LOCUS       sequence-44578-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: zgc:162148_R (OZ580)
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      451..809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(538..557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     748..769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatccaag gctttggcgg ccgctgacta caaagacgat gatgacaaat ctagacccgg
       61 ggagcgcccc ttccagtgtc gcatttgcat gcggaacttt tcggacgaag caaacttgcg
      121 tcgtcatacc cgtactcata ccggtgaaaa accgtttcag tgtcggatct gtatgcgaaa
      181 tttctcccag cagaccaact tgacccgtca tctacgtacg cacaccggcg agaagccatt
      241 ccaatgccga atatgcatgc gcaacttcag tgttggttct aacttgaccc gtcacctaaa
      301 aacccacctg aggggatcct ctgcacagta gtaactagag actagaaaaa ggccgacaag
      361 tcccgctccg ctgaagatcc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      421 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      661 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      721 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      781 accctatctc ggtctattct tttgatttat aagggattt
//
