LOCUS       sequence-445894-        7571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN and GvpG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNG
FEATURES             Location/Qualifiers
     source          1..7571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2319..2336
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5829..5847)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5843..5890
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5957..6048
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6913..6952
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6983..7571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7472..7491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gaggagcttg aaaacatgag tgaaacaaac gaaacaggta tttatatttt tagcgccatt
     1801 caaacggata aagacgaaga atttggcgcc gtggaagtag aaggaacaaa agctgaaaca
     1861 tttttgattc gctacaaaga cgcggctatg gtagcagctg aagtaccgat gaaaatttat
     1921 catcctaatc gccaaaattt attaatgcat caaaacgcag tagcagcgat tatggacaag
     1981 aacgatacgg ttattccaat cagctttggg aatgtattca aatcaaaaga agacgtaaaa
     2041 gttcttttgg aaaaccttta tccgcagttt gaaaagctgt ttccagcgat caaaggaaaa
     2101 attgaagtcg gtttaaaagt aattgggaaa aaagaatggc ttgagaaaaa agtaaacgaa
     2161 aatcctgaac ttgagaaagt atcagcatcc gtaaaaggaa aatcagaagc agccggttat
     2221 tatgagcgta ttcaacttgg aggaatggct caaaagatgt ttacttccct gcaaaaagaa
     2281 gtcaagacag atgtgttttc tccgcttgaa gaagcagcgg aagcagcaaa agcaaatgag
     2341 ccaacgggcg aaacgatgct tttaaacgcg tctttcttaa ttaaccgaga agatgaagcg
     2401 aagtttgatg aaaaagtaaa tgaagcgcat gaaaactgga aagacaaagc cgattttcat
     2461 tacagcggtc cttggcctgc ttataatttt gtgaacattc gcctaaaagt agaagagaaa
     2521 taacgaaatg aggaatccta gatgggagaa ttactgtatt tatacggttt aattccaaca
     2581 aaagaagcag cagccataga gccgtttcca tcttataagg ggtttgacgg agaacattca
     2641 ctgtacccaa ttgcgtttga tcaggtgacg gctgtagttt ctaagctgga tgctgacacc
     2701 tattcagaaa aagtgattca agaaaaaatg gagcaggata tgagctggct gcaggaaaaa
     2761 gcatttcatc atcacgaaac ggtagccgct ttgtacgaag aatttacgat cattccatta
     2821 aaattttgca ccatttataa aggtgaagaa agtctgcagg cagctattga gattaacaaa
     2881 gaaaagatag agaattcact gacgctgctt caaggaaatg aagagtggaa tgtgaaaatt
     2941 tactgtgatg atacagagct taaaaaagga atcagcgaaa cgaatgaaag cgtgaaagcg
     3001 aaaaaacaag aaattagtca cttatcacca ggaagacagt tttttgaaaa gaaaaaaata
     3061 gatcagctga ttgaaaaaga attagagctt cacaaaaaca aagtgtgtga agagatacat
     3121 gacaagctaa aagaattatc gctttatgac tctgttaaaa agaattggag caaagacgta
     3181 actggcgcag ctgaacagat ggcgtggaac agcgtgtttc ttctcccgtc tctgcagatt
     3241 actaagttcg taaacgaaat agaagagctt cagcaaaggc ttgaaaataa aggctggaag
     3301 tttgaagtga cgggaccatg gccgccctat catttctcga gctttgcgta aagtgaggaa
     3361 ttaacattat gtctcttaaa caatccatgg agaataaaga tattgctctt attgatattt
     3421 tagatgtcat tttagataaa ggagtcgcca ttaaaggaga cttaatcatt tccatagctg
     3481 gcgtcgattt agtgtatttg gatttgcggg tgcttatttc ttcggttgaa acgcttgtgc
     3541 aagcaaaaga aggaaatcac aaaccaatca cttctgaaca atttgataaa caaaaggagg
     3601 aattaatgga tgcaaccggt cagccaagca aatggacgaa tccacttgga tcctgatcaa
     3661 gctgaacaag gcttagcgca gcttgtgatg acagttattg agctattgag gcaaatcgtt
     3721 gaacgtcatg ccatgaggcg ggtggagggt ggaacgttga cggacgaaca aattgaaaac
     3781 ttaggaattg cactaatgaa cttagaagaa aaaatggacg agttgaaaga ggtgttcggt
     3841 ctggatgcag aagatttaaa tattgatctt ggaccgctag gcagcctgct ttaagcggtc
     3901 agtaggagga acagtatggc agtcgaacat aatatgcagt caagtacgat tgtagatgtg
     3961 ctcgaaaaga ttttggataa aggagtcgtt atagcggggg acatcaccgt aggaattgca
     4021 gatgtcgagc tattaacgat aaagatccgc ttgattgtgg cttcggttga taaggcaaaa
     4081 gaaatcggca tggactggtg ggaaaatgat ccgtatctca gttcaaaagg agccaataac
     4141 aaagcgctcg aagaagaaaa taaaatgctg catgagcggt taaaaacgct tgaagaaaaa
     4201 atagaaacga aacgttaaaa actgtacgct acttaaaaaa tggagggatt tacaatggca
     4261 actgaaacaa aattagataa cacacaggca gaaaacaagg aaaataaaaa tgcggaaaac
     4321 ggttcaaaag aaaagaacgg ttcaaaagca agcaaaacaa caagcagcgg gccaatcaaa
     4381 cgagcggtag caggaggcat catcggtgca acgattggat atgtatcgac tcctgaaaat
     4441 cgaaaaagtc tccttgaccg cattgataca gacgaattaa aaagcaaagc atctgattta
     4501 ggaacaaagg taaaagaaaa atcaaaaagc agcgtggcca gcctgaaaac atctgcggga
     4561 agcttgttta aaaaagataa agataaatca aaagatgatg aagaaaacgt aaattcttct
     4621 agtagcgaaa cagaagacga taacgttcaa gagtacgacg agttaaaaga agaaaatcaa
     4681 actcttcaag atcgcttatc acagcttgaa gaaaaaatga acatgcttgt tgagcttagc
     4741 ctcaataaaa atcaagacga agaagcggaa gatacagatt ccgacgaaga agagaacgat
     4801 gagaacgatg aaaacgatga aaacgagcag gacgatgaaa acgaagaaga aacatctaag
     4861 ccacgtaaaa aggataaaaa agaagctgag gaagaagaaa gtgaaagtga cgaagacagc
     4921 gaggaagaag aggaagattc tcgctcaaac aaaaaaaata aaaaagtaaa aacagaagaa
     4981 gaagacgaag atgaaagcga agaagaaaaa aaggaagcga aaccaaaaaa gtcaacagct
     5041 aaaaaatcaa aaaatacaaa agcaaagaaa aacacggacg aagaagatga tgaagcaaca
     5101 tctctttcta gtgaagacga tacaacagcc taagacgtaa aggaggaaag aaagacatga
     5161 gtacaggccc ttctttttca actaaagaca atacgcttga atactttgtg aaagcttcta
     5221 ataaacacgg cttttcactt gatatttcat taaatgtaaa cggcgctgtg atttccggta
     5281 ccatgatttc agcaaaagaa tactttgatt acttaagcga aacgtttgaa gaaggcagtg
     5341 aagtggctca ggcgctaagc gaacaattct ctttagcaag cgaagcgagc gaatcaaacg
     5401 gagaagcaga agcccatttt attcatttga aaaatacaaa gatttactgt ggagacagta
     5461 aatctactcc ttctaaaggc aaaatctttt ggagagggaa aatagcagaa gtagacgggt
     5521 ttttcttagg aaagatttct gatgcaaaat caacgagtaa aaagagttca taaaaaacgg
     5581 cggggtgatt gccccgccgt tttttagtga tgtgatgaga tgtgcagctt cttttttcga
     5641 tacatgagcg gagtcatctt catttctttt cgaaactgat taataaaata gcttgtacta
     5701 ttaaacccta cttgataggc gacttccgtc acatttgctt ctgcttgctg cagtccggat
     5761 tcgcgaacgc gtctcgagag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     5821 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     5881 gggttttttg ctgaaaggag gaactatatc cgactagtag cccgcctaat gagcgggctt
     5941 ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6001 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6061 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6121 ccagaaacgc tcgtgaaagt aaaagacgca gaggaccaat tgggggcacg agtgggatac
     6181 atagaactgg acttgaatag cggtaaaatc cttgagagtt ttcgccctga agagcgtttt
     6241 ccaatgatga gcactttcaa agttctgcta tgtggagcag tattatcccg tgtagatgcg
     6301 gggcaagagc aactcggacg acgaatacac tattcgcaga atgacttggt tgaatactcc
     6361 ccagtgacag aaaagcacct tacggacgga atgacggtaa gagaattatg tagtgccgcc
     6421 ataacgatga gtgataacac tgcggcgaac ttacttctga caaccatcgg tggaccgaag
     6481 gaattaaccg cttttttgca caatatggga gaccatgtaa ctcgccttga ccgttgggaa
     6541 ccagaactga atgaagccat accaaacgac gagcgagaca ccacaatgcc tgcggcaatg
     6601 gcaacaacat tacgcaaact attaactggc gaactactta ctctggcttc acggcaacaa
     6661 ttaatagact ggcttgaagc ggataaagtt gcaggaccac tactgcgttc ggcacttcct
     6721 gctggctggt ttattgctga taaatctggg gcaggagagc gtggttcacg gggtatcatt
     6781 gccgcacttg gaccagatgg taagccttcc cgtatcgtag ttatctacac gacgggtagt
     6841 caggcaacta tggacgaacg aaatagacag attgctgaaa taggggcttc actgattaag
     6901 cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg gacggaccgg
     6961 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7021 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7081 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7141 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7201 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7261 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7321 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7381 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7441 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7501 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7561 gcctatggaa a
//
