LOCUS       sequence-445892-        7485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB and GvpN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBN
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    138..277
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(203..222)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     363..385
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(379..570)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     807..826
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        807..825
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1935..1952
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5695..5713)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5709..5756
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5823..5914
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6779..6818
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6849..7437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7338..7357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
       61 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      121 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
      181 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
      241 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
      301 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
      361 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
      421 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
      481 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
      541 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
      601 catggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
      661 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
      721 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
      781 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
      841 tccctctaga gatagcttag cgatgtgaaa gaagctggag aaaactttca agaaaaagtt
      901 tcttcttcag acgataaaga gaagtcaaaa aataagcgga aaataaaagg cgtaaatcag
      961 attaaaaaag caacggatat taaaagctct actaagatta aaagttctaa tgatattaaa
     1021 tcatctacag atttaaaaac aatgggatca ttagatctaa ctattggagg ctactaaaaa
     1081 tggaaattaa aaaaattatg caagccgtga acgacttttt cggtgaacac gtagctcctc
     1141 ctcataaaat tacctcggtg gaagctactg aagatgaagg ttggagagtt attgttgaag
     1201 tcattgaaga acgagaatat atgaaaaaat acgccaaaga tgaaatgctc ggaacgtacg
     1261 agtgctttgt aaataaagaa aaagaagtca tttcattcaa acgactcgac gtcagatata
     1321 gaagcgccat tggcattgaa gcataaacga taagatgggg agcttgaaaa catgagtgaa
     1381 acaaacgaaa caggtattta tatttttagc gccattcaaa cggataaaga cgaagaattt
     1441 ggcgccgtgg aagtagaagg aacaaaagct gaaacatttt tgattcgcta caaagacgcg
     1501 gctatggtag cagctgaagt accgatgaaa atttatcatc ctaatcgcca aaatttatta
     1561 atgcatcaaa acgcagtagc agcgattatg gacaagaacg atacggttat tccaatcagc
     1621 tttgggaatg tattcaaatc aaaagaagac gtaaaagttc ttttggaaaa cctttatccg
     1681 cagtttgaaa agctgtttcc agcgatcaaa ggaaaaattg aagtcggttt aaaagtaatt
     1741 gggaaaaaag aatggcttga gaaaaaagta aacgaaaatc ctgaacttga gaaagtatca
     1801 gcatccgtaa aaggaaaatc agaagcagcc ggttattatg agcgtattca acttggagga
     1861 atggctcaaa agatgtttac ttccctgcaa aaagaagtca agacagatgt gttttctccg
     1921 cttgaagaag cagcggaagc agcaaaagca aatgagccaa cgggcgaaac gatgctttta
     1981 aacgcgtctt tcttaattaa ccgagaagat gaagcgaagt ttgatgaaaa agtaaatgaa
     2041 gcgcatgaaa actggaaaga caaagccgat tttcattaca gcggtccttg gcctgcttat
     2101 aattttgtga acattcgcct aaaagtagaa gagaaataac gtgcttcaca aattagtaac
     2161 cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta caggaagaag ctgataaaca
     2221 gctatatgac cttccgacga ttcagcaaaa gctcattcag cttcaaatga tgtttgagct
     2281 tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat gaattgttaa tgaggtacga
     2341 aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta acacaaaaaa gaaatgagga
     2401 atcctagatg ggagaattac tgtatttata cggtttaatt ccaacaaaag aagcagcagc
     2461 catagagccg tttccatctt ataaggggtt tgacggagaa cattcactgt acccaattgc
     2521 gtttgatcag gtgacggctg tagtttctaa gctggatgct gacacctatt cagaaaaagt
     2581 gattcaagaa aaaatggagc aggatatgag ctggctgcag gaaaaagcat ttcatcatca
     2641 cgaaacggta gccgctttgt acgaagaatt tacgatcatt ccattaaaat tttgcaccat
     2701 ttataaaggt gaagaaagtc tgcaggcagc tattgagatt aacaaagaaa agatagagaa
     2761 ttcactgacg ctgcttcaag gaaatgaaga gtggaatgtg aaaatttact gtgatgatac
     2821 agagcttaaa aaaggaatca gcgaaacgaa tgaaagcgtg aaagcgaaaa aacaagaaat
     2881 tagtcactta tcaccaggaa gacagttttt tgaaaagaaa aaaatagatc agctgattga
     2941 aaaagaatta gagcttcaca aaaacaaagt gtgtgaagag atacatgaca agctaaaaga
     3001 attatcgctt tatgactctg ttaaaaagaa ttggagcaaa gacgtaactg gcgcagctga
     3061 acagatggcg tggaacagcg tgtttcttct cccgtctctg cagattacta agttcgtaaa
     3121 cgaaatagaa gagcttcagc aaaggcttga aaataaaggc tggaagtttg aagtgacggg
     3181 accatggccg ccctatcatt tctcgagctt tgcgtaaagt gaggaattaa cattatgtct
     3241 cttaaacaat ccatggagaa taaagatatt gctcttattg atattttaga tgtcatttta
     3301 gataaaggag tcgccattaa aggagactta atcatttcca tagctggcgt cgatttagtg
     3361 tatttggatt tgcgggtgct tatttcttcg gttgaaacgc ttgtgcaagc aaaagaagga
     3421 aatcacaaac caatcacttc tgaacaattt gataaacaaa aggaggaatt aatggatgca
     3481 accggtcagc caagcaaatg gacgaatcca cttggatcct gatcaagctg aacaaggctt
     3541 agcgcagctt gtgatgacag ttattgagct attgaggcaa atcgttgaac gtcatgccat
     3601 gaggcgggtg gagggtggaa cgttgacgga cgaacaaatt gaaaacttag gaattgcact
     3661 aatgaactta gaagaaaaaa tggacgagtt gaaagaggtg ttcggtctgg atgcagaaga
     3721 tttaaatatt gatcttggac cgctaggcag cctgctttaa gcggtcagta ggaggaacag
     3781 tatggcagtc gaacataata tgcagtcaag tacgattgta gatgtgctcg aaaagatttt
     3841 ggataaagga gtcgttatag cgggggacat caccgtagga attgcagatg tcgagctatt
     3901 aacgataaag atccgcttga ttgtggcttc ggttgataag gcaaaagaaa tcggcatgga
     3961 ctggtgggaa aatgatccgt atctcagttc aaaaggagcc aataacaaag cgctcgaaga
     4021 agaaaataaa atgctgcatg agcggttaaa aacgcttgaa gaaaaaatag aaacgaaacg
     4081 ttaaaaactg tacgctactt aaaaaatgga gggatttaca atggcaactg aaacaaaatt
     4141 agataacaca caggcagaaa acaaggaaaa taaaaatgcg gaaaacggtt caaaagaaaa
     4201 gaacggttca aaagcaagca aaacaacaag cagcgggcca atcaaacgag cggtagcagg
     4261 aggcatcatc ggtgcaacga ttggatatgt atcgactcct gaaaatcgaa aaagtctcct
     4321 tgaccgcatt gatacagacg aattaaaaag caaagcatct gatttaggaa caaaggtaaa
     4381 agaaaaatca aaaagcagcg tggccagcct gaaaacatct gcgggaagct tgtttaaaaa
     4441 agataaagat aaatcaaaag atgatgaaga aaacgtaaat tcttctagta gcgaaacaga
     4501 agacgataac gttcaagagt acgacgagtt aaaagaagaa aatcaaactc ttcaagatcg
     4561 cttatcacag cttgaagaaa aaatgaacat gcttgttgag cttagcctca ataaaaatca
     4621 agacgaagaa gcggaagata cagattccga cgaagaagag aacgatgaga acgatgaaaa
     4681 cgatgaaaac gagcaggacg atgaaaacga agaagaaaca tctaagccac gtaaaaagga
     4741 taaaaaagaa gctgaggaag aagaaagtga aagtgacgaa gacagcgagg aagaagagga
     4801 agattctcgc tcaaacaaaa aaaataaaaa agtaaaaaca gaagaagaag acgaagatga
     4861 aagcgaagaa gaaaaaaagg aagcgaaacc aaaaaagtca acagctaaaa aatcaaaaaa
     4921 tacaaaagca aagaaaaaca cggacgaaga agatgatgaa gcaacatctc tttctagtga
     4981 agacgataca acagcctaag acgtaaagga ggaaagaaag acatgagtac aggcccttct
     5041 ttttcaacta aagacaatac gcttgaatac tttgtgaaag cttctaataa acacggcttt
     5101 tcacttgata tttcattaaa tgtaaacggc gctgtgattt ccggtaccat gatttcagca
     5161 aaagaatact ttgattactt aagcgaaacg tttgaagaag gcagtgaagt ggctcaggcg
     5221 ctaagcgaac aattctcttt agcaagcgaa gcgagcgaat caaacggaga agcagaagcc
     5281 cattttattc atttgaaaaa tacaaagatt tactgtggag acagtaaatc tactccttct
     5341 aaaggcaaaa tcttttggag agggaaaata gcagaagtag acgggttttt cttaggaaag
     5401 atttctgatg caaaatcaac gagtaaaaag agttcataaa aaacggcggg gtgattgccc
     5461 cgccgttttt tagtgatgtg atgagatgtg cagcttcttt tttcgataca tgagcggagt
     5521 catcttcatt tcttttcgaa actgattaat aaaatagctt gtactattaa accctacttg
     5581 ataggcgact tccgtcacat ttgcttctgc ttgctgcagt ccggattcgc gaacgcgtct
     5641 cgagagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     5701 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     5761 aaggaggaac tatatccgac tagtagcccg cctaatgagc gggctttttt ttaattcccc
     5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5881 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5941 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctcgt
     6001 gaaagtaaaa gacgcagagg accaattggg ggcacgagtg ggatacatag aactggactt
     6061 gaatagcggt aaaatccttg agagttttcg ccctgaagag cgttttccaa tgatgagcac
     6121 tttcaaagtt ctgctatgtg gagcagtatt atcccgtgta gatgcggggc aagagcaact
     6181 cggacgacga atacactatt cgcagaatga cttggttgaa tactccccag tgacagaaaa
     6241 gcaccttacg gacggaatga cggtaagaga attatgtagt gccgccataa cgatgagtga
     6301 taacactgcg gcgaacttac ttctgacaac catcggtgga ccgaaggaat taaccgcttt
     6361 tttgcacaat atgggagacc atgtaactcg ccttgaccgt tgggaaccag aactgaatga
     6421 agccatacca aacgacgagc gagacaccac aatgcctgcg gcaatggcaa caacattacg
     6481 caaactatta actggcgaac tacttactct ggcttcacgg caacaattaa tagactggct
     6541 tgaagcggat aaagttgcag gaccactact gcgttcggca cttcctgctg gctggtttat
     6601 tgctgataaa tctggggcag gagagcgtgg ttcacggggt atcattgccg cacttggacc
     6661 agatggtaag ccttcccgta tcgtagttat ctacacgacg ggtagtcagg caactatgga
     6721 cgaacgaaat agacagattg ctgaaatagg ggcttcactg attaagcatt ggtaaaccga
     6781 tacaattaaa ggctcctttt ggagcctttt tttttggacg gaccggtaga aaagatcaaa
     6841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6961 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7441 gccagcaacg cggccttttt acggttcctg gccttttgct ggcct
//
