LOCUS       sequence-445897-        7662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB and GvpF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBF
FEATURES             Location/Qualifiers
     source          1..7662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1308..1326)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1322..1369
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1436..1527
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2392..2431
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      2462..3050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2951..2970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3204..3221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3236..3375
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3301..3320)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3461..3483
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3477..3668)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3905..3924
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3905..3923
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcaaaaagca gcgtggccag cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa
       61 gataaatcaa aagatgatga agaaaacgta aattcttcta gtagcgaaac agaagacgat
      121 aacgttcaag agtacgacga gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca
      181 cagcttgaag aaaaaatgaa catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa
      241 gaagcggaag atacagattc cgacgaagaa gagaacgatg agaacgatga aaacgatgaa
      301 aacgagcagg acgatgaaaa cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa
      361 gaagctgagg aagaagaaag tgaaagtgac gaagacagcg aggaagaaga ggaagattct
      421 cgctcaaaca aaaaaaataa aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa
      481 gaagaaaaaa aggaagcgaa accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa
      541 gcaaagaaaa acacggacga agaagatgat gaagcaacat ctctttctag tgaagacgat
      601 acaacagcct aagacgtaaa ggaggaaaga aagacatgag tacaggccct tctttttcaa
      661 ctaaagacaa tacgcttgaa tactttgtga aagcttctaa taaacacggc ttttcacttg
      721 atatttcatt aaatgtaaac ggcgctgtga tttccggtac catgatttca gcaaaagaat
      781 actttgatta cttaagcgaa acgtttgaag aaggcagtga agtggctcag gcgctaagcg
      841 aacaattctc tttagcaagc gaagcgagcg aatcaaacgg agaagcagaa gcccatttta
      901 ttcatttgaa aaatacaaag atttactgtg gagacagtaa atctactcct tctaaaggca
      961 aaatcttttg gagagggaaa atagcagaag tagacgggtt tttcttagga aagatttctg
     1021 atgcaaaatc aacgagtaaa aagagttcat aaaaaacggc ggggtgattg ccccgccgtt
     1081 ttttagtgat gtgatgagat gtgcagcttc ttttttcgat acatgagcgg agtcatcttc
     1141 atttcttttc gaaactgatt aataaaatag cttgtactat taaaccctac ttgataggcg
     1201 acttccgtca catttgcttc tgcttgctgc agtccggatt cgcgaacgcg tctcgagaga
     1261 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     1321 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     1381 aactatatcc gactagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
     1441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1501 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1561 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta
     1621 aaagacgcag aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc
     1681 ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa
     1741 gttctgctat gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga
     1801 cgaatacact attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt
     1861 acggacggaa tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact
     1921 gcggcgaact tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac
     1981 aatatgggag accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata
     2041 ccaaacgacg agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta
     2101 ttaactggcg aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg
     2161 gataaagttg caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat
     2221 aaatctgggg caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt
     2281 aagccttccc gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga
     2341 aatagacaga ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt
     2401 aaaggctcct tttggagcct ttttttttgg acggaccggt agaaaagatc aaaggatctt
     2461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2581 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3061 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     3241 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     3301 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     3361 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3421 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3481 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     3541 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     3601 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     3661 ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga
     3721 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     3781 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     3841 gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat cgatcccgcg
     3901 aaattaatac gactcactat agggagacca caacggtttc cctctagaga tagcttagcg
     3961 atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac gataaagaga
     4021 agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca acggatatta
     4081 aaagctctac taagattaaa agttctaatg atattaaatc atctacagat ttaaaaacaa
     4141 tgggatcatt agatctaact attggaggct actaaaaatg gaaattaaaa aaattatgca
     4201 agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta cctcggtgga
     4261 agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac gagaatatat
     4321 gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa ataaagaaaa
     4381 agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg gcattgaagc
     4441 ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac aaaaggaaaa
     4501 aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca agagcgctga
     4561 gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga ggcaaaacct
     4621 ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg cacgggaatc
     4681 acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc aaaaaagtaa
     4741 tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa aactggcagg
     4801 atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac gaatttactc
     4861 gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc gtgctgccgc
     4921 tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc cgcgtcatct
     4981 tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg cttctcgaca
     5041 ggttaattac catgtttatt gattataaag acatcgacag agagacagcg attttaacgg
     5101 agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta gcaaacgtgc
     5161 gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg cttatgatcg
     5221 ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat tttcaaacgt
     5281 tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa aatgcaaaaa
     5341 gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa gaaaagtaag
     5401 tagaagagaa ataacgtgct tcacaaatta gtaaccgcac ccattaacct tgtagtgaaa
     5461 atcggcgaaa aagtacagga agaagctgat aaacagctat atgaccttcc gacgattcag
     5521 caaaagctca ttcagcttca aatgatgttt gagcttggtg aaattccaga agaagcgttt
     5581 caagaaaaag aagatgaatt gttaatgagg tacgaaattg cgaaacgcag agaaattgaa
     5641 caatgggaag agctaacaca aaaaagaaat gaggaatcct agatgggaga attactgtat
     5701 ttatacggtt taattccaac aaaagaagca gcagccatag agccgtttcc atcttataag
     5761 gggtttgacg gagaacattc actgtaccca attgcgtttg atcaggtgac ggctgtagtt
     5821 tctaagctgg atgctgacac ctattcagaa aaagtgattc aagaaaaaat ggagcaggat
     5881 atgagctggc tgcaggaaaa agcatttcat catcacgaaa cggtagccgc tttgtacgaa
     5941 gaatttacga tcattccatt aaaattttgc accatttata aaggtgaaga aagtctgcag
     6001 gcagctattg agattaacaa agaaaagata gagaattcac tgacgctgct tcaaggaaat
     6061 gaagagtgga atgtgaaaat ttactgtgat gatacagagc ttaaaaaagg aatcagcgaa
     6121 acgaatgaaa gcgtgaaagc gaaaaaacaa gaaattagtc acttatcacc aggaagacag
     6181 ttttttgaaa agaaaaaaat agatcagctg attgaaaaag aattagagct tcacaaaaac
     6241 aaagtgtgtg aagagataca tgacaagcta aaagaattat cgctttatga ctctgttaaa
     6301 aagaattgga gcaaagacgt aactggcgca gctgaacaga tggcgtggaa cagcgtgttt
     6361 cttctcccgt ctctgcagat tactaagttc gtaaacgaaa tagaagagct tcagcaaagg
     6421 cttgaaaata aaggctggaa gtttgaagtg acgggaccat ggccgcccta tcatttctcg
     6481 agctttgcgt aaagtgagga attaacatta tgtctcttaa acaatccatg gagaataaag
     6541 atattgctct tattgatatt ttagatgtca ttttagataa aggagtcgcc attaaaggag
     6601 acttaatcat ttccatagct ggcgtcgatt tagtgtattt ggatttgcgg gtgcttattt
     6661 cttcggttga aacgcttgtg caagcaaaag aaggaaatca caaaccaatc acttctgaac
     6721 aatttgataa acaaaaggag gaattaatgg atgcaaccgg tcagccaagc aaatggacga
     6781 atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat gacagttatt
     6841 gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg tggaacgttg
     6901 acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga aaaaatggac
     6961 gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct tggaccgcta
     7021 ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca taatatgcag
     7081 tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt tatagcgggg
     7141 gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg cttgattgtg
     7201 gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga tccgtatctc
     7261 agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct gcatgagcgg
     7321 ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc tacttaaaaa
     7381 atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc agaaaacaag
     7441 gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc aagcaaaaca
     7501 acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc aacgattgga
     7561 tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac agacgaatta
     7621 aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aa
//
