LOCUS       sequence-445905-        7638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN and GvpK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNK
FEATURES             Location/Qualifiers
     source          1..7638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2321..2338
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5896..5914)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5910..5957
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6024..6115
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6980..7019
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7050..7638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7539..7558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcagggagct tgaaaacatg agtgaaacaa acgaaacagg tatttatatt tttagcgcca
     1801 ttcaaacgga taaagacgaa gaatttggcg ccgtggaagt agaaggaaca aaagctgaaa
     1861 catttttgat tcgctacaaa gacgcggcta tggtagcagc tgaagtaccg atgaaaattt
     1921 atcatcctaa tcgccaaaat ttattaatgc atcaaaacgc agtagcagcg attatggaca
     1981 agaacgatac ggttattcca atcagctttg ggaatgtatt caaatcaaaa gaagacgtaa
     2041 aagttctttt ggaaaacctt tatccgcagt ttgaaaagct gtttccagcg atcaaaggaa
     2101 aaattgaagt cggtttaaaa gtaattggga aaaaagaatg gcttgagaaa aaagtaaacg
     2161 aaaatcctga acttgagaaa gtatcagcat ccgtaaaagg aaaatcagaa gcagccggtt
     2221 attatgagcg tattcaactt ggaggaatgg ctcaaaagat gtttacttcc ctgcaaaaag
     2281 aagtcaagac agatgtgttt tctccgcttg aagaagcagc ggaagcagca aaagcaaatg
     2341 agccaacggg cgaaacgatg cttttaaacg cgtctttctt aattaaccga gaagatgaag
     2401 cgaagtttga tgaaaaagta aatgaagcgc atgaaaactg gaaagacaaa gccgattttc
     2461 attacagcgg tccttggcct gcttataatt ttgtgaacat tcgcctaaaa gtagaagaga
     2521 aataacgtgc ttcacaaatt agtaaccgca cccattaacc ttgtagtgaa aatcggcgaa
     2581 aaagtacagg aagaagctga taaacagcta tatgaccttc cgacgattca gcaaaagctc
     2641 attcagcttc aaatgatgtt tgagcttggt gaaattccag aagaagcgtt tcaagaaaaa
     2701 gaagatgaat tgttaatgag gtacgaaatt gcgaaacgca gagaaattga acaatgggaa
     2761 gagctaacac aaaaaagaaa tgaggaatcc tagatgggag aattactgta tttatacggt
     2821 ttaattccaa caaaagaagc agcagccata gagccgtttc catcttataa ggggtttgac
     2881 ggagaacatt cactgtaccc aattgcgttt gatcaggtga cggctgtagt ttctaagctg
     2941 gatgctgaca cctattcaga aaaagtgatt caagaaaaaa tggagcagga tatgagctgg
     3001 ctgcaggaaa aagcatttca tcatcacgaa acggtagccg ctttgtacga agaatttacg
     3061 atcattccat taaaattttg caccatttat aaaggtgaag aaagtctgca ggcagctatt
     3121 gagattaaca aagaaaagat agagaattca ctgacgctgc ttcaaggaaa tgaagagtgg
     3181 aatgtgaaaa tttactgtga tgatacagag cttaaaaaag gaatcagcga aacgaatgaa
     3241 agcgtgaaag cgaaaaaaca agaaattagt cacttatcac caggaagaca gttttttgaa
     3301 aagaaaaaaa tagatcagct gattgaaaaa gaattagagc ttcacaaaaa caaagtgtgt
     3361 gaagagatac atgacaagct aaaagaatta tcgctttatg actctgttaa aaagaattgg
     3421 agcaaagacg taactggcgc agctgaacag atggcgtgga acagcgtgtt tcttctcccg
     3481 tctctgcaga ttactaagtt cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat
     3541 aaaggctgga agtttgaagt gacgggacca tggccgccct atcatttctc gagctttgcg
     3601 taaagtgagg aattaacatt atgtctctta aacaatccat ggagaataaa gatattgctc
     3661 ttattgatat tttagatgtc attttagata aaggagtcgc cattaaagga gacttaatca
     3721 tttccatagc tggcgtcgat ttagtgtatt tggatttgcg ggtgcttatt tcttcggttg
     3781 aaacgcttgt gcaagcaaaa gaaggaaatc acaaaccaat cacttctgaa caatttgata
     3841 aacaaaaaga agagctgatg gacgctacgg gccagcctag taagtggacg aaccctctag
     3901 gttcgtgacc cttgcgagca gtcgtatgct ttctcgaatt ccgagcggtt aagcgtgaca
     3961 ggcggtcagt aggaggaaca gtatggcagt cgaacataat atgcagtcaa gtacgattgt
     4021 agatgtgctc gaaaagattt tggataaagg agtcgttata gcgggggaca tcaccgtagg
     4081 aattgcagat gtcgagctat taacgataaa gatccgcttg attgtggctt cggttgataa
     4141 ggcaaaagaa atcggcatgg actggtggga aaatgatccg tatctcagtt caaaaggagc
     4201 caataacaaa gcgctcgaag aagaaaataa aatgctgcat gagcggttaa aaacgcttga
     4261 agaaaaaata gaaacgaaac gttaaaaact gtacgctact taaaaaatgg agggatttac
     4321 aatggcaact gaaacaaaat tagataacac acaggcagaa aacaaggaaa ataaaaatgc
     4381 ggaaaacggt tcaaaagaaa agaacggttc aaaagcaagc aaaacaacaa gcagcgggcc
     4441 aatcaaacga gcggtagcag gaggcatcat cggtgcaacg attggatatg tatcgactcc
     4501 tgaaaatcga aaaagtctcc ttgaccgcat tgatacagac gaattaaaaa gcaaagcatc
     4561 tgatttagga acaaaggtaa aagaaaaatc aaaaagcagc gtggccagcc tgaaaacatc
     4621 tgcgggaagc ttgtttaaaa aagataaaga taaatcaaaa gatgatgaag aaaacgtaaa
     4681 ttcttctagt agcgaaacag aagacgataa cgttcaagag tacgacgagt taaaagaaga
     4741 aaatcaaact cttcaagatc gcttatcaca gcttgaagaa aaaatgaaca tgcttgttga
     4801 gcttagcctc aataaaaatc aagacgaaga agcggaagat acagattccg acgaagaaga
     4861 gaacgatgag aacgatgaaa acgatgaaaa cgagcaggac gatgaaaacg aagaagaaac
     4921 atctaagcca cgtaaaaagg ataaaaaaga agctgaggaa gaagaaagtg aaagtgacga
     4981 agacagcgag gaagaagagg aagattctcg ctcaaacaaa aaaaataaaa aagtaaaaac
     5041 agaagaagaa gacgaagatg aaagcgaaga agaaaaaaag gaagcgaaac caaaaaagtc
     5101 aacagctaaa aaatcaaaaa atacaaaagc aaagaaaaac acggacgaag aagatgatga
     5161 agcaacatct ctttctagtg aagacgatac aacagcctaa gacgtaaagg aggaaagaaa
     5221 gacatgagta caggcccttc tttttcaact aaagacaata cgcttgaata ctttgtgaaa
     5281 gcttctaata aacacggctt ttcacttgat atttcattaa atgtaaacgg cgctgtgatt
     5341 tccggtacca tgatttcagc aaaagaatac tttgattact taagcgaaac gtttgaagaa
     5401 ggcagtgaag tggctcaggc gctaagcgaa caattctctt tagcaagcga agcgagcgaa
     5461 tcaaacggag aagcagaagc ccattttatt catttgaaaa atacaaagat ttactgtgga
     5521 gacagtaaat ctactccttc taaaggcaaa atcttttgga gagggaaaat agcagaagta
     5581 gacgggtttt tcttaggaaa gatttctgat gcaaaatcaa cgagtaaaaa gagttcataa
     5641 aaaacggcgg ggtgattgcc ccgccgtttt ttagtgatgt gatgagatgt gcagcttctt
     5701 ttttcgatac atgagcggag tcatcttcat ttcttttcga aactgattaa taaaatagct
     5761 tgtactatta aaccctactt gataggcgac ttccgtcaca tttgcttctg cttgctgcag
     5821 tccggattcg cgaacgcgtc tcgagagatc cggctgctaa caaagcccga aaggaagctg
     5881 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     5941 tcttgagggg ttttttgctg aaaggaggaa ctatatccga ctagtagccc gcctaatgag
     6001 cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6061 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6121 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6181 tgctcaccca gaaacgctcg tgaaagtaaa agacgcagag gaccaattgg gggcacgagt
     6241 gggatacata gaactggact tgaatagcgg taaaatcctt gagagttttc gccctgaaga
     6301 gcgttttcca atgatgagca ctttcaaagt tctgctatgt ggagcagtat tatcccgtgt
     6361 agatgcgggg caagagcaac tcggacgacg aatacactat tcgcagaatg acttggttga
     6421 atactcccca gtgacagaaa agcaccttac ggacggaatg acggtaagag aattatgtag
     6481 tgccgccata acgatgagtg ataacactgc ggcgaactta cttctgacaa ccatcggtgg
     6541 accgaaggaa ttaaccgctt ttttgcacaa tatgggagac catgtaactc gccttgaccg
     6601 ttgggaacca gaactgaatg aagccatacc aaacgacgag cgagacacca caatgcctgc
     6661 ggcaatggca acaacattac gcaaactatt aactggcgaa ctacttactc tggcttcacg
     6721 gcaacaatta atagactggc ttgaagcgga taaagttgca ggaccactac tgcgttcggc
     6781 acttcctgct ggctggttta ttgctgataa atctggggca ggagagcgtg gttcacgggg
     6841 tatcattgcc gcacttggac cagatggtaa gccttcccgt atcgtagtta tctacacgac
     6901 gggtagtcag gcaactatgg acgaacgaaa tagacagatt gctgaaatag gggcttcact
     6961 gattaagcat tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac
     7021 ggaccggtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7081 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7141 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7201 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7261 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7321 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7381 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7441 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7501 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7561 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7621 gggcggagcc tatggaaa
//
