LOCUS       sequence-445888-        5002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector with Dok1 tagged with the BiFC V2 fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pDEST-Dok1-V2
FEATURES             Location/Qualifiers
     source          1..5002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    942..966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2413..2437)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2660..2681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2758..2775)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2764..2988
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3076..3093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3247..3835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3327..3346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4006..4797,4798..4866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4629..4648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4867..4971)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatattacg gggtactcga gacaagtttg tacaaaaaag
      961 ttggcaccat ggacggagca gtgatggaag ggccgctttt tttgcagagt cagcgctttg
     1021 ggaccaagag gtggaggaag acctgggccg tgctctaccc ggccagtccc cacggcgtag
     1081 cgcggctcga gttctttgac cataaggggt cgagctctgg gggtggccga gggagctcgc
     1141 gccgcctgga ctgcaaagtg atccgtctgg ctgagtgtgt gagtgtggcc cccgtcaccg
     1201 tggagacccc ccctgagccc ggcgccactg ccttccgcct ggacactgct cagcgctcgc
     1261 acctgctggc ggccgacgcg ccgtccagtg cagcctgggt gcagacgctg tgccgaaacg
     1321 cctttccgaa aggcagctgg actctggcgc ctaccgataa cccacctaag ctttctgccc
     1381 tggagatgct ggagaactcc ttgtacagcc ctacctggga aggatcccaa ttctgggtaa
     1441 cggtgcagag gactgaggcc gccgagcgct gtggcctgca tggctcctac gtgctgaggg
     1501 tggaggctga aaggctgact ctcctgaccg tgggggccca gagtcagata ctggagccac
     1561 tcctgtcctg gccctacact ctgttgcgtc gctatggccg ggacaaggtc atgttctctt
     1621 tcgaggccgg ccgccgctgc ccctcaggcc ctggaacctt caccttccag acggcacagg
     1681 gaaatgacat cttccaggca gttgagactg ccatccaccg gcagaaggcc cagggaaagg
     1741 ccggacaggg gcacgatgtt ctcagagctg actcccatga aggggaggtg gcagagggga
     1801 agttgccttc cccacctggc ccccaagagc tcctcgacag tcccccagcc ctgtatgctg
     1861 agcccttaga ctccctgcgc attgctccat gcccttccca ggactcccta tactcagacc
     1921 ccttggacag cacgtctgct caggcaggag agggagtaca acggaagaaa cctctctatt
     1981 gggacttgta tgagcatgcg cagcagcagt tgctgaaggc caagctgaca gaccccaaag
     2041 aggatcccat ctatgatgaa cctgagggcc tggccccagt ccctccccag ggcctttatg
     2101 atctgcctcg ggagcccaag gatgcatggt ggtgccaagc tcgggtgaag gaggagggct
     2161 atgagctccc ctacaaccct gccactgatg actacgctgt gccaccccct cggagcacaa
     2221 agcccctcct tgctcccaag ccccagggcc cagccttccc tgaacctggt actgcaactg
     2281 gcagtggcat caaaagccac aactcagccc tgtacagcca ggtccagaag agcggggcct
     2341 cagggagctg ggactgtggg ctctctagag tagggactga caagactggg gtcaagtcag
     2401 agggctctac cttgccaact ttcttgtaca aagtggtcat cgatggtggc ggtggctctg
     2461 gaggtggtgg gtcctccgga aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     2521 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2581 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     2641 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2701 tcggcatgga cgagctgtac aagtaatcta gagggcccgt ttaaacccgc tgatcagcct
     2761 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2821 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2881 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2941 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3001 aaagaaccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3061 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3661 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3901 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3961 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4021 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4081 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4141 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4201 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4261 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4321 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4381 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4441 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4501 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4561 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4621 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4681 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4741 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4801 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4861 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4921 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4981 ccgaaaagtg ccacctgacg tc
//
