LOCUS       sequence-445869-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac expression vector with integrin beta1 tagged with mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pPB.DEST-ITGB1(WT)-mRuby2
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1676..2692
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2616..2638
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2700..2719
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5256..5966
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     protein_bind    complement(5987..6011)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6044..6061)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6050..6274
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6321..6337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6345..6361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6369..6375,6376..6393,6394..6399))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6414..6435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6701..6763
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6814..6834)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6814..6832)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6853..6869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6877..6893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6901..6907,6908..6925,6926..6931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6946..6967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7084..7101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7255..7843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7335..7354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8014..8805,8806..8874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8637..8656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8875..8979)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggggggccc gggggggggg gggggggggg gggggggggg gggggggggg
     1561 ggcggggcgg agaggtgcgg cggcagccaa ccagagcggc gcgctccgaa agtttccttt
     1621 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     1681 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     1741 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     1801 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     1861 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     1921 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     1981 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     2041 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     2101 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     2161 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     2221 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     2281 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     2341 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     2401 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     2461 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     2521 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     2581 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     2641 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     2701 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcagat ctgcggccgc
     2761 ctcgagaagc ttgatcaaca agtttgtaca aaaaaagcag gctggcgccg gaaccaattc
     2821 agtcgactgg atccggtacc accgccatga atttacaacc aattttctgg attggactga
     2881 tcagttcagt ttgctgtgtg tttgctcaaa cagatgaaaa tagatgttta aaagcaaatg
     2941 ccaaatcatg tggagaatgt atacaagcag ggccaaattg tgggtggtgc acaaattcaa
     3001 catttttaca ggaaggaatg cctacttctg cacgatgtga tgatttagaa gccttaaaaa
     3061 agaagggttg ccctccagat gacatagaaa atcccagagg ctccaaagat ataaagaaaa
     3121 ataaaaatgt aaccaaccgt agcaaaggaa cagcagagaa gctcaagcca gaggatatta
     3181 ctcagatcca accacagcag ttggttttgc gattaagatc aggggagcca cagacattta
     3241 cattaaaatt caagagagct gaagactatc ccattgacct ctactacctt atggacctgt
     3301 cttactcaat gaaagacgat ttggagaatg taaaaagtct tggaacagat ctgatgaatg
     3361 aaatgaggag gattacttcg gacttcagaa ttggatttgg ctcatttgtg gaaaagactg
     3421 tgatgcctta cattagcaca acaccagcta agctcaggaa cccttgcaca agtgaacaga
     3481 actgcaccag cccatttagc tacaaaaatg tgctcagtct tactaataaa ggagaagtat
     3541 ttaatgaact tgttggaaaa cagcgcatat ctggaaattt ggattctcca gaaggtggtt
     3601 tcgatgccat catgcaagtt gcagtttgtg gatcactgat tggctggagg aatgttacac
     3661 ggctgctggt gttttccaca gatgccgggt ttcactttgc tggagatggg aaacttggtg
     3721 gcattgtttt accaaatgat ggacaatgtc acctggaaaa taatatgtac acaatgagcc
     3781 attattatga ttatccttct attgctcacc ttgtccagaa actgagtgaa aataatattc
     3841 agacaatttt tgcagttact gaagaatttc agcctgttta caaggagctg aaaaacttga
     3901 tccctaagtc agcagtagga acattatctg caaattctag caatgtaatt cagttgatca
     3961 ttgatgcata caattccctt tcctcagaag tcattttgga aaacggcaaa ttgtcagaag
     4021 gagtaacaat aagttacaaa tcttactgca agaacggggt gaatggaaca ggggaaaatg
     4081 gaagaaaatg ttccaatatt tccattggag atgaggttca atttgaaatt agcataactt
     4141 caaataagtg tccaaaaaag gattctgaca gctttaaaat taggcctctg ggctttacgg
     4201 aggaagtaga ggttattctt cagtacatct gtgaatgtga atgccaaagc gaaggcatcc
     4261 ctgaaagtcc caagtgtcat gaaggaaatg ggacatttga gtgtggcgcg tgcaggtgca
     4321 atgaagggcg tgttggtaga cattgtgaat gcagcacaga tgaagttaac agtgaagaca
     4381 tggatgctta ctgcaggaaa gaaaacagtt cagaaatctg cagtaacaat ggagagtgcg
     4441 tctgcggaca gtgtgtttgt aggaagaggg ataatacaaa tgaaatttat tctggcaaat
     4501 tctgcgagtg tgataatttc aactgtgata gatccaatgg cttaatttgt ggaggaaatg
     4561 gtgtttgcaa gtgtcgtgtg tgtgagtgca accccaacta cactggcagt gcatgtgact
     4621 gttctttgga tactagtact tgtgaagcca gcaacggaca gatctgcaat ggccggggca
     4681 tctgcgagtg tggtgtctgt aagtgtacag atccgaagtt tcaagggcaa acgtgtgaga
     4741 tgtgtcagac ctgccttggt gtctgtgctg agcataaaga atgtgttcag tgcagagcct
     4801 tcaataaagg agaaaagaaa gacacatgca cacaggaatg ttcctatttt aacattacca
     4861 aggtagaaag tcgggacaaa ttaccccagc cggtccaacc tgatcctgtg tcccattgta
     4921 aggagaagga tgttgacgac tgttggttct attttacgta ttcagtgaat gggaacaacg
     4981 aggtcatggt tcatgttgtg gagaatccag agtgtcccac tggtccagac atcattccaa
     5041 ttgtagctgg tgtggttgct ggaattgttc ttattggcct tgcgttactc ctcatttgga
     5101 agcttttaat gataattcat gacagaaggg agtttgctaa atttgaaaag gagaaaatga
     5161 atgccaaatg ggacacgggt gaaaatccta tttataagag tgccgtaaca actgtggtca
     5221 atccgaagta tgagggaaaa ggcggcggcg gccgcatggt gtctaagggc gaagagctga
     5281 tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc caccaattca
     5341 aatgcacagg tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg aggatcaaag
     5401 tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg ttcatgtatg
     5461 gcagccgtac ttttatcaag tacccgaaag gcattcctga tttctttaaa cagtcctttc
     5521 ctgagggttt tacttgggaa agagttacga gatacgaaga tggtggagtc gtcaccgtca
     5581 tgcaggacac cagccttgag gatggctgtc tcgtttacca cgtccaagtc agaggggtaa
     5641 actttccctc caatggtccc gtgatgcaga agaagaccaa gggttgggag cctaatacag
     5701 agatgatgta tccagcagat ggtggtctga ggggatacac tcatatggca ctgaaagttg
     5761 atggtggtgg ccatctgtct tgctctttcg taacaactta caggtcaaaa aagaccgtcg
     5821 ggaacatcaa gatgcccggt atccatgccg ttgatcaccg cctggaaagg ttagaggaaa
     5881 gtgacaatga aatgttcgta gtacaacgcg aacacgcagt tgccaagttc gccgggcttg
     5941 gtggtgggat ggacgagctg tacaagtagt aactcgagat atctagaccc agctttcttg
     6001 tacaaagtgg ttgatcatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct
     6061 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6121 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6181 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6241 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc
     6301 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6481 tgccagcgga tccattcatg aatgaattca tgtcgacata ctagttaaaa gttttgttac
     6541 tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat
     6601 tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc
     6661 aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat
     6721 gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct agtatacgcg
     6781 tatgcggccg cttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     6841 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6901 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6961 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7021 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7081 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7141 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7201 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7261 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7321 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7381 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7441 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7501 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7561 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7621 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7681 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7741 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7801 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7861 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7921 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7981 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8041 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8101 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8161 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8221 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8281 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8341 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8401 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8461 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8521 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8581 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8641 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8701 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8761 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8821 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8881 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8941 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9001 ac
//
