LOCUS       sequence-44571-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Rosonina E, Blencowe BJ
  TITLE     Analysis of the requirement for RNA polymerase II CTD heptapeptide 
            repeats in pre-mRNA splicing and 3'-end cleavage.
  JOURNAL   RNA. 2004 Apr;10(4):581-9.
  PUBMED    15037767
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-04"; volume: "10(4)"; pages: "581-9"
COMMENT     Sequence Label: FLAG-Pol-II 15
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      653..662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctaggacc atggactaca aggacgacga tgacaaggct agcatgcacg
      121 ggggtggccc cccctcgggg gacagcgcat gcccgctgcg caccatcaag agagtccagt
      181 tcggagtcct gagtccggat gaactgaagc gaatgtctgt gacggagggt ggcatcaaat
      241 acccagagac gactgaggga ggccgcccca agcttggggg gctgatggac ccgaggcagg
      301 gggtgattga gcggactggc cgctgccaaa catgtgcagg aaacatgaca gagtgtcctg
      361 gccactttgg ccacattgaa ctggccaagc ctgtgtttca cgtgggcttc ctggtgaaga
      421 caatgaaagt tttgcgctgt gtctgcttct tctgctccaa actgcttgtg gactctaaca
      481 acccaaagat caaggatatc ctggctaagt ccaagggaca gcccaagaag cggctcacac
      541 atgtctacga cctttgcaag ggcaaaaaca tatgcgaggg tggggaggag atggacaaca
      601 agttcggtgt ggaacaacct gagggtgacg aggatctgac caaagaaaag ggccatggtg
      661 gctgtgggcg gtaccagccc aggatccggc gttctggcct agagctgtat gcggaatgga
      721 agcacgttaa tgaggactct caggagaaga agatcctgct gagtccagag cgagtgcatg
      781 agatcttcaa acgcatctca gatgaggagt gttttgtgct gggcatggag c
//
