccgcgaa
      121 attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat
      181 tttgtttaac tttaagaagg agatatacca tgggacatca ccatcatcac caccatcatc
      241 acgaaaacct gtattttcag ggatccatga gtaaaggaga agaacttttc actggagttg
      301 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag
      361 agggtgaagg tgatgcaaca aacggaaaac ttacccttaa atttatttgc actactggaa
      421 aactacctgt tccatggcca acacttgtca ctactttaac ttatggtgtt caatgctttt
      481 caagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      541 atgtacagga aagaactata tctttcaaag atgacgggac ctacaagaca cgtgctgaag
      601 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      661 aagatggaaa cattcttgga cacaaattgg aatacaactt taactcacac aatgtataca
      721 tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattaga cacaacgttg
      781 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      841 ctgtcctttt accagacaac cattacctgt ccacacaatc tgtcctttcg aaagatccca
      901 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
      961 gcatggatga actatacaaa ggcgggtccg gtggaagtgg cgggtccgca cggctgaaca
     1021 gagaatcggt tattgatgcg gcactggaac tgctgaatga gacagggatt gacgggctga
     1081 cgacccgcaa gctggcgcag aagctgggaa tagaacagcc gacactttac tggcatgtga
     1141 aaaataaacg ggcgttactg gatgcgctgg cggtggagat cttggcgcgt catcatgatt
     1201 attcactgcc tgcggcgggg gaatcttggc agtcatttct gcgcaataat gcaatgagtt
     1261 tccgccgggc gctgctgcgt taccgtgacg gggcaaaagt gcacctcggc acccgccctg
     1321 atgaaaaaca gtatgatacg gtggaaaccc agttacgctt tatgacagaa aacggctttt
     1381 cactgcgcga cgggttatat gcgatttcag cggtcagtca ttttaccctt ggtgccgtac
     1441 tggagcagca ggagcatact gccgccctga ccgaccgccc tgcagcaccg gacgaaaacc
     1501 tgccgccgct attgcgggaa gcgctgcaga ttatggacag tgatgatggt gagcaggcct
     1561 ttctgcatgg cctggagagc ctgatccggg ggtttgaggt gcagcttacg gcactgttgc
     1621 aaatagtcgg tggtgataaa cttatcatcc ccttttgcgg aggttcggga gggagcggtg
     1681 gatctaagtt ttctgctaaa aggttgccat ctaccagact tggcaccttt ctagagaatc
     1741 gtgtgaatga ctttctgagg cgacagaatc accctgagtc aggagaggtc actgttagag
     1801 tagttcatgc ttctgacaaa accgtggaag taaaaccagg catgaaagca aggtttgtgg
     1861 acagtggaga gatggcagaa tcctttccat accgaaccaa agccctcttt gcctttgaag
     1921 aaattgatgg tgttgacctg tgcttctttg gcatgcatgt tcaagagtat ggctctgact
     1981 gccctccacc caaccagagg agagtataca tatcttacct cgatagtgtt catttcttcc
     2041 gtcctaaatg cttgaggact gcagtctatc atgaaatcct aattggatat ttagaatatg
     2101 tcaagaaatt aggttacaca acagggcata tttgggcatg tccaccaagt gagggagatg
     2161 attatatctt ccattgccat cctcctgacc agaagatacc caagcccaag cgactgcagg
     2221 aatggtacaa aaaaatgctt gacaaggctg tatcagagcg tattgtccat gactacaagg
     2281 atatttttaa acaagctact gaagatagat taacaagtgc aaaggaattg ccttatttcg
     2341 agggtgattt ctggcccaat gttctggaag aaagcattaa ggaactggaa caggaggaag
     2401 aagagagaaa acgagaggaa aacaccagca atgaaagcac agatgtgacc aagggagaca
     2461 gcaaaaatgc taaaaagaag aataataaga aaaccagcaa aaataagagc agcctgagta
     2521 ggggcaacaa gaagaaaccc gggatgccca atgtatctaa cgacctctca cagaaactat
     2581 atgccaccat ggagaagcat aaagaggtct tctttgtgat ccgcctcatt gctggccctg
     2641 ctgccaactc cctgcctccc attgttgatc ctgatcctct catcccctgc gatctgatgg
     2701 atggtcggga tgcgtttctc acgctggcaa gggacaagca cctggagttc tcttcactcc
     2761 gaagagccca gtggtccacc atgtgcatgc tggtggagct gcacacgcag agccaggacg
     2821 gctcgagctg ataagcggcc gcataatgct taagtcgaac agaaagtaat cgtattgtac
     2881 acggccgcat aatcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     2941 tccccatctt agtatattag ttaagtataa gaaggagata tacatagttc atatggaccc
     3001 cttggcagtg tcagcagctt cagtagtgtc aatgtccaat gacgttttga aaccagagac
     3061 gcccaagggg ccaatcataa tcagtaaaaa cccatcaaat ggtattttct atggtccctc
     3121 cttcactaaa cgagagtctc tcaatgctcg aatgtttctg aaatactatg gtgcacacaa
     3181 atttttagac acttacctcc ccgaggattt gaactcgtta tacatttact atcttatcaa
     3241 gttgctaggc tttgaagtta aagatcaagc gcttatcggc accatcaaca gtattgtcca
     3301 tatcaactcg caagagcgtg ttcaagattt gggaagtgca atatctgtca caaatgttga
     3361 agacccattg gcaaaaaagc aaacagttcg tctaatcaaa gatttgcaaa gagcaattaa
     3421 caaagttcta tgtacaagat taagattatc caattttttc actattgatc attttattcg
     3481 aaaattacat accgctagaa aaattttggt cctgactggt gcaggtgttt caacttcatt
     3541 aggcatcccg gacttcagat cttctgaggg gttctattca aagatcaaac atttggggct
     3601 cgatgatccc caagacgttt tcaattacaa tatatttatg cacgacccct ctgttttcta
     3661 taatattgcc aatatggttt tacctccaga aaaaatttat tctccattgc atagtttcat
     3721 taagatgcta caaatgaaag ggaaattatt gagaaattat actcaaaaca ttgataattt
     3781 ggaatcttat gcgggaataa gcacagataa actggtgcag tgccatggct cttttgctac
     3841 tgccacctgc gttacctgcc attggaacct acccggtgag aggatattta ataaaattag
     3901 aaacctcgaa cttccactat gcccgtactg ttacaaaaaa agaagagaat atttcccaga
     3961 gggatataat aataaagtag gtgttgctgc atcacagggt tcaatgtcgg aaaggcctcc
     4021 atatatcctt aactcatatg gcgttctcaa accagatatc acattctttg gcgaagcact
     4081 gccaaataaa tttcataaga gcattcgcga agatatctta gaatgtgatt tgttgatttg
     4141 cattgggaca agtttaaaag tagcgccagt gtctgaaatc gtaaacatgg ttccttccca
     4201 cgttccccaa gtcctgatta atcgtgatcc cgtcaagcac gcagaatttg atttatctct
     4261 tttggggtac tgtgatgaca ttgcagctat ggtagcccaa aaatgtggct ggacgattcc
     4321 gcataagaaa tggaacgatt tgaagaacaa gaactttaaa tgccaagaga aggataaggg
     4381 cgtgtatgtc gttacatcag atgaacatcc caaaaccctc taatgactcg agtctggtaa
     4441 agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta gcgcagctta
     4501 attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     4561 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
     4621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5041 gcgaatttta acaaaatatt aacgtttaca atttctggcg gcacgatggc atgagattat
     5101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5281 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6061 ttcaatcatg attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6121 tgtatttaga aaaataaaca aataggtcat gaccaaaatc ccttaacgtg agttttcgtt
     6181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6361 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6841 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6901 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6961 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     7021 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc
     7081 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     7141 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     7201 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     7261 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     7321 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     7381 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     7441 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     7501 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     7561 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     7621 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     7681 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     7741 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     7801 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     7861 tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg aaggagctga
     7921 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     7981 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     8101 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
     8161 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
     8221 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag
     8281 atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
     8341 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
     8401 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
     8461 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
     8521 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
     8581 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
     8641 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     8701 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
     8761 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
     8821 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
     8881 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
     8941 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
     9001 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
     9061 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
     9121 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
     9181 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     9241 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     9301 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag c
//
