LOCUS       sequence-44581-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: cx52.9_R (OZ584)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..48
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      450..808
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(537..556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     747..768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatccaag ctttggcggc cgctgactac aaagacgatg atgacaaatc tagacccggg
       61 gagcgcccct tccagtgtcg catttgcatg cggaactttt cgaccaagca gggccttcag
      121 cgccataccc gtactcatac cggtgaaaaa ccgtttcagt gtcggatctg tatgcgaaat
      181 ttctccatga gccaccactt gatccggcat ctacgtacgc acaccggcga gaagccattc
      241 caatgccgaa tatgcatgcg caacttcagt ctcgggcaca ccctgaaccg ccacctaaaa
      301 acccacctga ggggatcctc tgcacagtag taactagaga ctagaaaaag gccgacaagt
      361 cccgctccgc tgaagatcct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      421 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      481 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      541 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      601 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      661 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      721 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      781 ccctatctcg gtctattctt ttgatttata agggattt
//
