LOCUS       sequence-445907-        6916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpN and GvpT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaNT
FEATURES             Location/Qualifiers
     source          1..6916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2319..2336
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5175..5193)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        5302..5393
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6258..6297
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6328..6916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6817..6836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gaggagcttg aaaacatgag tgaaacaaac gaaacaggta tttatatttt tagcgccatt
     1801 caaacggata aagacgaaga atttggcgcc gtggaagtag aaggaacaaa agctgaaaca
     1861 tttttgattc gctacaaaga cgcggctatg gtagcagctg aagtaccgat gaaaatttat
     1921 catcctaatc gccaaaattt attaatgcat caaaacgcag tagcagcgat tatggacaag
     1981 aacgatacgg ttattccaat cagctttggg aatgtattca aatcaaaaga agacgtaaaa
     2041 gttcttttgg aaaaccttta tccgcagttt gaaaagctgt ttccagcgat caaaggaaaa
     2101 attgaagtcg gtttaaaagt aattgggaaa aaagaatggc ttgagaaaaa agtaaacgaa
     2161 aatcctgaac ttgagaaagt atcagcatcc gtaaaaggaa aatcagaagc agccggttat
     2221 tatgagcgta ttcaacttgg aggaatggct caaaagatgt ttacttccct gcaaaaagaa
     2281 gtcaagacag atgtgttttc tccgcttgaa gaagcagcgg aagcagcaaa agcaaatgag
     2341 ccaacgggcg aaacgatgct tttaaacgcg tctttcttaa ttaaccgaga agatgaagcg
     2401 aagtttgatg aaaaagtaaa tgaagcgcat gaaaactgga aagacaaagc cgattttcat
     2461 tacagcggtc cttggcctgc ttataatttt gtgaacattc gcctaaaagt agaagagaaa
     2521 taacgtgctt cacaaattag taaccgcacc cattaacctt gtagtgaaaa tcggcgaaaa
     2581 agtacaggaa gaagctgata aacagctata tgaccttccg acgattcagc aaaagctcat
     2641 tcagcttcaa atgatgtttg agcttggtga aattccagaa gaagcgtttc aagaaaaaga
     2701 agatgaattg ttaatgaggt acgaaattgc gaaacgcaga gaaattgaac aatgggaaga
     2761 gctaacacaa aaaagaaatg aggaatccta gatgggagaa ttactgtatt tatacggttt
     2821 aattccaaca aaagaagcag cagccataga gccgtttcca tcttataagg ggtttgacgg
     2881 agaacattca ctgtacccaa ttgcgtttga tcaggtgacg gctgtagttt ctaagctgga
     2941 tgctgacacc tattcagaaa aagtgattca agaaaaaatg gagcaggata tgagctggct
     3001 gcaggaaaaa gcatttcatc atcacgaaac ggtagccgct ttgtacgaag aatttacgat
     3061 cattccatta aaattttgca ccatttataa aggtgaagaa agtctgcagg cagctattga
     3121 gattaacaaa gaaaagatag agaattcact gacgctgctt caaggaaatg aagagtggaa
     3181 tgtgaaaatt tactgtgatg atacagagct taaaaaagga atcagcgaaa cgaatgaaag
     3241 cgtgaaagcg aaaaaacaag aaattagtca cttatcacca ggaagacagt tttttgaaaa
     3301 gaaaaaaata gatcagctga ttgaaaaaga attagagctt cacaaaaaca aagtgtgtga
     3361 agagatacat gacaagctaa aagaattatc gctttatgac tctgttaaaa agaattggag
     3421 caaagacgta actggcgcag ctgaacagat ggcgtggaac agcgtgtttc ttctcccgtc
     3481 tctgcagatt actaagttcg taaacgaaat agaagagctt cagcaaaggc ttgaaaataa
     3541 aggctggaag tttgaagtga cgggaccatg gccgccctat catttctcga gctttgcgta
     3601 aagtgaggaa ttaacattat gtctcttaaa caatccatgg agaataaaga tattgctctt
     3661 attgatattt tagatgtcat tttagataaa ggagtcgcca ttaaaggaga cttaatcatt
     3721 tccatagctg gcgtcgattt agtgtatttg gatttgcggg tgcttatttc ttcggttgaa
     3781 acgcttgtgc aagcaaaaga aggaaatcac aaaccaatca cttctgaaca atttgataaa
     3841 caaaaggagg aattaatgga tgcaaccggt cagccaagca aatggacgaa tccacttgga
     3901 tcctgatcaa gctgaacaag gcttagcgca gcttgtgatg acagttattg agctattgag
     3961 gcaaatcgtt gaacgtcatg ccatgaggcg ggtggagggt ggaacgttga cggacgaaca
     4021 aattgaaaac ttaggaattg cactaatgaa cttagaagaa aaaatggacg agttgaaaga
     4081 ggtgttcggt ctggatgcag aagatttaaa tattgatctt ggaccgctag gcagcctgct
     4141 ttaagcggtc agtaggagga acagtatggc agtcgaacat aatatgcagt caagtacgat
     4201 tgtagatgtg ctcgaaaaga ttttggataa aggagtcgtt atagcggggg acatcaccgt
     4261 aggaattgca gatgtcgagc tattaacgat aaagatccgc ttgattgtgg cttcggttga
     4321 taaggcaaaa gaaatcggca tggactggtg ggaaaatgat ccgtatctca gttcaaaagg
     4381 agccaataac aaagcgctcg aagaagaaaa taaaatgctg catgagcggt taaaaacgct
     4441 tgaagaaaaa atagaaacga aacgttaaaa actgtacgct acttaaagga ggaaagaaag
     4501 acatgagtac aggcccttct ttttcaacta aagacaatac gcttgaatac tttgtgaaag
     4561 cttctaataa acacggcttt tcacttgata tttcattaaa tgtaaacggc gctgtgattt
     4621 ccggtaccat gatttcagca aaagaatact ttgattactt aagcgaaacg tttgaagaag
     4681 gcagtgaagt ggctcaggcg ctaagcgaac aattctcttt agcaagcgaa gcgagcgaat
     4741 caaacggaga agcagaagcc cattttattc atttgaaaaa tacaaagatt tactgtggag
     4801 acagtaaatc tactccttct aaaggcaaaa tcttttggag agggaaaata gcagaagtag
     4861 acgggttttt cttaggaaag atttctgatg caaaatcaac gagtaaaaag agttcataaa
     4921 aaacggcggg gtgattgccc cgccgttttt tagtgatgtg atgagatgtg cagcttcttt
     4981 tttcgataca tgagcggagt catcttcatt tcttttcgaa actgattaat aaaatagctt
     5041 gtactattaa accctacttg ataggcgact tccgtcacat ttgcttctgc ttgctgcagt
     5101 ccggattcgc gaacgcgtct cgagagatcc ggctgctaac aaagcccgaa aggaagctga
     5161 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     5221 cttgaggggt ttttgctgaa aggaggaact atatccgact agtagcccgc ctaatgagcg
     5281 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5461 ctcacccaga aacgctcgtg aaagtaaaag acgcagagga ccaattgggg gcacgagtgg
     5521 gatacataga actggacttg aatagcggta aaatccttga gagttttcgc cctgaagagc
     5581 gttttccaat gatgagcact ttcaaagtta tgctatgtgg agcagtatta tcccgtgtag
     5641 atgcggggca agagcaactc ggacgacgaa tacactattc gcagaatgac ttggttgaat
     5701 actccccagt gacagaaaag caccttacgg acggaatgac ggtaagagaa ttatgtagtg
     5761 ccgccataac gatgagtgat aacactgcgg cgaacttact tctgacaacc atcggtggac
     5821 cgaaggaatt aaccgctttt ttgcacaata tgggagacca tgtaactcgc cttgaccgtt
     5881 gggaaccaga actgaatgaa gccataccaa acgacgagcg agacaccaca atgcctgcgg
     5941 caatggcaac aacattacgc aaactattaa ctggcgaact acttactctg gcttcacggc
     6001 aacaattaat agactggctt gaagcggata aagttgcagg accactactg cgttcggcac
     6061 ttcctgctgg ctggtttatt gctgataaat ctggggcagg agagcgtggt tcacggggta
     6121 tcattgccgc acttggacca gatggtaagc cttcccgtat cgtagttatc tacacgacgg
     6181 gtagtcaggc aactatggac gaacgaaata gacagattgc tgaaataggg gcttcactga
     6241 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacgg
     6301 accggtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6481 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6541 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6601 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6901 gcggagccta tggaaa
//
