LOCUS       sequence-445899-        8429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaB
FEATURES             Location/Qualifiers
     source          1..8429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             571..588
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(4331..4349)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4345..4392
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4459..4550
                     /gene="bla"
                     /label=AmpR promoter
     terminator      5415..5454
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      5485..6073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5974..5993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6227..6244
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6259..6398
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6324..6343)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6484..6506
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6500..6691)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     6928..6947
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6928..6946
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgaaaacatg agtgaaacaa acgaaacagg tatttatatt tttagcgcca ttcaaacgga
       61 taaagacgaa gaatttggcg ccgtggaagt agaaggaaca aaagctgaaa catttttgat
      121 tcgctacaaa gacgcggcta tggtagcagc tgaagtaccg atgaaaattt atcatcctaa
      181 tcgccaaaat ttattaatgc atcaaaacgc agtagcagcg attatggaca agaacgatac
      241 ggttattcca atcagctttg ggaatgtatt caaatcaaaa gaagacgtaa aagttctttt
      301 ggaaaacctt tatccgcagt ttgaaaagct gtttccagcg atcaaaggaa aaattgaagt
      361 cggtttaaaa gtaattggga aaaaagaatg gcttgagaaa aaagtaaacg aaaatcctga
      421 acttgagaaa gtatcagcat ccgtaaaagg aaaatcagaa gcagccggtt attatgagcg
      481 tattcaactt ggaggaatgg ctcaaaagat gtttacttcc ctgcaaaaag aagtcaagac
      541 agatgtgttt tctccgcttg aagaagcagc ggaagcagca aaagcaaatg agccaacggg
      601 cgaaacgatg cttttaaacg cgtctttctt aattaaccga gaagatgaag cgaagtttga
      661 tgaaaaagta aatgaagcgc atgaaaactg gaaagacaaa gccgattttc attacagcgg
      721 tccttggcct gcttataatt ttgtgaacat tcgcctaaaa gtagaagaga aataacgtgc
      781 ttcacaaatt agtaaccgca cccattaacc ttgtagtgaa aatcggcgaa aaagtacagg
      841 aagaagctga taaacagcta tatgaccttc cgacgattca gcaaaagctc attcagcttc
      901 aaatgatgtt tgagcttggt gaaattccag aagaagcgtt tcaagaaaaa gaagatgaat
      961 tgttaatgag gtacgaaatt gcgaaacgca gagaaattga acaatgggaa gagctaacac
     1021 aaaaaagaaa tgaggaatcc tagatgggag aattactgta tttatacggt ttaattccaa
     1081 caaaagaagc agcagccata gagccgtttc catcttataa ggggtttgac ggagaacatt
     1141 cactgtaccc aattgcgttt gatcaggtga cggctgtagt ttctaagctg gatgctgaca
     1201 cctattcaga aaaagtgatt caagaaaaaa tggagcagga tatgagctgg ctgcaggaaa
     1261 aagcatttca tcatcacgaa acggtagccg ctttgtacga agaatttacg atcattccat
     1321 taaaattttg caccatttat aaaggtgaag aaagtctgca ggcagctatt gagattaaca
     1381 aagaaaagat agagaattca ctgacgctgc ttcaaggaaa tgaagagtgg aatgtgaaaa
     1441 tttactgtga tgatacagag cttaaaaaag gaatcagcga aacgaatgaa agcgtgaaag
     1501 cgaaaaaaca agaaattagt cacttatcac taggaagaca gttttttgaa aagaaaaaaa
     1561 tagatcagct gattgaaaaa gaattagagc ttcacaaaaa caaagtgtgt gaagagatac
     1621 atgacaagct aaaagaatta tcgctttatg actctgttaa aaagaattgg agcaaagacg
     1681 taactggcgc agctgaacag atggcgtgga acagcgtgtt tcttctcccg tctctgcaga
     1741 ttactaagtt cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat aaaggctgga
     1801 agtttgaagt gacgggacca tggccgccct atcatttctc gagctttgcg taaagtgagg
     1861 aattaacatt atgtctctta aacaatccat ggagaataaa gatattgctc ttattgatat
     1921 tttagatgtc attttagata aaggagtcgc cattaaagga gacttaatca tttccatagc
     1981 tggcgtcgat ttagtgtatt tggatttgcg ggtgcttatt tcttcggttg aaacgcttgt
     2041 gcaagcaaaa gaaggaaatc acaaaccaat cacttctgaa caatttgata aacaaaagga
     2101 ggaattaatg gatgcaaccg gtcagccaag caaatggacg aatccacttg gatcctgatc
     2161 aagctgaaca aggcttagcg cagcttgtga tgacagttat tgagctattg aggcaaatcg
     2221 ttgaacgtca tgccatgagg cgggtggagg gtggaacgtt gacggacgaa caaattgaaa
     2281 acttaggaat tgcactaatg aacttagaag aaaaaatgga cgagttgaaa gaggtgttcg
     2341 gtctggatgc agaagattta aatattgatc ttggaccgct aggcagcctg ctttaagcgg
     2401 tcagtaggag gaacagtatg gcagtcgaac ataatatgca gtcaagtacg attgtagatg
     2461 tgctcgaaaa gattttggat aaaggagtcg ttatagcggg ggacatcacc gtaggaattg
     2521 cagatgtcga gctattaacg ataaagatcc gcttgattgt ggcttcggtt gataaggcaa
     2581 aagaaatcgg catggactgg tgggaaaatg atccgtatct cagttcaaaa ggagccaata
     2641 acaaagcgct cgaagaagaa aataaaatgc tgcatgagcg gttaaaaacg cttgaagaaa
     2701 aaatagaaac gaaacgttaa aaactgtacg ctacttaaaa aatggaggga tttacaatgg
     2761 caactgaaac aaaattagat aacacacagg cagaaaacaa ggaaaataaa aatgcggaaa
     2821 acggttcaaa agaaaagaac ggttcaaaag caagcaaaac aacaagcagc gggccaatca
     2881 aacgagcggt agcaggaggc atcatcggtg caacgattgg atatgtatcg actcctgaaa
     2941 atcgaaaaag tctccttgac cgcattgata cagacgaatt aaaaagcaaa gcatctgatt
     3001 taggaacaaa ggtaaaagaa aaatcaaaaa gcagcgtggc cagcctgaaa acatctgcgg
     3061 gaagcttgtt taaaaaagat aaagataaat caaaagatga tgaagaaaac gtaaattctt
     3121 ctagtagcga aacagaagac gataacgttc aagagtacga cgagttaaaa gaagaaaatc
     3181 aaactcttca agatcgctta tcacagcttg aagaaaaaat gaacatgctt gttgagctta
     3241 gcctcaataa aaatcaagac gaagaagcgg aagatacaga ttccgacgaa gaagagaacg
     3301 atgagaacga tgaaaacgat gaaaacgagc aggacgatga aaacgaagaa gaaacatcta
     3361 agccacgtaa aaaggataaa aaagaagctg aggaagaaga aagtgaaagt gacgaagaca
     3421 gcgaggaaga agaggaagat tctcgctcaa acaaaaaaaa taaaaaagta aaaacagaag
     3481 aagaagacga agatgaaagc gaagaagaaa aaaaggaagc gaaaccaaaa aagtcaacag
     3541 ctaaaaaatc aaaaaataca aaagcaaaga aaaacacgga cgaagaagat gatgaagcaa
     3601 catctctttc tagtgaagac gatacaacag cctaagacgt aaaggaggaa agaaagacat
     3661 gagtacaggc ccttcttttt caactaaaga caatacgctt gaatactttg tgaaagcttc
     3721 taataaacac ggcttttcac ttgatatttc attaaatgta aacggcgctg tgatttccgg
     3781 taccatgatt tcagcaaaag aatactttga ttacttaagc gaaacgtttg aagaaggcag
     3841 tgaagtggct caggcgctaa gcgaacaatt ctctttagca agcgaagcga gcgaatcaaa
     3901 cggagaagca gaagcccatt ttattcattt gaaaaataca aagatttact gtggagacag
     3961 taaatctact ccttctaaag gcaaaatctt ttggagaggg aaaatagcag aagtagacgg
     4021 gtttttctta ggaaagattt ctgatgcaaa atcaacgagt aaaaagagtt cataaaaaac
     4081 ggcggggtga ttgccccgcc gttttttagt gatgtgatga gatgtgcagc ttcttttttc
     4141 gatacatgag cggagtcatc ttcatttctt ttcgaaactg attaataaaa tagcttgtac
     4201 tattaaaccc tacttgatag gcgacttccg tcacatttgc ttctgcttgc tgcagtccgg
     4261 attcgcgaac gcgtctcgag agatccggct gctaacaaag cccgaaagga agctgagttg
     4321 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     4381 aggggttttt tgctgaaagg aggaactata tccgactagt agcccgccta atgagcgggc
     4441 ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4501 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4561 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4621 acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca cgagtgggat
     4681 acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct gaagagcgtt
     4741 ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc cgtgtagatg
     4801 cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg gttgaatact
     4861 ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta tgtagtgccg
     4921 ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc ggtggaccga
     4981 aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt gaccgttggg
     5041 aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg cctgcggcaa
     5101 tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct tcacggcaac
     5161 aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt tcggcacttc
     5221 ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca cggggtatca
     5281 ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac acgacgggta
     5341 gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct tcactgatta
     5401 agcattggta aaccgataca attaaaggct ccttttggag cctttttttt tggacggacc
     5461 ggtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5521 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5581 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5641 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5701 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5761 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5821 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5881 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5941 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6001 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6061 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6121 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6241 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6301 acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     6361 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     6421 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6481 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
     6541 tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
     6601 cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
     6661 tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt
     6721 tcatggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     6781 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     6841 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     6901 tggcaccatg catcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
     6961 ttccctctag agatagctta gcgatgtgaa agaagctgga gaaaactttc aagaaaaagt
     7021 ttcttcttca gacgataaag agaagtcaaa aaataagcgg aaaataaaag gcgtaaatca
     7081 gattaaaaaa gcaacggata ttaaaagctc tactaagatt aaaagttcta atgatattaa
     7141 atcatctaca gatttaaaaa caatgggatc attagatcta actattggag gctactaaaa
     7201 atggaaatta aaaaaattat gcaagccgtg aacgactttt tcggtgaaca cgtagctcct
     7261 cctcataaaa ttacctcggt ggaagctact gaagatgaag gttggagagt tattgttgaa
     7321 gtcattgaag aacgagaata tatgaaaaaa tacgccaaag atgaaatgct cggaacgtac
     7381 gagtgctttg taaataaaga aaaagaagtc atttcattca aacgactcga cgtcagatat
     7441 agaagcgcca ttggcattga agcataaacg ataagatggc aggaggaacg taaaaatgac
     7501 cgtcttaaca gacaaaagga aaaaaggcag tggagctttt atacaagatg acgagacaaa
     7561 agaggttctt tcaagagcgc tgagctattt aaaatccggc tattccattc attttacagg
     7621 tcctgccggc ggaggcaaaa cctctttagc gcgagcgctt gctaaaaaga gaaagcgtcc
     7681 tgtaatgctg atgcacggga atcacgagct caacaacaaa gatttaattg gcgattttac
     7741 gggatacacg agcaaaaaag taatcgacca gtacgttcgt tctgtctata aaaaagatga
     7801 acaggtgagt gaaaactggc aggatggccg attgcttgaa gctgtaaaaa atggctatac
     7861 gctgatttac gacgaattta ctcgttctaa gcctgcgacg aataatatct ttctatcgat
     7921 attagaagaa ggcgtgctgc cgctgtatgg agtaaaaatg accgatcctt ttgtgcgcgt
     7981 gcatcccgat ttccgcgtca tcttcacaag caatccagct gagtatgccg gcgtatatga
     8041 tacgcaagat gcgcttctcg acaggttaat taccatgttt attgattata aagacatcga
     8101 cagagagaca gcgattttaa cggagaaaac ggacgtagaa gaagatgaag cgcgcacaat
     8161 tgtaacgctc gtagcaaacg tgcgaaaccg ctctggagac gaaaacagca gcggacttag
     8221 cctgcgggct tcgcttatga tcgctaccct tgccacgcag caagacattc ctatcgatgg
     8281 aagtgacgaa gattttcaaa cgttatgtat cgatattttg catcatccgc ttaccaaatg
     8341 tttggatgaa gaaaatgcaa aaagcaaagc cgaaaaaatc attttagaag aatgtaagaa
     8401 tatagacact gaagaaaagt aaaggagct
//
