LOCUS       sequence-445902-        7821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaN
FEATURES             Location/Qualifiers
     source          1..7821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..83
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    98..237
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(163..182)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     323..345
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(339..530)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     767..786
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        767..785
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2231..2248
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5991..6009)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6005..6052
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6119..6210
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7075..7114
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7145..7733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7634..7653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
       61 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
      121 cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc
      181 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      241 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      301 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      361 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
      421 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      481 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      541 gtgtaagggg gatttctgtt catggggtaa tgataccgat gaaacgagag aggatgctca
      601 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
      661 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
      721 ttaatacaga tgtaggtgtt ggcaccatgc atcgatcccg cgaaattaat acgactcact
      781 atagggagac cacaacggtt tccctctaga gatagcttag cgatgtgaaa gaagctggag
      841 aaaactttca agaaaaagtt tcttcttcag acgataaaga gaagtcaaaa aataagcgga
      901 aaataaaagg cgtaaatcag attaaaaaag caacggatat taaaagctct actaagatta
      961 aaagttctaa tgatattaaa tcatctacag atttaaaaac aatgggatca taagcgaaag
     1021 gagaaatgag atatgtctat tcaaaaaagt actaatagtt caagtttagc agaagtcatt
     1081 gaccgtattt tagataaagg aattgttatt gatgcttttg caagagtttc tgttgtagga
     1141 attgaaattt taacgattga agcgcgagtg gttattgcca gtgttgatac atggttacgc
     1201 tatgcagaag cagtagggct tcttcgtgac gacgtagaag aaaacggtct tcctgaacgt
     1261 tcaaattcaa gtgaagggca gccgcgtttt agtatttaat atgaagtggt atggaacctt
     1321 ccttttttga gggtggttcc agcttttaga tctaactatt ggaggctact aaaaatggaa
     1381 attaaaaaaa ttatgcaagc cgtgaacgac tttttcggtg aacacgtagc tcctcctcat
     1441 aaaattacct cggtggaagc tactgaagat gaaggttgga gagttattgt tgaagtcatt
     1501 gaagaacgag aatatatgaa aaaatacgcc aaagatgaaa tgctcggaac gtacgagtgc
     1561 tttgtaaata aagaaaaaga agtcatttca ttcaaacgac tcgacgtcag atatagaagc
     1621 gccattggca ttgaagcata aacgataaga tggaggagct tgaaaacatg agtgaaacaa
     1681 acgaaacagg tatttatatt tttagcgcca ttcaaacgga taaagacgaa gaatttggcg
     1741 ccgtggaagt agaaggaaca aaagctgaaa catttttgat tcgctacaaa gacgcggcta
     1801 tggtagcagc tgaagtaccg atgaaaattt atcatcctaa tcgccaaaat ttattaatgc
     1861 atcaaaacgc agtagcagcg attatggaca agaacgatac ggttattcca atcagctttg
     1921 ggaatgtatt caaatcaaaa gaagacgtaa aagttctttt ggaaaacctt tatccgcagt
     1981 ttgaaaagct gtttccagcg atcaaaggaa aaattgaagt cggtttaaaa gtaattggga
     2041 aaaaagaatg gcttgagaaa aaagtaaacg aaaatcctga acttgagaaa gtatcagcat
     2101 ccgtaaaagg aaaatcagaa gcagccggtt attatgagcg tattcaactt ggaggaatgg
     2161 ctcaaaagat gtttacttcc ctgcaaaaag aagtcaagac agatgtgttt tctccgcttg
     2221 aagaagcagc ggaagcagca aaagcaaatg agccaacggg cgaaacgatg cttttaaacg
     2281 cgtctttctt aattaaccga gaagatgaag cgaagtttga tgaaaaagta aatgaagcgc
     2341 atgaaaactg gaaagacaaa gccgattttc attacagcgg tccttggcct gcttataatt
     2401 ttgtgaacat tcgcctaaaa gtagaagaga aataacgtgc ttcacaaatt agtaaccgca
     2461 cccattaacc ttgtagtgaa aatcggcgaa aaagtacagg aagaagctga taaacagcta
     2521 tatgaccttc cgacgattca gcaaaagctc attcagcttc aaatgatgtt tgagcttggt
     2581 gaaattccag aagaagcgtt tcaagaaaaa gaagatgaat tgttaatgag gtacgaaatt
     2641 gcgaaacgca gagaaattga acaatgggaa gagctaacac aaaaaagaaa tgaggaatcc
     2701 tagatgggag aattactgta tttatacggt ttaattccaa caaaagaagc agcagccata
     2761 gagccgtttc catcttataa ggggtttgac ggagaacatt cactgtaccc aattgcgttt
     2821 gatcaggtga cggctgtagt ttctaagctg gatgctgaca cctattcaga aaaagtgatt
     2881 caagaaaaaa tggagcagga tatgagctgg ctgcaggaaa aagcatttca tcatcacgaa
     2941 acggtagccg ctttgtacga agaatttacg atcattccat taaaattttg caccatttat
     3001 aaaggtgaag aaagtctgca ggcagctatt gagattaaca aagaaaagat agagaattca
     3061 ctgacgctgc ttcaaggaaa tgaagagtgg aatgtgaaaa tttactgtga tgatacagag
     3121 cttaaaaaag gaatcagcga aacgaatgaa agcgtgaaag cgaaaaaaca agaaattagt
     3181 cacttatcac caggaagaca gttttttgaa aagaaaaaaa tagatcagct gattgaaaaa
     3241 gaattagagc ttcacaaaaa caaagtgtgt gaagagatac atgacaagct aaaagaatta
     3301 tcgctttatg actctgttaa aaagaattgg agcaaagacg taactggcgc agctgaacag
     3361 atggcgtgga acagcgtgtt tcttctcccg tctctgcaga ttactaagtt cgtaaacgaa
     3421 atagaagagc ttcagcaaag gcttgaaaat aaaggctgga agtttgaagt gacgggacca
     3481 tggccgccct atcatttctc gagctttgcg taaagtgagg aattaacatt atgtctctta
     3541 aacaatccat ggagaataaa gatattgctc ttattgatat tttagatgtc attttagata
     3601 aaggagtcgc cattaaagga gacttaatca tttccatagc tggcgtcgat ttagtgtatt
     3661 tggatttgcg ggtgcttatt tcttcggttg aaacgcttgt gcaagcaaaa gaaggaaatc
     3721 acaaaccaat cacttctgaa caatttgata aacaaaagga ggaattaatg gatgcaaccg
     3781 gtcagccaag caaatggacg aatccacttg gatcctgatc aagctgaaca aggcttagcg
     3841 cagcttgtga tgacagttat tgagctattg aggcaaatcg ttgaacgtca tgccatgagg
     3901 cgggtggagg gtggaacgtt gacggacgaa caaattgaaa acttaggaat tgcactaatg
     3961 aacttagaag aaaaaatgga cgagttgaaa gaggtgttcg gtctggatgc agaagattta
     4021 aatattgatc ttggaccgct aggcagcctg ctttaagcgg tcagtaggag gaacagtatg
     4081 gcagtcgaac ataatatgca gtcaagtacg attgtagatg tgctcgaaaa gattttggat
     4141 aaaggagtcg ttatagcggg ggacatcacc gtaggaattg cagatgtcga gctattaacg
     4201 ataaagatcc gcttgattgt ggcttcggtt gataaggcaa aagaaatcgg catggactgg
     4261 tgggaaaatg atccgtatct cagttcaaaa ggagccaata acaaagcgct cgaagaagaa
     4321 aataaaatgc tgcatgagcg gttaaaaacg cttgaagaaa aaatagaaac gaaacgttaa
     4381 aaactgtacg ctacttaaaa aatggaggga tttacaatgg caactgaaac aaaattagat
     4441 aacacacagg cagaaaacaa ggaaaataaa aatgcggaaa acggttcaaa agaaaagaac
     4501 ggttcaaaag caagcaaaac aacaagcagc gggccaatca aacgagcggt agcaggaggc
     4561 atcatcggtg caacgattgg atatgtatcg actcctgaaa atcgaaaaag tctccttgac
     4621 cgcattgata cagacgaatt aaaaagcaaa gcatctgatt taggaacaaa ggtaaaagaa
     4681 aaatcaaaaa gcagcgtggc cagcctgaaa acatctgcgg gaagcttgtt taaaaaagat
     4741 aaagataaat caaaagatga tgaagaaaac gtaaattctt ctagtagcga aacagaagac
     4801 gataacgttc aagagtacga cgagttaaaa gaagaaaatc aaactcttca agatcgctta
     4861 tcacagcttg aagaaaaaat gaacatgctt gttgagctta gcctcaataa aaatcaagac
     4921 gaagaagcgg aagatacaga ttccgacgaa gaagagaacg atgagaacga tgaaaacgat
     4981 gaaaacgagc aggacgatga aaacgaagaa gaaacatcta agccacgtaa aaaggataaa
     5041 aaagaagctg aggaagaaga aagtgaaagt gacgaagaca gcgaggaaga agaggaagat
     5101 tctcgctcaa acaaaaaaaa taaaaaagta aaaacagaag aagaagacga agatgaaagc
     5161 gaagaagaaa aaaaggaagc gaaaccaaaa aagtcaacag ctaaaaaatc aaaaaataca
     5221 aaagcaaaga aaaacacgga cgaagaagat gatgaagcaa catctctttc tagtgaagac
     5281 gatacaacag cctaagacgt aaaggaggaa agaaagacat gagtacaggc ccttcttttt
     5341 caactaaaga caatacgctt gaatactttg tgaaagcttc taataaacac ggcttttcac
     5401 ttgatatttc attaaatgta aacggcgctg tgatttccgg taccatgatt tcagcaaaag
     5461 aatactttga ttacttaagc gaaacgtttg aagaaggcag tgaagtggct caggcgctaa
     5521 gcgaacaatt ctctttagca agcgaagcga gcgaatcaaa cggagaagca gaagcccatt
     5581 ttattcattt gaaaaataca aagatttact gtggagacag taaatctact ccttctaaag
     5641 gcaaaatctt ttggagaggg aaaatagcag aagtagacgg gtttttctta ggaaagattt
     5701 ctgatgcaaa atcaacgagt aaaaagagtt cataaaaaac ggcggggtga ttgccccgcc
     5761 gttttttagt gatgtgatga gatgtgcagc ttcttttttc gatacatgag cggagtcatc
     5821 ttcatttctt ttcgaaactg attaataaaa tagcttgtac tattaaaccc tacttgatag
     5881 gcgacttccg tcacatttgc ttctgcttgc tgcagtccgg attcgcgaac gcgtctcgag
     5941 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     6001 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     6061 aggaactata tccgactagt agcccgccta atgagcgggc ttttttttaa ttcccctatt
     6121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctcgtgaaa
     6301 gtaaaagacg cagaggacca attgggggca cgagtgggat acatagaact ggacttgaat
     6361 agcggtaaaa tccttgagag ttttcgccct gaagagcgtt ttccaatgat gagcactttc
     6421 aaagttctgc tatgtggagc agtattatcc cgtgtagatg cggggcaaga gcaactcgga
     6481 cgacgaatac actattcgca gaatgacttg gttgaatact ccccagtgac agaaaagcac
     6541 cttacggacg gaatgacggt aagagaatta tgtagtgccg ccataacgat gagtgataac
     6601 actgcggcga acttacttct gacaaccatc ggtggaccga aggaattaac cgcttttttg
     6661 cacaatatgg gagaccatgt aactcgcctt gaccgttggg aaccagaact gaatgaagcc
     6721 ataccaaacg acgagcgaga caccacaatg cctgcggcaa tggcaacaac attacgcaaa
     6781 ctattaactg gcgaactact tactctggct tcacggcaac aattaataga ctggcttgaa
     6841 gcggataaag ttgcaggacc actactgcgt tcggcacttc ctgctggctg gtttattgct
     6901 gataaatctg gggcaggaga gcgtggttca cggggtatca ttgccgcact tggaccagat
     6961 ggtaagcctt cccgtatcgt agttatctac acgacgggta gtcaggcaac tatggacgaa
     7021 cgaaatagac agattgctga aataggggct tcactgatta agcattggta aaccgataca
     7081 attaaaggct ccttttggag cctttttttt tggacggacc ggtagaaaag atcaaaggat
     7141 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7261 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7501 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7561 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7621 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7681 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7741 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7801 ctgcgttatc ccctgattct g
//
