LOCUS       sequence-445883-        4686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with integrin beta1 NPxY mutant (YYFF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pENTR2b-ITGB1(YYFF)
FEATURES             Location/Qualifiers
     source          1..4686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2915..3014)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3126..3145)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3137..3946
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3204..3223)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4036..4624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4525..4544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccgg taccaccgcc atgaatttac aaccaatttt ctggattgga ctgatcagtt
      541 cagtttgctg tgtgtttgct caaacagatg aaaatagatg tttaaaagca aatgccaaat
      601 catgtggaga atgtatacaa gcagggccaa attgtgggtg gtgcacaaat tcaacatttt
      661 tacaggaagg aatgcctact tctgcacgat gtgatgattt agaagcctta aaaaagaagg
      721 gttgccctcc agatgacata gaaaatccca gaggctccaa agatataaag aaaaataaaa
      781 atgtaaccaa ccgtagcaaa ggaacagcag agaagctcaa gccagaggat attactcaga
      841 tccaaccaca gcagttggtt ttgcgattaa gatcagggga gccacagaca tttacattaa
      901 aattcaagag agctgaagac tatcccattg acctctacta ccttatggac ctgtcttact
      961 caatgaaaga cgatttggag aatgtaaaaa gtcttggaac agatctgatg aatgaaatga
     1021 ggaggattac ttcggacttc agaattggat ttggctcatt tgtggaaaag actgtgatgc
     1081 cttacattag cacaacacca gctaagctca ggaacccttg cacaagtgaa cagaactgca
     1141 ccagcccatt tagctacaaa aatgtgctca gtcttactaa taaaggagaa gtatttaatg
     1201 aacttgttgg aaaacagcgc atatctggaa atttggattc tccagaaggt ggtttcgatg
     1261 ccatcatgca agttgcagtt tgtggatcac tgattggctg gaggaatgtt acacggctgc
     1321 tggtgttttc cacagatgcc gggtttcact ttgctggaga tgggaaactt ggtggcattg
     1381 ttttaccaaa tgatggacaa tgtcacctgg aaaataatat gtacacaatg agccattatt
     1441 atgattatcc ttctattgct caccttgtcc agaaactgag tgaaaataat attcagacaa
     1501 tttttgcagt tactgaagaa tttcagcctg tttacaagga gctgaaaaac ttgatcccta
     1561 agtcagcagt aggaacatta tctgcaaatt ctagcaatgt aattcagttg atcattgatg
     1621 catacaattc cctttcctca gaagtcattt tggaaaacgg caaattgtca gaaggagtaa
     1681 caataagtta caaatcttac tgcaagaacg gggtgaatgg aacaggggaa aatggaagaa
     1741 aatgttccaa tatttccatt ggagatgagg ttcaatttga aattagcata acttcaaata
     1801 agtgtccaaa aaaggattct gacagcttta aaattaggcc tctgggcttt acggaggaag
     1861 tagaggttat tcttcagtac atctgtgaat gtgaatgcca aagcgaaggc atccctgaaa
     1921 gtcccaagtg tcatgaagga aatgggacat ttgagtgtgg cgcgtgcagg tgcaatgaag
     1981 ggcgtgttgg tagacattgt gaatgcagca cagatgaagt taacagtgaa gacatggatg
     2041 cttactgcag gaaagaaaac agttcagaaa tctgcagtaa caatggagag tgcgtctgcg
     2101 gacagtgtgt ttgtaggaag agggataata caaatgaaat ttattctggc aaattctgcg
     2161 agtgtgataa tttcaactgt gatagatcca atggcttaat ttgtggagga aatggtgttt
     2221 gcaagtgtcg tgtgtgtgag tgcaacccca actacactgg cagtgcatgt gactgttctt
     2281 tggatactag tacttgtgaa gccagcaacg gacagatctg caatggccgg ggcatctgcg
     2341 agtgtggtgt ctgtaagtgt acagatccga agtttcaagg gcaaacgtgt gagatgtgtc
     2401 agacctgcct tggtgtctgt gctgagcata aagaatgtgt tcagtgcaga gccttcaata
     2461 aaggagaaaa gaaagacaca tgcacacagg aatgttccta ttttaacatt accaaggtag
     2521 aaagtcggga caaattaccc cagccggtcc aacctgatcc tgtgtcccat tgtaaggaga
     2581 aggatgttga cgactgttgg ttctatttta cgtattcagt gaatgggaac aacgaggtca
     2641 tggttcatgt tgtggagaat ccagagtgtc ccactggtcc agacatcatt ccaattgtag
     2701 ctggtgtggt tgctggaatt gttcttattg gccttgcgtt actcctcatt tggaagcttt
     2761 taatgataat tcatgacaga agggagtttg ctaaatttga aaaggagaaa atgaatgcca
     2821 aatgggacac gggtgaaaat cctattttta agagtgccgt aacaactgtg gtcaatccga
     2881 agtttgaggg aaaatagtga ctcgagatat ctagacccag ctttcttgta caaagttggc
     2941 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     3001 aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta
     3061 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3121 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3181 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3241 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3301 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3361 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3421 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3481 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3541 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3601 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3661 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3721 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3781 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3841 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3901 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3961 ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc ccgtagaaaa
     4021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4681 catgtt
//
