LOCUS       sequence-445873-        8122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB and GvpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBR
FEATURES             Location/Qualifiers
     source          1..8122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..31)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     172..194
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(188..379)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     616..635
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        616..634
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2381..2398
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6141..6159)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6155..6202
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6269..6360
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7225..7264
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7295..7883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7784..7803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8037..8054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(8069..8122,1..86)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
       61 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      121 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      181 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      241 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      301 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      361 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      421 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      481 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      541 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      601 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      661 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      721 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
      781 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
      841 tttaaaaaca atgggatcat caggaggaac gtaaaaatga ccgtcttaac agacaaaagg
      901 aaaaaaggca gtggagcttt tatacaagat gacgagacaa aagaggttct ttcaagagcg
      961 ctgagctatt taaaatccgg ctattccatt cattttacag gtcctgccgg cggaggcaaa
     1021 acctctttag cgcgagcgct tgctaaaaag agaaagcgtc ctgtaatgct gatgcacggg
     1081 aatcacgagc tcaacaacaa agatttaatt ggcgatttta cgggatacac gagcaaaaaa
     1141 gtaatcgacc agtacgttcg ttctgtctat aaaaaagatg aacaggtgag tgaaaactgg
     1201 caggatggcc gattgcttga agctgtaaaa aatggctata cgctgattta cgacgaattt
     1261 actcgttcta agcctgcgac gaataatatc tttctatcga tattagaaga aggcgtgctg
     1321 ccgctgtatg gagtaaaaat gaccgatcct tttgtgcgcg tgcatcccga tttccgcgtc
     1381 atcttcacaa gcaatccagc tgagtatgcc ggcgtatatg atacgcaaga tgcgcttctc
     1441 gacaggttaa ttaccatgtt tattgattat aaagacatcg acagagagac agcgatttta
     1501 acggagaaaa cggacgtaga agaagatgaa gcgcgcacaa ttgtaacgct cgtagcaaac
     1561 gtgcgaaacc gctctggaga cgaaaacagc agcggactta gcctgcgggc ttcgcttatg
     1621 atcgctaccc ttgccacgca gcaagacatt cctatcgatg gaagtgacga agattttcaa
     1681 acgttatgta tcgatatttt gcatcatccg cttaccaaat gtttggatga agaaaatgca
     1741 aaaagcaaag ccgaaaaaat cattttagaa gaatgtaaga atatagacac tgaagaaaag
     1801 taaaggagct tgaaaacatg agtgaaacaa acgaaacagg tatttatatt tttagcgcca
     1861 ttcaaacgga taaagacgaa gaatttggcg ccgtggaagt agaaggaaca aaagctgaaa
     1921 catttttgat tcgctacaaa gacgcggcta tggtagcagc tgaagtaccg atgaaaattt
     1981 atcatcctaa tcgccaaaat ttattaatgc atcaaaacgc agtagcagcg attatggaca
     2041 agaacgatac ggttattcca atcagctttg ggaatgtatt caaatcaaaa gaagacgtaa
     2101 aagttctttt ggaaaacctt tatccgcagt ttgaaaagct gtttccagcg atcaaaggaa
     2161 aaattgaagt cggtttaaaa gtaattggga aaaaagaatg gcttgagaaa aaagtaaacg
     2221 aaaatcctga acttgagaaa gtatcagcat ccgtaaaagg aaaatcagaa gcagccggtt
     2281 attatgagcg tattcaactt ggaggaatgg ctcaaaagat gtttacttcc ctgcaaaaag
     2341 aagtcaagac agatgtgttt tctccgcttg aagaagcagc ggaagcagca aaagcaaatg
     2401 agccaacggg cgaaacgatg cttttaaacg cgtctttctt aattaaccga gaagatgaag
     2461 cgaagtttga tgaaaaagta aatgaagcgc atgaaaactg gaaagacaaa gccgattttc
     2521 attacagcgg tccttggcct gcttataatt ttgtgaacat tcgcctaaaa gtagaagaga
     2581 aataacgtgc ttcacaaatt agtaaccgca cccattaacc ttgtagtgaa aatcggcgaa
     2641 aaagtacagg aagaagctga taaacagcta tatgaccttc cgacgattca gcaaaagctc
     2701 attcagcttc aaatgatgtt tgagcttggt gaaattccag aagaagcgtt tcaagaaaaa
     2761 gaagatgaat tgttaatgag gtacgaaatt gcgaaacgca gagaaattga acaatgggaa
     2821 gagctaacac aaaaaagaaa tgaggaatcc tagatgggag aattactgta tttatacggt
     2881 ttaattccaa caaaagaagc agcagccata gagccgtttc catcttataa ggggtttgac
     2941 ggagaacatt cactgtaccc aattgcgttt gatcaggtga cggctgtagt ttctaagctg
     3001 gatgctgaca cctattcaga aaaagtgatt caagaaaaaa tggagcagga tatgagctgg
     3061 ctgcaggaaa aagcatttca tcatcacgaa acggtagccg ctttgtacga agaatttacg
     3121 atcattccat taaaattttg caccatttat aaaggtgaag aaagtctgca ggcagctatt
     3181 gagattaaca aagaaaagat agagaattca ctgacgctgc ttcaaggaaa tgaagagtgg
     3241 aatgtgaaaa tttactgtga tgatacagag cttaaaaaag gaatcagcga aacgaatgaa
     3301 agcgtgaaag cgaaaaaaca agaaattagt cacttatcac caggaagaca gttttttgaa
     3361 aagaaaaaaa tagatcagct gattgaaaaa gaattagagc ttcacaaaaa caaagtgtgt
     3421 gaagagatac atgacaagct aaaagaatta tcgctttatg actctgttaa aaagaattgg
     3481 agcaaagacg taactggcgc agctgaacag atggcgtgga acagcgtgtt tcttctcccg
     3541 tctctgcaga ttactaagtt cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat
     3601 aaaggctgga agtttgaagt gacgggacca tggccgccct atcatttctc gagctttgcg
     3661 taaagtgagg aattaacatt atgtctctta aacaatccat ggagaataaa gatattgctc
     3721 ttattgatat tttagatgtc attttagata aaggagtcgc cattaaagga gacttaatca
     3781 tttccatagc tggcgtcgat ttagtgtatt tggatttgcg ggtgcttatt tcttcggttg
     3841 aaacgcttgt gcaagcaaaa gaaggaaatc acaaaccaat cacttctgaa caatttgata
     3901 aacaaaagga ggaattaatg gatgcaaccg gtcagccaag caaatggacg aatccacttg
     3961 gatcctgatc aagctgaaca aggcttagcg cagcttgtga tgacagttat tgagctattg
     4021 aggcaaatcg ttgaacgtca tgccatgagg cgggtggagg gtggaacgtt gacggacgaa
     4081 caaattgaaa acttaggaat tgcactaatg aacttagaag aaaaaatgga cgagttgaaa
     4141 gaggtgttcg gtctggatgc agaagattta aatattgatc ttggaccgct aggcagcctg
     4201 ctttaagcgg tcagtaggag gaacagtatg gcagtcgaac ataatatgca gtcaagtacg
     4261 attgtagatg tgctcgaaaa gattttggat aaaggagtcg ttatagcggg ggacatcacc
     4321 gtaggaattg cagatgtcga gctattaacg ataaagatcc gcttgattgt ggcttcggtt
     4381 gataaggcaa aagaaatcgg catggactgg tgggaaaatg atccgtatct cagttcaaaa
     4441 ggagccaata acaaagcgct cgaagaagaa aataaaatgc tgcatgagcg gttaaaaacg
     4501 cttgaagaaa aaatagaaac gaaacgttaa aaactgtacg ctacttaaaa aatggaggga
     4561 tttacaatgg caactgaaac aaaattagat aacacacagg cagaaaacaa ggaaaataaa
     4621 aatgcggaaa acggttcaaa agaaaagaac ggttcaaaag caagcaaaac aacaagcagc
     4681 gggccaatca aacgagcggt agcaggaggc atcatcggtg caacgattgg atatgtatcg
     4741 actcctgaaa atcgaaaaag tctccttgac cgcattgata cagacgaatt aaaaagcaaa
     4801 gcatctgatt taggaacaaa ggtaaaagaa aaatcaaaaa gcagcgtggc cagcctgaaa
     4861 acatctgcgg gaagcttgtt taaaaaagat aaagataaat caaaagatga tgaagaaaac
     4921 gtaaattctt ctagtagcga aacagaagac gataacgttc aagagtacga cgagttaaaa
     4981 gaagaaaatc aaactcttca agatcgctta tcacagcttg aagaaaaaat gaacatgctt
     5041 gttgagctta gcctcaataa aaatcaagac gaagaagcgg aagatacaga ttccgacgaa
     5101 gaagagaacg atgagaacga tgaaaacgat gaaaacgagc aggacgatga aaacgaagaa
     5161 gaaacatcta agccacgtaa aaaggataaa aaagaagctg aggaagaaga aagtgaaagt
     5221 gacgaagaca gcgaggaaga agaggaagat tctcgctcaa acaaaaaaaa taaaaaagta
     5281 aaaacagaag aagaagacga agatgaaagc gaagaagaaa aaaaggaagc gaaaccaaaa
     5341 aagtcaacag ctaaaaaatc aaaaaataca aaagcaaaga aaaacacgga cgaagaagat
     5401 gatgaagcaa catctctttc tagtgaagac gatacaacag cctaagacgt aaaggaggaa
     5461 agaaagacat gagtacaggc ccttcttttt caactaaaga caatacgctt gaatactttg
     5521 tgaaagcttc taataaacac ggcttttcac ttgatatttc attaaatgta aacggcgctg
     5581 tgatttccgg taccatgatt tcagcaaaag aatactttga ttacttaagc gaaacgtttg
     5641 aagaaggcag tgaagtggct caggcgctaa gcgaacaatt ctctttagca agcgaagcga
     5701 gcgaatcaaa cggagaagca gaagcccatt ttattcattt gaaaaataca aagatttact
     5761 gtggagacag taaatctact ccttctaaag gcaaaatctt ttggagaggg aaaatagcag
     5821 aagtagacgg gtttttctta ggaaagattt ctgatgcaaa atcaacgagt aaaaagagtt
     5881 cataaaaaac ggcggggtga ttgccccgcc gttttttagt gatgtgatga gatgtgcagc
     5941 ttcttttttc gatacatgag cggagtcatc ttcatttctt ttcgaaactg attaataaaa
     6001 tagcttgtac tattaaaccc tacttgatag gcgacttccg tcacatttgc ttctgcttgc
     6061 tgcagtccgg attcgcgaac gcgtctcgag agatccggct gctaacaaag cccgaaagga
     6121 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     6181 acgggtcttg aggggttttt tgctgaaagg aggaactata tccgactagt agcccgccta
     6241 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
     6301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6421 gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca
     6481 cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct
     6541 gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc
     6601 cgtgtagatg cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg
     6661 gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta
     6721 tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc
     6781 ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt
     6841 gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg
     6901 cctgcggcaa tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct
     6961 tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt
     7021 tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca
     7081 cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac
     7141 acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct
     7201 tcactgatta agcattggta aaccgataca attaaaggct ccttttggag cctttttttt
     7261 tggacggacc ggtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7441 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7981 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8041 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     8101 gcggtatttc acaccgcaat gg
//
