LOCUS       sequence-44566-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   DeLaBarre B, Christianson JC, Kopito RR, Brunger AT
  TITLE     Central pore residues mediate the p97/VCP activity required for 
            ERAD.
  JOURNAL   Mol Cell. 2006 May 19. 22(4):451-62.
  PUBMED    16713576
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 May 19. 22(4):451-62."
COMMENT     Sequence Label: pBD-0011
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggcctct ggagccgatt caaaaggtga tgatttatca
      121 acagccattc tcaaacagaa gaaccgaccc aatcggttaa ttgttgatga agccatcaat
      181 gaagataaca gcgtggtgtc cttgtcccag cccaagatgg atgaactgca gttgttccga
      241 ggtgacacgg tgttgctaaa aggaaagaaa agacgggaag ctgtatgcat tgttctttct
      301 gatgacacgt gttctgatga gaagattcga atgaatagag ttgttcggaa taacctccga
      361 gttcgcctag gagatgtcat cagcatccag ccatgccctg atgtaaagta tggcaaacgt
      421 atccacgttc tacccatcga tgacacagtg gaaggcatca ctggcaatct ctttgaggta
      481 taccttaagc cgtacttcct ggaagcttat cggcccatcc gtaaaggaga tatttttctt
      541 gtccggggtg ggatgcgtgc tgtggagttc aaagttgtag agacagatcc cagcccttac
      601 tgtattgttg ctccagacac agtgatccac tgtgaggggg agccaatcaa acgagaggat
      661 gaggaggaat ccttgaatga agtaggctat gatgacgtcg gtggttgcag gaagcagcta
      721 gctcagataa aggagatggt ggagctgcca ctgagacatc ctgcgctctt caaggcgatt
      781 ggtgtaaagc ctcctcgggg aatcttgttg tatgggcctc ctgggacagg gaagaccctg
      841 attgctcga
//
