LOCUS       sequence-445703-       13512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of piggyBac-1_AMi Transposon with 10 kb cargo gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 13512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-piggyBac-1_AMi-10 kb
FEATURES             Location/Qualifiers
     source          1..13512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2841..3235
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3687..3703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3687..3703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3715..3732)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3886..4474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3966..3985)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4645..5436,5437..5505))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     5268..5287
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5506..5610)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5678..5696
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     5705..5722
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5706..5722
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6804..6813
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6830..7329
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6865..6888)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7262..7281
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      7343..7352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7349..7948
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7848..7868
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7955..8011
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8012..8014,8015..8017,8018..8731)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8012..8014,8015..8017,8018..8728)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8057..8078)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8318..8337)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8665..8686
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8753..8770)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8759..8983
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9029..10207
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9260..10198
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10155..10175
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      10222..10231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10231..10254
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    11797..11878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11822..11841
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aacatcaggc tctgagactg gcagtatatc tctaacagtg gttgatgcta tcttctggaa
       61 cttgcctgct acattgagac cactgaccca tacataggaa gcccatagct ctgtcctgaa
      121 ctgttaggcc actggtccag agagtgtgca tctcctttga tcctcataat aaccctatga
      181 gatagacaca attattactc ttactttata gatgatgatc ctgaaaacat aggagtcaag
      241 gcacttgccc ctagctgggg gtatagggga gcagtcccat gtagtagtag aatgaaaaat
      301 gctgctatgc tgtgcctccc ccacctttcc catgtctgcc ctctactcat ggtctatctc
      361 tcctggctcc tgggagtcat ggactccacc cagcaccacc aacctgacct aaccacctat
      421 ctgagcctgc cagcctataa cccatctggg ccctgatagc tggtggccag ccctgacccc
      481 accccaccct ccctggaacc tctgatagac acatctggca caccagctcg caaagtcacc
      541 gtgagggtct tgtgtttgct gagtcaaaat tccttgaaat ccaagtcctt agagactcct
      601 gctcccaaat ttacagtcat agacttcttc atggctgtct cctttatcca cagaatgatt
      661 cctttgcttc attgccccat ccatctgatc ctcctcatca gtgcagcaca gggcccatga
      721 gcagtagctg cagagtctca cataggtctg gcactgcctc tgacatgtcc gaccttaggc
      781 aaatgcttga ctcttctgag ctcagtcttg tcatggcaaa ataaagataa taatagtgtt
      841 tttttatgga gttagcgtga ggatggaaaa caatagcaaa attgattaga ctataaaagg
      901 tctcaacaaa tagtagtaga ttttatcatc cattaatcct tccctctcct ctcttactca
      961 tcccatcacg tatgcctctt aattttccct tacctataat aagagttatt cctcttatta
     1021 tattcttctt atagtgattc tggatattaa agtgggaatg aggggcaggc cactaacgaa
     1081 gaagatgttt ctcaaagaag cgtaaataca cttgcaaaga aggatgtttt tagtagcaat
     1141 ttgtactgat ggtatggggc caagagatat atcttagagg gagggctgag ggtttgaagt
     1201 ccaactccta agccagtgcc agaagagcca aggacaggta cggctgtcat cacttagacc
     1261 tcaccctgtg gagccacacc ctagggttgg ccaatctact cccaggagca gggagggcag
     1321 gagccagggc tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctga
     1381 cacaactgtg ttcactagca acctcaaaca gacaccatgg tgcacctgac tcctgaggag
     1441 aagtctgccg ttactgccct gtggggcaag gtgaacgtgg atgaagttgg tggtgaggcc
     1501 ctgggcaggt tggtatcaag gttacaagac aggtttaagg agaccaatag aaactgggca
     1561 tgtggagaca gagaagactc ttgggtttct gataggcact gactctctct gcctattggt
     1621 ctattttccc acccttaggc tgctggtggt ctacccttgg acccagaggt tctttgagtc
     1681 ctttggggat ctgtccactc ctgatgctgt tatgggcaac cctaaggtga aggctcatgg
     1741 caagaaagtg ctcggtgcct ttagtgatgg cctggctcac ctggacaacc tcaagggcac
     1801 ctttgccaca ctgagtgagc tgcactgtga caagctgcac gtggatcctg agaacttcag
     1861 ggtgagtcta tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg
     1921 tcataggaag gggagaagta acagggtaca gtttagaatg ggaaacagac gaatgattgc
     1981 atcagtgtgg aagtctcagg atcgttttag tttcttttat ttgctgttca taacaattgt
     2041 tttcttttgt ttaattcttg ctttcttttt ttttcttctc cgcaattttt actattatac
     2101 ttaatgcctt aacattgtgt ataacaaaag gaaatatctc tgagatacat taagtaactt
     2161 aaaaaaaaac tttacacagt ctgcctagta cattactatt tggaatatat gtgtgcttat
     2221 ttgcatattc ataatctccc tactttattt tcttttattt ttaattgata cataatcatt
     2281 atacatattt atgggttaaa gtgtaatgtt ttaatatgtg tacacatatt gaccaaatca
     2341 gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt
     2401 tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta
     2461 tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag
     2521 caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat
     2581 attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag
     2641 gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct
     2701 tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag
     2761 aattcacccc accagtgcag gctgcctatc agaaagtggt ggctggtgtg gctaatgccc
     2821 tggcccacaa gtatcactaa gctcgctttc ttgctgtcca atttctatta aaggttcctt
     2881 tgttccctaa gtccaactac taaactgggg gatattatga agggccttga gcatctggat
     2941 tctgcctaat aaaaaacatt tattttcatt gcaatgatgt atttaaatta tttctgaata
     3001 ttttactaaa aagggaatgt gggaggtcag tgcatttaaa acataaagaa atgaagagct
     3061 agttcaaacc ttgggaaaat acactatatc ttaaactcca tgaaagaagg tgaggctgca
     3121 aacagctaat gcacattggc aacagcccct gatgcctatg ccttattcat ccctcagaaa
     3181 aggattcaag tagaggcttg atttggaggt taaagttttg ctatgctgta ttttacttct
     3241 gaagcgcttg tacacatatg gtcgacctgc agaattcact agtggcgcgc cctcgagtta
     3301 aaacagttag gaaatgtttt tggaagtaat tgttagtttg aaaacagtgt gaactgcaga
     3361 cttagcaaat tgttcattca cgttttgaaa ctctaaaaaa agtttaaatt ttgttcatag
     3421 ttctagttaa ataatatttt gggttcaaat catcataaat actctttcaa acaatctgta
     3481 tgtttaataa atagtttttg gtacatttat actgttgtca ctggttccat tttgttcatg
     3541 cgggtggggt aacaagttac cccatgagac caacaatgtc gaattgcggg gtaacaagtt
     3601 accccatgag aacaacaacg gttacggttt catgagaacg gatgagggtt aagggcccag
     3661 agacggagtc actgccaacc gagacggtca tagctgtttc ctgtgtgccg cttcctcgct
     3721 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3781 ggtaatacgg ttacccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3841 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3901 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3961 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4021 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4081 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4141 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4201 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4261 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4321 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4381 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4441 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4501 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4561 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4621 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4681 gatctgtctc tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4741 acgggagggc ttaccatctg gccccagtgc tgcaataata ccgcgggacc cacgctcacc
     4801 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4861 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4921 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtatcacg
     4981 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5041 atcccccatg ttgcgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5101 taagttggcc gccgtgttat cactcatggt tatggcagca ctacataatt ctcttactgt
     5161 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5221 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5281 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5341 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5401 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5461 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5521 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5581 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5641 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     5701 tcgttgtaaa acgacggcca gtcgaaccac gcaatgcgtc tcgatccgca gtgtcttgcg
     5761 tctctcaatt gttaaccttc atacgttccc atggggtaat ttcctacccc agacgcatat
     5821 tttgtgcagt atcttaaagt taattaattc taaaaaatat tctttttagg acattgtcac
     5881 tggcaccgca gtgcacaata tccccaaaga acagccagct tacatgaatg gtttgctctc
     5941 tgtggatgaa aaactgccca gggtaacatg ttaccccagg cgtgaatgtg tttttccata
     6001 tggggtaaca acttacccca agcccatgtt aacgcattgg ggtaacttgt taccccaatg
     6061 caatttctgt ataaatgaca ggcctagacc tgtttggcca ctttgggtgg atgcatctga
     6121 aaggtcagca ttcattcaca cctcattcat gtccacttca ggtatccatg gtaactaatg
     6181 gatggccatc ctccactctg cttttgcaga agatgtggta atcccttttg ctaaccactt
     6241 tgtttgcagg ctgcaaggtc atcaaaacac cctgcccttt gccaaaaaaa cccaaaaccc
     6301 tttgtcacac ccaaatccac ctctgctctc ttttctcatg gcaaaactcc gcctcacctg
     6361 cccctttcat aacatacaag ctagtttgcc ctgcctttgt tggaagtgag gacaaggagc
     6421 aaagggacag cagagaagaa atagaacaac atcatactgc aatacagcgt acactatgga
     6481 gtccccttca acggtaaggg cttgacaccc cccatgcccc ttttacctgc tctcaataag
     6541 cccctgcatg tgtggtttcc aaacagcatc cacccacatt tggcttgcct gcaaagggtg
     6601 gatgctggtc tctggcttct gtgcccagga ggagagggtg ctacctgcgc tgccataggg
     6661 cagggcggaa ttgcttctgt gacagcatag gtggacaggc ttgtctttga ttgttattta
     6721 tatgcattca gcagtaatca tttttttttt ttttttctgc agtccaggac cggtgctagc
     6781 gggcccgacg tcgcatgctc ccggccgcca tgggtataca attctaccgg gtagggggag
     6841 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     6901 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     6961 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     7021 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     7081 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     7141 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     7201 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     7261 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     7321 ggcctttcga cctgcagcca acgccaccat ggggaccgag tacaagccca cggtgcgcct
     7381 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     7441 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     7501 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7561 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7621 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7681 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7741 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7801 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7861 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7921 ctggtgcatg acccgcaagc ccggtgccat cgatgccact aacttctccc tgttgaaaca
     7981 agcaggggat gtcgaagaga atcccgggcc aatggtgagc aagggcgagg agctgttcac
     8041 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     8101 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     8161 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     8221 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     8281 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     8341 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     8401 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     8461 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     8521 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     8581 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     8641 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     8701 cactctcggc atggacgagc tgtacaagta attaattaag ctcgctgatc agcctcgact
     8761 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8821 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8881 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8941 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga gggcggccgc
     9001 caatgggtac cttggttaat taacgtgagg ctccggtgcc cgtcagtggg cagagcgcac
     9061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgacccg gtgcctagag
     9121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     9181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     9241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     9301 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     9361 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     9421 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     9481 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     9541 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     9601 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     9661 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     9721 tcaagctcgc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     9781 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     9841 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     9901 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccactga
     9961 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
    10021 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
    10081 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
    10141 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
    10201 gtcgtgaggg atccaccggt cgccaccatg gattacaaag acgatgacga taaggcctta
    10261 ccagtgaccg ccttgctcct gccgctggcc ttgctgctcc acgccgccag gccgcaagtg
    10321 caactccaac aatctggccc cggtctggtc actccttccc agactcttag tcttacctgt
    10381 gccatatcag gtgattcagt gagtagtaac tccgcgacat ggaattggat caggcagtcc
    10441 cccagtcgag ggttggaatg gctcggtaga acatactacc gcagtaaatg gtataatgat
    10501 tatgctgtaa gtgtgaaaag caggatgtct ataaatccag atacttccaa aaaccagttc
    10561 tctcttcagc ttaatagtgt aactcccgaa gacacagctg tttactattg tgcgcggggt
    10621 atgatgactt actactatgg tatggacgtt tggggacaag gtacaaccgt caccgtgtcc
    10681 agtgggatcc tgggctcagg cggtggaggt tccggtgggg gcgggtccgg aggggggggt
    10741 agccaacctg ttctcacgca atcatcctcc ctcagtgcaa gccccggtgc ctccgcttct
    10801 ttgacgtgta cactgaggtc aggaatcaat gtagggcctt acaggatcta ctggtatcag
    10861 caaaaaccag gttctcctcc ccaatacctt ctcaattaca agagcgactc agataagcag
    10921 cagggatctg gagttccatc aaggtttagt ggatctaaag acgcaagcgc caatgcgggt
    10981 gtgctgctca tctcaggtct caggtccgag gacgaggccg attattactg tatgatttgg
    11041 catagttcag ccgctgtttt cggtggaggc acgcaactga cagtgctgac cacgacgcca
    11101 gcgccgcgac caccaacacc ggcgcccacc atcgcgtcgc agcccctgtc cctgcgccca
    11161 gaggcgtgcc ggccagcggc ggggggcgca gtgcacacga gggggctgga cttcgcctgt
    11221 gatttttggg tgctggtggt ggttggggga gtcctggctt gctatagctt gctagtaaca
    11281 gtggccttta ttattttctg ggtgaggagt aagaggagca ggctcctgca cagtgactac
    11341 atgaacatga ctccccgccg ccccgggccc acccgcaagc attaccagcc ctatgcccca
    11401 ccacgcgact tcgcagccta tcgctccaga gtgaagttca gcaggagcgc agacgccccc
    11461 gcgtaccagc agggccagaa ccagctctat aacgagctca atctaggacg aagagaggag
    11521 tacgatgttt tggacaagag acgtggccgg gaccctgaga tggggggaaa gccgagaagg
    11581 aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag ataagatggc ggaggcctac
    11641 agtgagattg ggatgaaagg cgagcgccgg aggggcaagg ggcacgatgg cctttaccag
    11701 ggtctcagta cagccaccaa ggacacctac gacgcccttc acatgcaggc cctgccccct
    11761 cgcatcgatt gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
    11821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
    11881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
    11941 aagcaagtaa aacctctaca aatgtggtac ttaagagggg gagaccaaag ggaagcttcc
    12001 ttttgccacc tagctgtcca ggggtgcctt aaaatggcaa acaaggtttg ttttcttttc
    12061 ctgttttcat gccttcctct tccatatcct tgtttcatat taatacatgt gtatagatcc
    12121 taaaaatcta tacacatgta ttaataaagc ctgattctgc cgcttctagg tatagaggcc
    12181 acctgcaaga taaatatttg attcacaata actaatcatt ctatggcaat tgataacaac
    12241 aaatatatat atatatatat atatacgtat atgtatatat atatatatat atattcagga
    12301 aataatatat tctagaatat gtcacattct gtctcaggca tccattttct ttatgatgcc
    12361 gtttgaggtg gagttttagt caggtggtca gcttctcctt ttttttgcca tctgccctgt
    12421 aagcatcctg ctggggaccc agataggagt catcactcta ggctgagaac atctgggcac
    12481 acaccctaag cctcagcatg actcatcatg actcagcatt gctgtgcttg agccagaagg
    12541 tttgcttaga aggttacaca gaaccagaag gcgggggtgg ggcactgacc ccgacagggg
    12601 cctggccaga actgctcatg cttggactat gggaggtcac taatggagac acacagaaat
    12661 gtaacaggaa ctaaggaaaa actgaagctt atttaatcag agatgaggat gctggaaggg
    12721 atagagggag ctgagcttgt aaaaagtata gtaatcattc agcaaatggt tttgaagcac
    12781 ctgctggatg ctaaacacta ttttcagtgc ttgaatcata aataagaata aaacatgtat
    12841 cttattcccc acaagagtcc aagtaaaaaa taacagttaa ttataatgtg ctctgtcccc
    12901 caggctggag tgcagtggca cgatctcagc tcactgcaac ctccgcctcc cgggttcaag
    12961 caattctcct gcctcagcca ccctaatagc tgggattaca ggtgcacacc accatgccag
    13021 gctaattttt gtactttttg tagaggcagg gtatcaccat gttgtccaag atggtcttga
    13081 actcctgagc tccaagcagt ccacccacct cagcctccca aagtgctggg attacaggtg
    13141 tgagacacca tgcccagatt ttccatattt aatagaggta tttatgggat gggggaaaag
    13201 aatgtttctc tcactgtgga ttattttaga gagtggagaa tggtcaagat ttttttaaaa
    13261 attaagaaaa cataagttgg accttgagaa atgaaaattt atttttttgt tggaggatac
    13321 ccattctcta tctcccatca gggcaagctg taaggaactg gctaagacac ggtgagacag
    13381 agtgacttag tcttagaggc cccactggta caagctttca ttaaaaaaag tctaaccagc
    13441 tgcattcgac tttgactgca gcagctggtt agaaggttct actggaggag ggtcccagcc
    13501 cattgctaaa tt
//
