LOCUS       sequence-445702-       12648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tc1-2_ST Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 12648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-Tc1-2_ST-10 kb
FEATURES             Location/Qualifiers
     source          1..12648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    384..465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(421..440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2008..2031)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2031..2040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2055..3233)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2064..3002
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(2087..2107)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    3279..3503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3492..3509
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(3531..4244,4245..4247,4248..4250))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3534..4244,4245..4247,4248..4250))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3576..3597)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3925..3944
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4184..4205
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(4251..4307)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4314..4913)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4394..4414)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     regulatory      4910..4919
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4933..5432)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4981..5000)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5374..5397
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(5768..5785)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5768..5784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5794..5812)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5880..5984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5985..6053,6054..6845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6203..6222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7016..7604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7505..7524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7758..7775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7787..7803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7787..7803
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8163..8557
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 tatatatata tatatatatt tgttgttatc aattgccata gaatgattag ttattgtgaa
       61 tcaaatattt atcttgcagg tggcctctat acctagaagc ggcagaatca ggctttatta
      121 atacatgtgt atagattttt aggatctata cacatgtatt aatatgaaac aaggatatgg
      181 aagaggaagg catgaaaaca ggaaaagaaa acaaaccttg tttgccattt taaggcaccc
      241 ctggacagct aggtggcaaa aggaagcttc cctttggtct ccccctctta agtaccacat
      301 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      361 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      421 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      481 tgtccaaact caatcgatgc gagggggcag ggcctgcatg tgaagggcgt cgtaggtgtc
      541 cttggtggct gtactgagac cctggtaaag gccatcgtgc cccttgcccc tccggcgctc
      601 gcctttcatc ccaatctcac tgtaggcctc cgccatctta tctttctgca gttcattgta
      661 caggccttcc tgagggttct tccttctcgg ctttcccccc atctcagggt cccggccacg
      721 tctcttgtcc aaaacatcgt actcctctct tcgtcctaga ttgagctcgt tatagagctg
      781 gttctggccc tgctggtacg cgggggcgtc tgcgctcctg ctgaacttca ctctggagcg
      841 ataggctgcg aagtcgcgtg gtggggcata gggctggtaa tgcttgcggg tgggcccggg
      901 gcggcgggga gtcatgttca tgtagtcact gtgcaggagc ctgctcctct tactcctcac
      961 ccagaaaata ataaaggcca ctgttactag caagctatag caagccagga ctcccccaac
     1021 caccaccagc acccaaaaat cacaggcgaa gtccagcccc ctcgtgtgca ctgcgccccc
     1081 cgccgctggc cggcacgcct ctgggcgcag ggacaggggc tgcgacgcga tggtgggcgc
     1141 cggtgttggt ggtcgcggcg ctggcgtcgt ggtcagcact gtcagttgcg tgcctccacc
     1201 gaaaacagcg gctgaactat gccaaatcat acagtaataa tcggcctcgt cctcggacct
     1261 gagacctgag atgagcagca cacccgcatt ggcgcttgcg tctttagatc cactaaacct
     1321 tgatggaact ccagatccct gctgcttatc tgagtcgctc ttgtaattga gaaggtattg
     1381 gggaggagaa cctggttttt gctgatacca gtagatcctg taaggcccta cattgattcc
     1441 tgacctcagt gtacacgtca aagaagcgga ggcaccgggg cttgcactga gggaggatga
     1501 ttgcgtgaga acaggttggc tacccccccc tccggacccg cccccaccgg aacctccacc
     1561 gcctgagccc aggatcccac tggacacggt gacggttgta ccttgtcccc aaacgtccat
     1621 accatagtag taagtcatca taccccgcgc acaatagtaa acagctgtgt cttcgggagt
     1681 tacactatta agctgaagag agaactggtt tttggaagta tctggattta tagacatcct
     1741 gcttttcaca cttacagcat aatcattata ccatttactg cggtagtatg ttctaccgag
     1801 ccattccaac cctcgactgg gggactgcct gatccaattc catgtcgcgg agttactact
     1861 cactgaatca cctgatatgg cacaggtaag actaagagtc tgggaaggag tgaccagacc
     1921 ggggccagat tgttggagtt gcacttgcgg cctggcggcg tggagcagca aggccagcgg
     1981 caggagcaag gcggtcactg gtaaggcctt atcgtcatcg tctttgtaat ccatggtggc
     2041 gaccggtgga tccctcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
     2101 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
     2161 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
     2221 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
     2281 tgcctggacg gcgcccggta ctcagtggag tcacatgaag cgacggctga ggacggaaag
     2341 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
     2401 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
     2461 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
     2521 agagcaggcc ggcgagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
     2581 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
     2641 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
     2701 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
     2761 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
     2821 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
     2881 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
     2941 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
     3001 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     3061 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     3121 cagtttaccc cgcgccacct tctctaggca ccgggtcaat tgccgacccc tccccccaac
     3181 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcctcacgtt
     3241 aattaaccaa ggtacccatt ggcggccgcc ctcagaagcc atagagccca ccgcatcccc
     3301 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     3361 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     3421 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     3481 tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gcttaattaa ttacttgtac
     3541 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     3601 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     3661 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     3721 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     3781 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     3841 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     3901 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     3961 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     4021 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     4081 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     4141 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     4201 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat tggcccggga
     4261 ttctcttcga catcccctgc ttgtttcaac agggagaagt tagtggcatc gatggcaccg
     4321 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
     4381 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     4441 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     4501 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
     4561 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
     4621 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     4681 gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     4741 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg
     4801 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg
     4861 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtccc catggtggcg
     4921 ttggctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     4981 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     5041 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     5101 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     5161 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     5221 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     5281 gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     5341 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
     5401 agactgcctt gggaaaagcg cctcccctac ccggtagaat tgctagcacc ggttttgaag
     5461 gctcaggaaa cctttgcagg tgttttgagt tgattagctg attggcatgt caccatattc
     5521 taatttgttg agatagtgaa ttggtgggtt tttgttaaat gtgagccaaa atcatcacaa
     5581 ttaaaagaac caaagactta aactacttca gtctgtgtgc attgaattta tttaatacac
     5641 gagtttcaca atttgagttg aattactgaa ataaatgaac ttttccacga cattctaatt
     5701 tattgagatg cacctgtaca attgagagac gcaagacact gcggatcgag acgcattgcg
     5761 tggttcgact ggccgtcgtt ttacaacgaa agggcctcgt gatacgccta tttttatagg
     5821 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5881 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5941 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6001 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6061 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6121 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6181 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6241 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6301 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgtagt gctgccataa
     6361 ccatgagtga taacacggcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6421 taaccgcttt tttgcgcaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6481 agctgaatga agccatacca aacgacgagc gtgataccac gatgcctgta gcgatggcaa
     6541 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6601 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6661 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtcccgcggt attattgcag
     6721 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6781 caactatgga tgaacgaaag agacagatcg ctgagatagg tgcctcactg attaagcatt
     6841 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6901 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6961 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7021 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7081 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7141 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7201 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7261 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7321 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7381 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7441 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7501 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7561 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7621 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7681 cccctgattc tgtgggtaac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7741 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggcacacagg aaacagctat
     7801 gaccgtctcg gttggcagtg actccgtctc tgggccctac aggtgcatct caataaatta
     7861 gaatgtcgtg gaaaagttca tttatttcag taattcaact caaattgtga aactcgtgta
     7921 ttaaataaat tcaatgcaca cagactgaag tagtttaagt ctttggttct tttaattgtg
     7981 atgattttgg ctcacattta acaaaaaccc accaattcac tatctcaaca aattagaata
     8041 cttcataaga ccaataaaaa aaacattttt agtgaattgt tggccttctg gaaagtatgt
     8101 tcatttactg tacatgtacc tcgagggcgc gcccatgagg acagctaaaa caataagtaa
     8161 tgtaaaatac agcatagcaa aactttaacc tccaaatcaa gcctctactt gaatcctttt
     8221 ctgagggatg aataaggcat aggcatcagg ggctgttgcc aatgtgcatt agctgtttgc
     8281 agcctcacct tctttcatgg agtttaagat atagtgtatt ttcccaaggt ttgaactagc
     8341 tcttcatttc tttatgtttt aaatgcactg acctcccaca ttcccttttt agtaaaatat
     8401 tcagaaataa tttaaataca tcattgcaat gaaaataaat gttttttatt aggcagaatc
     8461 cagatgctca aggcccttca taatatcccc cagtttagta gttggactta gggaacaaag
     8521 gaacctttaa tagaaattgg acagcaagaa agcgagctta gtgatacttg tgggccaggg
     8581 cattagccac accagccacc actttctgat aggcagcctg cactggtggg gtgaattctt
     8641 tgccaaagtg atgggccagc acacagacca gcacgttgcc caggagctgt gggaggaaga
     8701 taagaggtat gaacatgatt agcaaaaggg cctagcttgg actcagaata atccagcctt
     8761 atcccaacca taaaataaaa gcagaatggt agctggattg tagctgctat tagcaatatg
     8821 aaacctctta catcagttac aatttatatg cagaaatatt tatatgcaga aatattgcta
     8881 ttgccttaac ccagaaatta tcactgttat tctttagaat ggtgcaaaga ggcatgatac
     8941 attgtatcat tattgccctg aaagaaagag attagggaaa gtattagaaa taagataaac
     9001 aaaaaagtat attaaaagaa gaaagcattt tttaaaatta caaatgcaaa attaccctga
     9061 tttggtcaat atgtgtacac atattaaaac attacacttt aacccataaa tatgtataat
     9121 gattatgtat caattaaaaa taaaagaaaa taaagtaggg agattatgaa tatgcaaata
     9181 agcacacata tattccaaat agtaatgtac taggcagact gtgtaaagtt tttttttaag
     9241 ttacttaatg tatctcagag atatttcctt ttgttataca caatgttaag gcattaagta
     9301 taatagtaaa aattgcggag aagaaaaaaa aagaaagcaa gaattaaaca aaagaaaaca
     9361 attgttatga acagcaaata aaagaaacta aaacgatcct gagacttcca cactgatgca
     9421 atcattcgtc tgtttcccat tctaaactgt accctgttac ttctcccctt cctatgacat
     9481 gaacttaacc atagaaaaga aggggaaaga aaacatcaag ggtcccatag actcaccctg
     9541 aagttctcag gatccacgtg cagcttgtca cagtgcagct cactcagtgt ggcaaaggtg
     9601 cccttgaggt tgtccaggtg agccaggcca tcactaaagg caccgagcac tttcttgcca
     9661 tgagccttca ccttagggtt gcccataaca gcatcaggag tggacagatc cccaaaggac
     9721 tcaaagaacc tctgggtcca agggtagacc accagcagcc taagggtggg aaaatagacc
     9781 aataggcaga gagagtcagt gcctatcaga aacccaagag tcttctctgt ctccacatgc
     9841 ccagtttcta ttggtctcct taaacctgtc ttgtaacctt gataccaacc tgcccagggc
     9901 ctcaccacca acttcatcca cgttcacctt gccccacagg gcagtaacgg cagacttctc
     9961 ctcaggagtc aggtgcacca tggtgtctgt ttgaggttgc tagtgaacac agttgtgtca
    10021 gaagcaaatg taagcaatag atggctctgc cctgactttt atgcccagcc ctggctcctg
    10081 ccctccctgc tcctgggagt agattggcca accctagggt gtggctccac agggtgaggt
    10141 ctaagtgatg acagccgtac ctgtccttgg ctcttctggc actggcttag gagttggact
    10201 tcaaaccctc agccctccct ctaagatata tctcttggcc ccataccatc agtacaaatt
    10261 gctactaaaa acatcctcct ttgcaagtgt atttacgctt ctttgagaaa catcttcttc
    10321 gttagtggcc tgcccctcat tcccacttta atatccagaa tcactataag aagaatataa
    10381 taagaggaat aactcttatt ataggtaagg gaaaattaag aggcatacgt gatgggatga
    10441 gtaagagagg agagggaagg attaatggat gataaaatct actactattt gttgagacct
    10501 tttatagtct aatcaatttt gctattgttt tccatcctca cgctaactcc ataaaaaaac
    10561 actattatta tctttatttt gccatgacaa gactgagctc agaagagtca agcatttgcc
    10621 taaggtcgga catgtcagag gcagtgccag acctatgtga gactctgcag ctactgctca
    10681 tgggccctgt gctgcactga tgaggaggat cagatggatg gggcaatgaa gcaaaggaat
    10741 cattctgtgg ataaaggaga cagccatgaa gaagtctatg actgtaaatt tgggagcagg
    10801 agtctctaag gacttggatt tcaaggaatt ttgactcagc aaacacaaga ccctcacggt
    10861 gactttgcga gctggtgtgc cagatgtgtc tatcagaggt tccagggagg gtggggtggg
    10921 gtcagggctg gccaccagct atcagggccc agatgggtta taggctggca ggctcagata
    10981 ggtggttagg tcaggttggt ggtgctgggt ggagtccatg actcccagga gccaggagag
    11041 atagaccatg agtagagggc agacatggga aaggtggggg aggcacagca tagcagcatt
    11101 tttcattcta ctactacatg ggactgctcc cctatacccc cagctagggg caagtgcctt
    11161 gactcctatg ttttcaggat catcatctat aaagtaagag taataattgt gtctatctca
    11221 tagggttatt atgaggatca aaggagatgc acactctctg gaccagtggc ctaacagttc
    11281 aggacagagc tatgggcttc ctatgtatgg gtcagtggtc tcaatgtagc aggcaagttc
    11341 cagaagatag catcaaccac tgttagagat atactgccag tctcagagcc tgatgttaat
    11401 ttagcaatgg gctgggaccc tcctccagta gaaccttcta accagctgct gcagtcaaag
    11461 tcgaatgcag ctggttagac tttttttaat gaaagcttgt accagtgggg cctctaagac
    11521 taagtcactc tgtctcaccg tgtcttagcc agttccttac agcttgccct gatgggagat
    11581 agagaatggg tatcctccaa caaaaaaata aattttcatt tctcaaggtc caacttatgt
    11641 tttcttaatt tttaaaaaaa tcttgaccat tctccactct ctaaaataat ccacagtgag
    11701 agaaacattc ttttccccca tcccataaat acctctatta aatatggaaa atctgggcat
    11761 ggtgtctcac acctgtaatc ccagcacttt gggaggctga ggtgggtgga ctgcttggag
    11821 ctcaggagtt caagaccatc ttggacaaca tggtgatacc ctgcctctac aaaaagtaca
    11881 aaaattagcc tggcatggtg gtgtgcacct gtaatcccag ctattagggt ggctgaggca
    11941 ggagaattgc ttgaacccgg gaggcggagg ttgcagtgag ctgagatcgt gccactgcac
    12001 tccagcctgg gggacagagc acattataat taactgttat tttttacttg gactcttgtg
    12061 gggaataaga tacatgtttt attcttattt atgattcaag cactgaaaat agtgtttagc
    12121 atccagcagg tgcttcaaaa ccatttgctg aatgattact atacttttta caagctcagc
    12181 tccctctatc ccttccagca tcctcatctc tgattaaata agcttcagtt tttccttagt
    12241 tcctgttaca tttctgtgtg tctccattag tgacctccca tagtccaagc atgagcagtt
    12301 ctggccaggc ccctgtcggg gtcagtgccc cacccccgcc ttctggttct gtgtaacctt
    12361 ctaagcaaac cttctggctc aagcacagca atgctgagtc atgatgagtc atgctgaggc
    12421 ttagggtgtg tgcccagatg ttctcagcct agagtgatga ctcctatctg ggtccccagc
    12481 aggatgctta cagggcagat ggcaaaaaaa aggagaagct gaccacctga ctaaaactcc
    12541 acctcaaacg gcatcataaa gaaaatggat gcctgagaca gaatgtgaca tattctagaa
    12601 tatattattt cctgaatata tatatatata tatatacata tacgtata
//
