LOCUS       sequence-445904-        7602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNS
FEATURES             Location/Qualifiers
     source          1..7602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2321..2338
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5860..5878)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5874..5921
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5988..6079
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6944..6983
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7014..7602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7503..7522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcagggagct tgaaaacatg agtgaaacaa acgaaacagg tatttatatt tttagcgcca
     1801 ttcaaacgga taaagacgaa gaatttggcg ccgtggaagt agaaggaaca aaagctgaaa
     1861 catttttgat tcgctacaaa gacgcggcta tggtagcagc tgaagtaccg atgaaaattt
     1921 atcatcctaa tcgccaaaat ttattaatgc atcaaaacgc agtagcagcg attatggaca
     1981 agaacgatac ggttattcca atcagctttg ggaatgtatt caaatcaaaa gaagacgtaa
     2041 aagttctttt ggaaaacctt tatccgcagt ttgaaaagct gtttccagcg atcaaaggaa
     2101 aaattgaagt cggtttaaaa gtaattggga aaaaagaatg gcttgagaaa aaagtaaacg
     2161 aaaatcctga acttgagaaa gtatcagcat ccgtaaaagg aaaatcagaa gcagccggtt
     2221 attatgagcg tattcaactt ggaggaatgg ctcaaaagat gtttacttcc ctgcaaaaag
     2281 aagtcaagac agatgtgttt tctccgcttg aagaagcagc ggaagcagca aaagcaaatg
     2341 agccaacggg cgaaacgatg cttttaaacg cgtctttctt aattaaccga gaagatgaag
     2401 cgaagtttga tgaaaaagta aatgaagcgc atgaaaactg gaaagacaaa gccgattttc
     2461 attacagcgg tccttggcct gcttataatt ttgtgaacat tcgcctaaaa gtagaagaga
     2521 aataacgtgc ttcacaaatt agtaaccgca cccattaacc ttgtagtgaa aatcggcgaa
     2581 aaagtacagg aagaagctga taaacagcta tatgaccttc cgacgattca gcaaaagctc
     2641 attcagcttc aaatgatgtt tgagcttggt gaaattccag aagaagcgtt tcaagaaaaa
     2701 gaagatgaat tgttaatgag gtacgaaatt gcgaaacgca gagaaattga acaatgggaa
     2761 gagctaacac aaaaaagaaa tgaggaatcc tagatgggag aattactgta tttatacggt
     2821 ttaattccaa caaaagaagc agcagccata gagccgtttc catcttataa ggggtttgac
     2881 ggagaacatt cactgtaccc aattgcgttt gatcaggtga cggctgtagt ttctaagctg
     2941 gatgctgaca cctattcaga aaaagtgatt caagaaaaaa tggagcagga tatgagctgg
     3001 ctgcaggaaa aagcatttca tcatcacgaa acggtagccg ctttgtacga agaatttacg
     3061 atcattccat taaaattttg caccatttat aaaggtgaag aaagtctgca ggcagctatt
     3121 gagattaaca aagaaaagat agagaattca ctgacgctgc ttcaaggaaa tgaagagtgg
     3181 aatgtgaaaa tttactgtga tgatacagag cttaaaaaag gaatcagcga aacgaatgaa
     3241 agcgtgaaag cgaaaaaaca agaaattagt cacttatcac caggaagaca gttttttgaa
     3301 aagaaaaaaa tagatcagct gattgaaaaa gaattagagc ttcacaaaaa caaagtgtgt
     3361 gaagagatac atgacaagct aaaagaatta tcgctttatg actctgttaa aaagaattgg
     3421 agcaaagacg taactggcgc agctgaacag atggcgtgga acagcgtgtt tcttctcccg
     3481 tctctgcaga ttactaagtt cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat
     3541 aaaggctgga agtttgaagt gacgggacca tggccgccct atcatttctc gagctttgcg
     3601 taaaagcggt taagcgtgac agtccaggag gaattaatgg atgcaaccgg tcagccaagc
     3661 aaatggacga atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat
     3721 gacagttatt gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg
     3781 tggaacgttg acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga
     3841 aaaaatggac gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct
     3901 tggaccgcta ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca
     3961 taatatgcag tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt
     4021 tatagcgggg gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg
     4081 cttgattgtg gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga
     4141 tccgtatctc agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct
     4201 gcatgagcgg ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc
     4261 tacttaaaaa atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc
     4321 agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc
     4381 aagcaaaaca acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc
     4441 aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac
     4501 agacgaatta aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aatcaaaaag
     4561 cagcgtggcc agcctgaaaa catctgcggg aagcttgttt aaaaaagata aagataaatc
     4621 aaaagatgat gaagaaaacg taaattcttc tagtagcgaa acagaagacg ataacgttca
     4681 agagtacgac gagttaaaag aagaaaatca aactcttcaa gatcgcttat cacagcttga
     4741 agaaaaaatg aacatgcttg ttgagcttag cctcaataaa aatcaagacg aagaagcgga
     4801 agatacagat tccgacgaag aagagaacga tgagaacgat gaaaacgatg aaaacgagca
     4861 ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa aaggataaaa aagaagctga
     4921 ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa
     4981 caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa
     5041 aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa
     5101 aaacacggac gaagaagatg atgaagcaac atctctttct agtgaagacg atacaacagc
     5161 ctaagacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac
     5221 aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca
     5281 ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat
     5341 tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc
     5401 tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg
     5461 aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt
     5521 tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa
     5581 tcaacgagta aaaagagttc ataaaaaacg gcggggtgat tgccccgccg ttttttagtg
     5641 atgtgatgag atgtgcagct tcttttttcg atacatgagc ggagtcatct tcatttcttt
     5701 tcgaaactga ttaataaaat agcttgtact attaaaccct acttgatagg cgacttccgt
     5761 cacatttgct tctgcttgct gcagtccgga ttcgcgaacg cgtctcgaga gatccggctg
     5821 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     5881 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     5941 ccgactagta gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt
     6001 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6061 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6121 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctcgtgaaag taaaagacgc
     6181 agaggaccaa ttgggggcac gagtgggata catagaactg gacttgaata gcggtaaaat
     6241 ccttgagagt tttcgccctg aagagcgttt tccaatgatg agcactttca aagttctgct
     6301 atgtggagca gtattatccc gtgtagatgc ggggcaagag caactcggac gacgaataca
     6361 ctattcgcag aatgacttgg ttgaatactc cccagtgaca gaaaagcacc ttacggacgg
     6421 aatgacggta agagaattat gtagtgccgc cataacgatg agtgataaca ctgcggcgaa
     6481 cttacttctg acaaccatcg gtggaccgaa ggaattaacc gcttttttgc acaatatggg
     6541 agaccatgta actcgccttg accgttggga accagaactg aatgaagcca taccaaacga
     6601 cgagcgagac accacaatgc ctgcggcaat ggcaacaaca ttacgcaaac tattaactgg
     6661 cgaactactt actctggctt cacggcaaca attaatagac tggcttgaag cggataaagt
     6721 tgcaggacca ctactgcgtt cggcacttcc tgctggctgg tttattgctg ataaatctgg
     6781 ggcaggagag cgtggttcac ggggtatcat tgccgcactt ggaccagatg gtaagccttc
     6841 ccgtatcgta gttatctaca cgacgggtag tcaggcaact atggacgaac gaaatagaca
     6901 gattgctgaa ataggggctt cactgattaa gcattggtaa accgatacaa ttaaaggctc
     6961 cttttggagc cttttttttt ggacggaccg gtagaaaaga tcaaaggatc ttcttgagat
     7021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7141 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aa
//
