LOCUS       sequence-44575-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Rosonina E, Blencowe BJ
  TITLE     Analysis of the requirement for RNA polymerase II CTD heptapeptide 
            repeats in pre-mRNA splicing and 3'-end cleavage.
  JOURNAL   RNA. 2004 Apr;10(4):581-9.
  PUBMED    15037767
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-04"; volume: "10(4)"; pages: "581-9"
COMMENT     Sequence Label: FLAG-Pol-II CC
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      654..663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctactaga gaacccactg ccttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctaggac catggactac aaggacgacg atgacaaggc tagcatgcac
      121 gggggtggcc ccccctcggg ggacagcgca tgcccgctgc gcaccatcaa gagagtccag
      181 ttcggagtcc tgagtccgga tgaactgaag cgaatgtctg tgacggaggg tggcatcaaa
      241 tacccagaga cgactgaggg aggccgcccc aagcttgggg ggctgatgga cccgaggcag
      301 ggggtgattg agcggactgg ccgctgccaa acatgtgcag gaaacatgac agagtgtcct
      361 ggccactttg gccacattga actggccaag cctgtgtttc acgtgggctt cctggtgaag
      421 acaatgaaag ttttgcgctg tgtctgcttc ttctgctcca aactgcttgt ggactctaac
      481 aacccaaaga tcaaggatat cctggctaag tccaagggac agcccaagaa gcggctcaca
      541 catgtctacg acctttgcaa gggcaaaaac atatgcgagg gtggggagga gatggacaac
      601 aagttcggtg tggaacaacc tgagggtgac gaggatctga ccaaagaaaa gggccatggt
      661 ggctgtgggc ggtaccagcc caggatccgg cgttctggcc tagagctgta tgcggaatgg
      721 aagcacgtta atgaggactc tcaggagaag aagatcctgc tgagtccaga gcgagtgcat
      781 gagatcttca aacgcatctc agatgaggag tgttttg
//
