LOCUS       sequence-445700-       12685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tc1-1_PM Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 12685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-Tc1-1_PM-10 kb
FEATURES             Location/Qualifiers
     source          1..12685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    32..113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(69..88)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1656..1679)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1679..1688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1703..2881)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1712..2650
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1735..1755)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    2927..3151
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3140..3157
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(3179..3892,3893..3895,3896..3898))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3182..3892,3893..3895,3896..3898))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3224..3245)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3573..3592
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3832..3853
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3899..3955)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3962..4561)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4042..4062)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      4558..4567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4581..5080)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4629..4648)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5022..5045
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(5521..5538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5521..5537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5547..5565)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5633..5737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5738..5806,5807..6598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5956..5975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6769..7357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7258..7277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7511..7528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7540..7556
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7540..7556
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7848..8242
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
       61 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      121 ttgtggtttg tccaaactca atcgatgcga gggggcaggg cctgcatgtg aagggcgtcg
      181 taggtgtcct tggtggctgt actgagaccc tggtaaaggc catcgtgccc cttgcccctc
      241 cggcgctcgc ctttcatccc aatctcactg taggcctccg ccatcttatc tttctgcagt
      301 tcattgtaca ggccttcctg agggttcttc cttctcggct ttccccccat ctcagggtcc
      361 cggccacgtc tcttgtccaa aacatcgtac tcctctcttc gtcctagatt gagctcgtta
      421 tagagctggt tctggccctg ctggtacgcg ggggcgtctg cgctcctgct gaacttcact
      481 ctggagcgat aggctgcgaa gtcgcgtggt ggggcatagg gctggtaatg cttgcgggtg
      541 ggcccggggc ggcggggagt catgttcatg tagtcactgt gcaggagcct gctcctctta
      601 ctcctcaccc agaaaataat aaaggccact gttactagca agctatagca agccaggact
      661 cccccaacca ccaccagcac ccaaaaatca caggcgaagt ccagccccct cgtgtgcact
      721 gcgccccccg ccgctggccg gcacgcctct gggcgcaggg acaggggctg cgacgcgatg
      781 gtgggcgccg gtgttggtgg tcgcggcgct ggcgtcgtgg tcagcactgt cagttgcgtg
      841 cctccaccga aaacagcggc tgaactatgc caaatcatac agtaataatc ggcctcgtcc
      901 tcggacctga gacctgagat gagcagcaca cccgcattgg cgcttgcgtc tttagatcca
      961 ctaaaccttg atggaactcc agatccctgc tgcttatctg agtcgctctt gtaattgaga
     1021 aggtattggg gaggagaacc tggtttttgc tgataccagt agatcctgta aggccctaca
     1081 ttgattcctg acctcagtgt acacgtcaaa gaagcggagg caccggggct tgcactgagg
     1141 gaggatgatt gcgtgagaac aggttggcta cccccccctc cggacccgcc cccaccggaa
     1201 cctccaccgc ctgagcccag gatcccactg gacacggtga cggttgtacc ttgtccccaa
     1261 acgtccatac catagtagta agtcatcata ccccgcgcac aatagtaaac agctgtgtct
     1321 tcgggagtta cactattaag ctgaagagag aactggtttt tggaagtatc tggatttata
     1381 gacatcctgc ttttcacact tacagcataa tcattatacc atttactgcg gtagtatgtt
     1441 ctaccgagcc attccaaccc tcgactgggg gactgcctga tccaattcca tgtcgcggag
     1501 ttactactca ctgaatcacc tgatatggca caggtaagac taagagtctg ggaaggagtg
     1561 accagaccgg ggccagattg ttggagttgc acttgcggcc tggcggcgtg gagcagcaag
     1621 gccagcggca ggagcaaggc ggtcactggt aaggccttat cgtcatcgtc tttgtaatcc
     1681 atggtggcga ccggtggatc cctcacgaca cctgaaatgg aagaaaaaaa ctttgaacca
     1741 ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga
     1801 attacatcaa gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact
     1861 ccatcgcata aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact
     1921 aatcgaggtg cctggacggc gcccggtact cagtggagtc acatgaagcg acggctgagg
     1981 acggaaaggc ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg
     2041 tcctccattt tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa
     2101 ctggtgccga ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgaggc
     2161 caggcaccag agcaggccgg cgagcttgag actacccccg tccgattctc ggtggccgcg
     2221 ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg
     2281 cggccccaaa aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt
     2341 gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga
     2401 aagcagcgag acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag
     2461 gccaggcctc aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct
     2521 cccacccact tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag
     2581 taattcaagg cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca
     2641 cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta
     2701 ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat
     2761 cactttccca gtttaccccg cgccaccttc tctaggcacc gggtcaattg ccgacccctc
     2821 cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc
     2881 ctcacgttaa ttaaccaagg tacccattgg cggccgccct cagaagccat agagcccacc
     2941 gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
     3001 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
     3061 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
     3121 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgagc ttaattaatt
     3181 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     3241 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     3301 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     3361 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     3421 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     3481 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     3541 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     3601 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     3661 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     3721 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     3781 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     3841 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccattg
     3901 gcccgggatt ctcttcgaca tcccctgctt gtttcaacag ggagaagtta gtggcatcga
     3961 tggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg
     4021 cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca
     4081 ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc
     4141 ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct
     4201 ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac
     4261 cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct
     4321 ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt
     4381 cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt
     4441 ccgggtcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta
     4501 cggcccgggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcccca
     4561 tggtggcgtt ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca
     4621 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc
     4681 ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc
     4741 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat
     4801 tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac
     4861 gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa
     4921 ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa
     4981 tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg
     5041 catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagaattg ctagcaccgg
     5101 tggtggagag tttggaatct gattgattga ttgcttctgt ggacaggtgt cttttataca
     5161 ggtaacaagc tgagattagg agcactccct ttaagagtgt gctcctaatc tcagctcgtt
     5221 acctgtataa aagacacctg ggagccagaa atctttctga ttgagagggg gtcaaatact
     5281 tatttccctc attaaaatgc aaatcaattt ataacatttt tgacatgcgt ttttctggat
     5341 ttttgttgtt gttattctgt ctctcactgt tcaaataaac ctaccattaa aattatagac
     5401 tgatcatttc tttgtcagtg ggcaaacgta caaaatcagc aggggatcaa atactttttt
     5461 ccctcactgt aaagcttaga gacgcaagac actgcggatc gagacgcatt gcgtggttcg
     5521 actggccgtc gttttacaac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5581 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5641 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5701 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5761 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5821 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5881 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5941 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6001 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6061 aaagcatctt acggatggca tgacagtaag agaattatgt agtgctgcca taaccatgag
     6121 tgataacacg gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6181 ttttttgcgc aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6241 tgaagccata ccaaacgacg agcgtgatac cacgatgcct gtagcgatgg caacaacgtt
     6301 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6361 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6421 tattgctgat aaatctggag ccggtgagcg tgggtcccgc ggtattattg cagcactggg
     6481 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6541 ggatgaacga aagagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6601 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6661 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6721 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6781 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6841 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6901 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6961 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7021 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7081 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7141 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7201 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7261 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7321 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7381 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7441 ttctgtgggt aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7501 gaccgagcgc agcgagtcag tgagcgagga agcggcacac aggaaacagc tatgaccgtc
     7561 tcggttggca gtgactccgt ctctgggccc tacagtgagg gaaaaaagta tttgatcccc
     7621 tgctgatttt gtacgtttgc ccactgacaa agaaatgatc agtctataat tttaatggta
     7681 ggtttatttg aacagtgaga gacagaataa caacaacaaa atccagaaaa acgcatgtca
     7741 aaaatgttat aaattgattt gcattttaat gagggaaata agtatttgac ccctctgcaa
     7801 aacatgacct cgagggccca tgaggacagc taaaacaata agtaatgtaa aatacagcat
     7861 agcaaaactt taacctccaa atcaagcctc tacttgaatc cttttctgag ggatgaataa
     7921 ggcataggca tcaggggctg ttgccaatgt gcattagctg tttgcagcct caccttcttt
     7981 catggagttt aagatatagt gtattttccc aaggtttgaa ctagctcttc atttctttat
     8041 gttttaaatg cactgacctc ccacattccc tttttagtaa aatattcaga aataatttaa
     8101 atacatcatt gcaatgaaaa taaatgtttt ttattaggca gaatccagat gctcaaggcc
     8161 cttcataata tcccccagtt tagtagttgg acttagggaa caaaggaacc tttaatagaa
     8221 attggacagc aagaaagcga gcttagtgat acttgtgggc cagggcatta gccacaccag
     8281 ccaccacttt ctgataggca gcctgcactg gtggggtgaa ttctttgcca aagtgatggg
     8341 ccagcacaca gaccagcacg ttgcccagga gctgtgggag gaagataaga ggtatgaaca
     8401 tgattagcaa aagggcctag cttggactca gaataatcca gccttatccc aaccataaaa
     8461 taaaagcaga atggtagctg gattgtagct gctattagca atatgaaacc tcttacatca
     8521 gttacaattt atatgcagaa atatttatat gcagaaatat tgctattgcc ttaacccaga
     8581 aattatcact gttattcttt agaatggtgc aaagaggcat gatacattgt atcattattg
     8641 ccctgaaaga aagagattag ggaaagtatt agaaataaga taaacaaaaa agtatattaa
     8701 aagaagaaag cattttttaa aattacaaat gcaaaattac cctgatttgg tcaatatgtg
     8761 tacacatatt aaaacattac actttaaccc ataaatatgt ataatgatta tgtatcaatt
     8821 aaaaataaaa gaaaataaag tagggagatt atgaatatgc aaataagcac acatatattc
     8881 caaatagtaa tgtactaggc agactgtgta aagttttttt ttaagttact taatgtatct
     8941 cagagatatt tccttttgtt atacacaatg ttaaggcatt aagtataata gtaaaaattg
     9001 cggagaagaa aaaaaaagaa agcaagaatt aaacaaaaga aaacaattgt tatgaacagc
     9061 aaataaaaga aactaaaacg atcctgagac ttccacactg atgcaatcat tcgtctgttt
     9121 cccattctaa actgtaccct gttacttctc cccttcctat gacatgaact taaccataga
     9181 aaagaagggg aaagaaaaca tcaagggtcc catagactca ccctgaagtt ctcaggatcc
     9241 acgtgcagct tgtcacagtg cagctcactc agtgtggcaa aggtgccctt gaggttgtcc
     9301 aggtgagcca ggccatcact aaaggcaccg agcactttct tgccatgagc cttcacctta
     9361 gggttgccca taacagcatc aggagtggac agatccccaa aggactcaaa gaacctctgg
     9421 gtccaagggt agaccaccag cagcctaagg gtgggaaaat agaccaatag gcagagagag
     9481 tcagtgccta tcagaaaccc aagagtcttc tctgtctcca catgcccagt ttctattggt
     9541 ctccttaaac ctgtcttgta accttgatac caacctgccc agggcctcac caccaacttc
     9601 atccacgttc accttgcccc acagggcagt aacggcagac ttctcctcag gagtcaggtg
     9661 caccatggtg tctgtttgag gttgctagtg aacacagttg tgtcagaagc aaatgtaagc
     9721 aatagatggc tctgccctga cttttatgcc cagccctggc tcctgccctc cctgctcctg
     9781 ggagtagatt ggccaaccct agggtgtggc tccacagggt gaggtctaag tgatgacagc
     9841 cgtacctgtc cttggctctt ctggcactgg cttaggagtt ggacttcaaa ccctcagccc
     9901 tccctctaag atatatctct tggccccata ccatcagtac aaattgctac taaaaacatc
     9961 ctcctttgca agtgtattta cgcttctttg agaaacatct tcttcgttag tggcctgccc
    10021 ctcattccca ctttaatatc cagaatcact ataagaagaa tataataaga ggaataactc
    10081 ttattatagg taagggaaaa ttaagaggca tacgtgatgg gatgagtaag agaggagagg
    10141 gaaggattaa tggatgataa aatctactac tatttgttga gaccttttat agtctaatca
    10201 attttgctat tgttttccat cctcacgcta actccataaa aaaacactat tattatcttt
    10261 attttgccat gacaagactg agctcagaag agtcaagcat ttgcctaagg tcggacatgt
    10321 cagaggcagt gccagaccta tgtgagactc tgcagctact gctcatgggc cctgtgctgc
    10381 actgatgagg aggatcagat ggatggggca atgaagcaaa ggaatcattc tgtggataaa
    10441 ggagacagcc atgaagaagt ctatgactgt aaatttggga gcaggagtct ctaaggactt
    10501 ggatttcaag gaattttgac tcagcaaaca caagaccctc acggtgactt tgcgagctgg
    10561 tgtgccagat gtgtctatca gaggttccag ggagggtggg gtggggtcag ggctggccac
    10621 cagctatcag ggcccagatg ggttataggc tggcaggctc agataggtgg ttaggtcagg
    10681 ttggtggtgc tgggtggagt ccatgactcc caggagccag gagagataga ccatgagtag
    10741 agggcagaca tgggaaaggt gggggaggca cagcatagca gcatttttca ttctactact
    10801 acatgggact gctcccctat acccccagct aggggcaagt gccttgactc ctatgttttc
    10861 aggatcatca tctataaagt aagagtaata attgtgtcta tctcataggg ttattatgag
    10921 gatcaaagga gatgcacact ctctggacca gtggcctaac agttcaggac agagctatgg
    10981 gcttcctatg tatgggtcag tggtctcaat gtagcaggca agttccagaa gatagcatca
    11041 accactgtta gagatatact gccagtctca gagcctgatg ttaatttagc aatgggctgg
    11101 gaccctcctc cagtagaacc ttctaaccag ctgctgcagt caaagtcgaa tgcagctggt
    11161 tagacttttt ttaatgaaag cttgtaccag tggggcctct aagactaagt cactctgtct
    11221 caccgtgtct tagccagttc cttacagctt gccctgatgg gagatagaga atgggtatcc
    11281 tccaacaaaa aaataaattt tcatttctca aggtccaact tatgttttct taatttttaa
    11341 aaaaatcttg accattctcc actctctaaa ataatccaca gtgagagaaa cattcttttc
    11401 ccccatccca taaatacctc tattaaatat ggaaaatctg ggcatggtgt ctcacacctg
    11461 taatcccagc actttgggag gctgaggtgg gtggactgct tggagctcag gagttcaaga
    11521 ccatcttgga caacatggtg ataccctgcc tctacaaaaa gtacaaaaat tagcctggca
    11581 tggtggtgtg cacctgtaat cccagctatt agggtggctg aggcaggaga attgcttgaa
    11641 cccgggaggc ggaggttgca gtgagctgag atcgtgccac tgcactccag cctgggggac
    11701 agagcacatt ataattaact gttatttttt acttggactc ttgtggggaa taagatacat
    11761 gttttattct tatttatgat tcaagcactg aaaatagtgt ttagcatcca gcaggtgctt
    11821 caaaaccatt tgctgaatga ttactatact ttttacaagc tcagctccct ctatcccttc
    11881 cagcatcctc atctctgatt aaataagctt cagtttttcc ttagttcctg ttacatttct
    11941 gtgtgtctcc attagtgacc tcccatagtc caagcatgag cagttctggc caggcccctg
    12001 tcggggtcag tgccccaccc ccgccttctg gttctgtgta accttctaag caaaccttct
    12061 ggctcaagca cagcaatgct gagtcatgat gagtcatgct gaggcttagg gtgtgtgccc
    12121 agatgttctc agcctagagt gatgactcct atctgggtcc ccagcaggat gcttacaggg
    12181 cagatggcaa aaaaaaggag aagctgacca cctgactaaa actccacctc aaacggcatc
    12241 ataaagaaaa tggatgcctg agacagaatg tgacatattc tagaatatat tatttcctga
    12301 atatatatat atatatatat acatatacgt atatatatat atatatatat atttgttgtt
    12361 atcaattgcc atagaatgat tagttattgt gaatcaaata tttatcttgc aggtggcctc
    12421 tatacctaga agcggcagaa tcaggcttta ttaatacatg tgtatagatt tttaggatct
    12481 atacacatgt attaatatga aacaaggata tggaagagga aggcatgaaa acaggaaaag
    12541 aaaacaaacc ttgtttgcca ttttaaggca cccctggaca gctaggtggc aaaaggaagc
    12601 ttccctttgg tctccccctc ttaagtacca catttgtaga ggttttactt gctttaaaaa
    12661 acctcccaca cctccccctg aacct
//
