LOCUS       sequence-445906-        7481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN and GvpJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNJ
FEATURES             Location/Qualifiers
     source          1..7481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2319..2336
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5740..5758)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        5867..5958
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6823..6862
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6893..7481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7382..7401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gaggagcttg aaaacatgag tgaaacaaac gaaacaggta tttatatttt tagcgccatt
     1801 caaacggata aagacgaaga atttggcgcc gtggaagtag aaggaacaaa agctgaaaca
     1861 tttttgattc gctacaaaga cgcggctatg gtagcagctg aagtaccgat gaaaatttat
     1921 catcctaatc gccaaaattt attaatgcat caaaacgcag tagcagcgat tatggacaag
     1981 aacgatacgg ttattccaat cagctttggg aatgtattca aatcaaaaga agacgtaaaa
     2041 gttcttttgg aaaaccttta tccgcagttt gaaaagctgt ttccagcgat caaaggaaaa
     2101 attgaagtcg gtttaaaagt aattgggaaa aaagaatggc ttgagaaaaa agtaaacgaa
     2161 aatcctgaac ttgagaaagt atcagcatcc gtaaaaggaa aatcagaagc agccggttat
     2221 tatgagcgta ttcaacttgg aggaatggct caaaagatgt ttacttccct gcaaaaagaa
     2281 gtcaagacag atgtgttttc tccgcttgaa gaagcagcgg aagcagcaaa agcaaatgag
     2341 ccaacgggcg aaacgatgct tttaaacgcg tctttcttaa ttaaccgaga agatgaagcg
     2401 aagtttgatg aaaaagtaaa tgaagcgcat gaaaactgga aagacaaagc cgattttcat
     2461 tacagcggtc cttggcctgc ttataatttt gtgaacattc gcctaaaagt agaagagaaa
     2521 taacgtgctt cacaaattag taaccgcacc cattaacctt gtagtgaaaa tcggcgaaaa
     2581 agtacaggaa gaagctgata aacagctata tgaccttccg acgattcagc aaaagctcat
     2641 tcagcttcaa atgatgtttg agcttggtga aattccagaa gaagcgtttc aagaaaaaga
     2701 agatgaattg ttaatgaggt acgaaattgc gaaacgcaga gaaattgaac aatgggaaga
     2761 gctaacacaa aaaagaaatg aggaatccta gatgggagaa ttactgtatt tatacggttt
     2821 aattccaaca aaagaagcag cagccataga gccgtttcca tcttataagg ggtttgacgg
     2881 agaacattca ctgtacccaa ttgcgtttga tcaggtgacg gctgtagttt ctaagctgga
     2941 tgctgacacc tattcagaaa aagtgattca agaaaaaatg gagcaggata tgagctggct
     3001 gcaggaaaaa gcatttcatc atcacgaaac ggtagccgct ttgtacgaag aatttacgat
     3061 cattccatta aaattttgca ccatttataa aggtgaagaa agtctgcagg cagctattga
     3121 gattaacaaa gaaaagatag agaattcact gacgctgctt caaggaaatg aagagtggaa
     3181 tgtgaaaatt tactgtgatg atacagagct taaaaaagga atcagcgaaa cgaatgaaag
     3241 cgtgaaagcg aaaaaacaag aaattagtca cttatcacca ggaagacagt tttttgaaaa
     3301 gaaaaaaata gatcagctga ttgaaaaaga attagagctt cacaaaaaca aagtgtgtga
     3361 agagatacat gacaagctaa aagaattatc gctttatgac tctgttaaaa agaattggag
     3421 caaagacgta actggcgcag ctgaacagat ggcgtggaac agcgtgtttc ttctcccgtc
     3481 tctgcagatt actaagttcg taaacgaaat agaagagctt cagcaaaggc ttgaaaataa
     3541 aggctggaag tttgaagtga cgggaccatg gccgccctat catttctcga gctttgcgta
     3601 aagtgaggaa ttaacattat gtctcttaaa caatccatgg agaataaaga tattgctctt
     3661 attgatattt tagatgtcat tttagataaa ggagtcgcca ttaaaggaga cttaatcatt
     3721 tccatagctg gcgtcgattt agtgtatttg gatttgcggg tgcttatttc ttcggttgaa
     3781 acgcttgtgc aagcaaaaga aggaaatcac aaaccaatca cttctgaaca atttgataaa
     3841 caaaaggagg aattaatgga tgcaaccggt cagccaagca aatggacgaa tccacttgga
     3901 tcctgatcaa gctgaacaag gcttagcgca gcttgtgatg acagttattg agctattgag
     3961 gcaaatcgtt gaacgtcatg ccatgaggcg ggtggagggt ggaacgttga cggacgaaca
     4021 aattgaaaac ttaggaattg cactaatgaa cttagaagaa aaaatggacg agttgaaaga
     4081 ggtgttcggt ctggatgcag aagatttaaa tattgatctt ggaccgctag gcagcctgct
     4141 ttaagcggaa atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc
     4201 agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc
     4261 aagcaaaaca acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc
     4321 aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac
     4381 agacgaatta aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aatcaaaaag
     4441 cagcgtggcc agcctgaaaa catctgcggg aagcttgttt aaaaaagata aagataaatc
     4501 aaaagatgat gaagaaaacg taaattcttc tagtagcgaa acagaagacg ataacgttca
     4561 agagtacgac gagttaaaag aagaaaatca aactcttcaa gatcgcttat cacagcttga
     4621 agaaaaaatg aacatgcttg ttgagcttag cctcaataaa aatcaagacg aagaagcgga
     4681 agatacagat tccgacgaag aagagaacga tgagaacgat gaaaacgatg aaaacgagca
     4741 ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa aaggataaaa aagaagctga
     4801 ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa
     4861 caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa
     4921 aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa
     4981 aaacacggac gaagaagatg atgaagcaac atctctttct agtgaagacg atacaacagc
     5041 ctaagacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac
     5101 aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca
     5161 ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat
     5221 tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc
     5281 tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg
     5341 aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt
     5401 tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa
     5461 tcaacgagta aaaagagttc ataaaaaacg gcggggtgat tgccccgccg ttttttagtg
     5521 atgtgatgag atgtgcagct tcttttttcg atacatgagc ggagtcatct tcatttcttt
     5581 tcgaaactga ttaataaaat agcttgtact attaaaccct acttgatagg cgacttccgt
     5641 cacatttgct tctgcttgct gcagtccgga ttcgcgaacg cgtctcgaga gatccggctg
     5701 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     5761 aaccccttgg ggcctctaaa cgggtcttga ggggtttttg ctgaaaggag gaactatatc
     5821 cgactagtag cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc
     5881 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5941 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6001 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tcgtgaaagt aaaagacgca
     6061 gaggaccaat tgggggcacg agtgggatac atagaactgg acttgaatag cggtaaaatc
     6121 cttgagagtt ttcgccctga agagcgtttt ccaatgatga gcactttcaa agttctgcta
     6181 tgtggagcag tattatcccg tgtagatgcg gggcaagagc aactcggacg acgaatacac
     6241 tattcgcaga atgacttggt tgaatactcc ccagtgacag aaaagcacct tacggacgga
     6301 atgacggtaa gagaattatg tagtgccgcc ataacgatga gtgataacac tgcggcgaac
     6361 ttacttctga caaccatcgg tggaccgaag gaattaaccg cttttttgca caatatggga
     6421 gaccatgtaa ctcgccttga ccgttgggaa ccagaactga atgaagccat accaaacgac
     6481 gagcgagaca ccacaatgcc tgcggcaatg gcaacaacat tacgcaaact attaactggc
     6541 gaactactta ctctggcttc acggcaacaa ttaatagact ggcttgaagc ggataaagtt
     6601 gcaggaccac tactgcgttc ggcacttcct gctggctggt ttattgctga taaatctggg
     6661 gcaggagagc gtggttcacg gggtatcatt gccgcacttg gaccagatgg taagccttcc
     6721 cgtatcgtag ttatctacac gacgggtagt caggcaacta tggacgaacg aaatagacag
     6781 attgctgaaa taggggcttc actgattaag cattggtaaa ccgatacaat taaaggctcc
     6841 ttttggagcc tttttttttg gacggaccgg tagaaaagat caaaggatct tcttgagatc
     6901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6961 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7021 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa a
//
