LOCUS       sequence-44573-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Rosonina E, Blencowe BJ
  TITLE     Analysis of the requirement for RNA polymerase II CTD heptapeptide 
            repeats in pre-mRNA splicing and 3'-end cleavage.
  JOURNAL   RNA. 2004 Apr;10(4):581-9.
  PUBMED    15037767
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-04"; volume: "10(4)"; pages: "581-9"
COMMENT     Sequence Label: FLAG-Pol-II 1-22
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..106
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      652..661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctactgag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctg gctaggacca tggactacaa ggacgacgat gacaaggcta gcatgcacgg
      121 gggtggcccc ccctcggggg acagcgcatg cccgctgcgc accatcaaga gagtccagtt
      181 cggagtcctg agtccggatg aactgaagcg aatgtctgtg acggagggtg gcatcaaata
      241 cccagagacg actgagggag gccgccccaa gcttgggggg ctgatggacc cgaggcaggg
      301 ggtgattgag cggactggcc gctgccaaac atgtgcagga aacatgacag agtgtcctgg
      361 ccactttggc cacattgaac tggccaagcc tgtgtttcac gtgggcttcc tggtgaagac
      421 aatgaaagtt ttgcgctgtg tctgcttctt ctgctccaaa ctgcttgtgg actctaacaa
      481 cccaaagatc aaggatatcc tggctaagtc caagggacag cccaagaagc ggctcacaca
      541 tgtctacgac ctttgcaagg gcaaaaacat atgcgagggt ggggaggaga tggacaacaa
      601 gttcggtgtg gaacaacctg agggtgacga ggatctgacc aaagaaaagg gccatggtgg
      661 ctgtgggcgg taccagccca ggatccggcg ttctggccta gagctgtatg cggaatggaa
      721 gcacgttaat gaggactctc aggagaagaa gatcctgctg agtccagagc gagtgcatga
      781 gatcttcaaa cgcatctcag atgaggagtg ttttgtgctg ggcatgga
//
