LOCUS       sequence-445699-        7966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tc1-1_Xt Transposon with 5 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pQLL-Tc1-1_Xt-5 kb
FEATURES             Location/Qualifiers
     source          1..7966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(793..816)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(840..2018)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          849..1787
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(872..892)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    2064..2288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2277..2294
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2316..3029,3030..3032,3033..3035))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2319..3029,3030..3032,3033..3035))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2361..2382)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2710..2729
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2969..2990
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3036..3092)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3099..3698)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3179..3199)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      3695..3704
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3718..4217)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3766..3785)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4159..4182
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(4683..4700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4683..4699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4709..4727)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      4762..4791
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4813..5400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5301..5320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5412..5439)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5463..5654)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(5655..6446,6447..6515))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAELGPDGKPSRIVVIYTTGSQATMDEQNRQIAEIGAS
                     LIKHW"
     primer_bind     6278..6297
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6516..6618)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6619..6650)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     6702..6719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6731..6747
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6731..6747
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7135..7216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7172..7191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 actatgccaa atcatacagt aataatcggc ctcgtcctcg gacctgagac ctgagatgag
       61 cagcacaccc gcattggcgc ttgcgtcttt agatccacta aaccttgatg gaactccaga
      121 tccctgctgc ttatctgagt cgctcttgta attgagaagg tattggggag gagaacctgg
      181 tttttgctga taccagtaga tcctgtaagg ccctacattg attcctgacc tcagtgtaca
      241 cgtcaaagaa gcggaggcac cggggcttgc actgagggag gatgattgcg tgagaacagg
      301 ttggctaccc ccccctccgg acccgccccc accggaacct ccaccgcctg agcccaggat
      361 cccactggac acggtgacgg ttgtaccttg tccccaaacg tccataccat agtagtaagt
      421 catcataccc cgcgcacaat agtaaacagc tgtgtcttcg ggagttacac tattaagctg
      481 aagagagaac tggtttttgg aagtatctgg atttatagac atcctgcttt tcacacttac
      541 agcataatca ttataccatt tactgcggta gtatgttcta ccgagccatt ccaaccctcg
      601 actgggggac tgcctgatcc aattccatgt cgcggagtta ctactcactg aatcacctga
      661 tatggcacag gtaagactaa gagtctggga aggagtgacc agaccggggc cagattgttg
      721 gagttgcact tgcggcctgg cggcgtggag cagcaaggcc agcggcagga gcaaggcggt
      781 cactggtaag gccttatcgt catcgtcttt gtaatccatg gtggcgaccg gtggatccct
      841 cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac
      901 caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc
      961 ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
     1021 caacctaaag acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc
     1081 cggtactcag tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt
     1141 gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca
     1201 gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt
     1261 gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcga
     1321 gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa
     1381 catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca
     1441 gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca
     1501 ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg
     1561 tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga
     1621 ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc
     1681 tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca
     1741 taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg
     1801 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     1861 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     1921 caccttctct aggcaccggg tcaattgccg acccctcccc ccaacttctc ggggactgtg
     1981 ggcgatgtgc gctctgccca ctgacgggca ccggagcctc acgttaatta accaaggtac
     2041 ccattggcgg ccgccctcag aagccataga gcccaccgca tccccagcat gcctgctatt
     2101 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
     2161 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
     2221 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
     2281 aggcacagtc gaggctgatc agcgagctta attaattact tgtacagctc gtccatgccg
     2341 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     2401 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
     2461 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
     2521 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
     2581 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     2641 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     2701 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     2761 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
     2821 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
     2881 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     2941 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     3001 accccggtga acagctcctc gcccttgctc accattggcc cgggattctc ttcgacatcc
     3061 cctgcttgtt tcaacaggga gaagttagtg gcatcgatgg caccgggctt gcgggtcatg
     3121 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
     3181 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
     3241 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
     3301 ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
     3361 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
     3421 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
     3481 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
     3541 agttcttgca gctcggtgac ccgctcgatg tggcggtccg ggtcgacggt gtggcgcgtg
     3601 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg
     3661 gtggcgaggc gcaccgtggg cttgtactcg gtccccatgg tggcgttggc tgcaggtcga
     3721 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     3781 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     3841 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     3901 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     3961 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
     4021 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
     4081 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
     4141 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
     4201 aagcgcctcc cctacccggt agaattgcta gcaccggtga tgtctagctt ttgaggtgct
     4261 tttggtctac ttctctgtgt caggtagctc ctatttaagt gatttcttga ttgaaacagg
     4321 tgtggcagta atcaggcctg ggggtgacta cagaaattga tattgaaatt gaaaattgaa
     4381 atgtgataaa ccacagttaa gttatttttt aacaaggggg gcaatcactt tttcacacag
     4441 ggccatgtag atttggagtt ttttttctcc cttaataacg taaaccttca tttaaaaact
     4501 gcattttgtg ttcaattatg ttatctttga ctaatagtta acggtttttg atgagcagaa
     4561 acatttaagt gtgacaaaca tgcaaaagaa taagaaatca ggaagggggc aaatagtttt
     4621 tcacaccact gtaaagctta gagacgcaag acactgcgga tcgagacgca ttgcgtggtt
     4681 cgactggccg tcgttttaca acgaaagggc ctcgtgatac gcctattgac tccagcgtaa
     4741 ctggactgca atcaactcac tggctcacct tcacgggtgg gcctttcttc ggtagaaaat
     4801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgag
     4921 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4981 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5041 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5101 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5161 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5221 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5281 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5341 cacctctgac ttgagcatcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5401 aacgccagca acgcagaaag gcccacccga aggtgagcca ggtgattaca tttgggccct
     5461 catcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     5521 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     5581 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     5641 tcctgtttgg tcacttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5701 ttttgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat gcgggagggc
     5761 ttaccatctg gccccagttc tgcaatgata ccgcgggacc cacgctcacc ggctccagat
     5821 ttatcagcaa taaaccagcc agccgggagg gccgagcgca gaagtgatcc tgcaacttta
     5881 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5941 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6001 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6061 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgccagaag taagttggcc
     6121 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6181 gtgagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6241 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6301 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gtaaactctc aaggatctta
     6361 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6421 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6481 ggaataaggg cgacacggaa atgttgaata ctcattttag cttccttagc tcctgaaaat
     6541 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     6601 cctcttacgt gccgatcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg
     6661 gaggtcaggt atgatttaaa tggtcagtaa cgaccgagcg cagcgagtca gtgagcgagg
     6721 aagcggcaca caggaaacag ctatgaccgt ctcggttggc agtgactccg tctctgggcc
     6781 ctacagtggt gtgaaaaact atttgccccc ttcctgattt cttattcttt tgcatgtttg
     6841 tcacacaaaa tgtttctgat catcaaacac atttaactat tagtcaaaga taacacaagt
     6901 aaacacaaaa tgcagttttt aaatgagggt ttttattatt tagggagaaa aaaaatccaa
     6961 acctacatgg ccctgtgtga aaaagtaatt gccccctgaa cctcgagggc caggaagctt
     7021 ccctttggtc tccccctctt aagtaccaca tttgtagagg ttttacttgc tttaaaaaac
     7081 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     7141 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7201 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcaatcgatg cgagggggca
     7261 gggcctgcat gtgaagggcg tcgtaggtgt ccttggtggc tgtactgaga ccctggtaaa
     7321 ggccatcgtg ccccttgccc ctccggcgct cgcctttcat cccaatctca ctgtaggcct
     7381 ccgccatctt atctttctgc agttcattgt acaggccttc ctgagggttc ttccttctcg
     7441 gctttccccc catctcaggg tcccggccac gtctcttgtc caaaacatcg tactcctctc
     7501 ttcgtcctag attgagctcg ttatagagct ggttctggcc ctgctggtac gcgggggcgt
     7561 ctgcgctcct gctgaacttc actctggagc gataggctgc gaagtcgcgt ggtggggcat
     7621 agggctggta atgcttgcgg gtgggcccgg ggcggcgggg agtcatgttc atgtagtcac
     7681 tgtgcaggag cctgctcctc ttactcctca cccagaaaat aataaaggcc actgttacta
     7741 gcaagctata gcaagccagg actcccccaa ccaccaccag cacccaaaaa tcacaggcga
     7801 agtccagccc cctcgtgtgc actgcgcccc ccgccgctgg ccggcacgcc tctgggcgca
     7861 gggacagggg ctgcgacgcg atggtgggcg ccggtgttgg tggtcgcggc gctggcgtcg
     7921 tggtcagcac tgtcagttgc gtgcctccac cgaaaacagc ggctga
//
