LOCUS       sequence-44590-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: si:ch211-221n23.1_R (OZ604)
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..49
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(538..557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaaatccaag ctttggcggc cgctgtacta caaagacgat gatgacaaat ctagacccgg
       61 ggagcgcccc ttccagtgtc gcatttgcat gcggaacttt tcgaagaaga ccaaccttac
      121 caggcatacc cgtactcata ccggtgaaaa accgtttcag tgtcggatct gtatgcgaaa
      181 tttctcccag aacgagacct tgaagaggca tctacgtacg cacaccggcg agaagccatt
      241 ccaatgccga atatgcatgc gcaacttcag tgtcggcgcc agcctgaaac gccacctaaa
      301 aacccacctg aggggatcct ctgcacagta gtaactagag actagaaaaa ggccgacaag
      361 tcccgctccg ctgaagatcc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      421 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac ccc
//
