LOCUS       sequence-445913-        4462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with Dok1 tagged with Clover.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pENTR2b-Dok1-Clover
FEATURES             Location/Qualifiers
     source          1..4462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(1959..1961,1962..1964,1965..2675)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2004..2025)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2612..2633
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2691..2790)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2902..2921)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2913..3722
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2980..2999)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3812..4400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4301..4320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccgg taccggcacc atggacggag cagtgatgga agggccgctt tttttgcaga
      541 gtcagcgctt tgggaccaag aggtggagga agacctgggc cgtgctctac ccggccagtc
      601 cccacggcgt agcgcggctc gagttctttg accataaggg gtcgagctct gggggtggcc
      661 gagggagctc gcgccgcctg gactgcaaag tgatccgtct ggctgagtgt gtgagtgtgg
      721 cccccgtcac cgtggagacc ccccctgagc ccggcgccac tgccttccgc ctggacactg
      781 ctcagcgctc gcacctgctg gcggccgacg cgccgtccag tgcagcctgg gtgcagacgc
      841 tgtgccgaaa cgcctttccg aaaggcagct ggactctggc gcctaccgat aacccaccta
      901 agctttctgc cctggagatg ctggagaact ccttgtacag ccctacctgg gaaggatccc
      961 aattctgggt aacggtgcag aggactgagg ccgccgagcg ctgtggcctg catggctcct
     1021 acgtgctgag ggtggaggct gaaaggctga ctctcctgac cgtgggggcc cagagtcaga
     1081 tactggagcc actcctgtcc tggccctaca ctctgttgcg tcgctatggc cgggacaagg
     1141 tcatgttctc tttcgaggcc ggccgccgct gcccctcagg ccctggaacc ttcaccttcc
     1201 agacggcaca gggaaatgac atcttccagg cagttgagac tgccatccac cggcagaagg
     1261 cccagggaaa ggccggacag gggcacgatg ttctcagagc tgactcccat gaaggggagg
     1321 tggcagaggg gaagttgcct tccccacctg gcccccaaga gctcctcgac agtcccccag
     1381 ccctgtatgc tgagccctta gactccctgc gcattgctcc atgcccttcc caggactccc
     1441 tatactcaga ccccttggac agcacgtctg ctcaggcagg agagggagta caacggaaga
     1501 aacctctcta ttgggacttg tatgagcatg cgcagcagca gttgctgaag gccaagctga
     1561 cagaccccaa agaggatccc atctatgatg aacctgaggg cctggcccca gtccctcccc
     1621 agggccttta tgatctgcct cgggagccca aggatgcatg gtggtgccaa gctcgggtga
     1681 aggaggaggg ctatgagctc ccctacaacc ctgccactga tgactacgct gtgccacccc
     1741 ctcggagcac aaagcccctc cttgctccca agccccaggg cccagccttc cctgaacctg
     1801 gtactgcaac tggcagtggc atcaaaagcc acaactcagc cctgtacagc caggtccaga
     1861 agagcggggc ctcagggagc tgggactgtg ggctctctag agtagggact gacaagactg
     1921 gggtcaagtc agagggctct accggcggcg gcggccgcat ggtgagcaag ggcgaggagc
     1981 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     2041 tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
     2101 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
     2161 gcgtggcctg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2221 ccatgcccga aggctacgtc caggagcgca ccatctcttt caaggacgac ggtacctaca
     2281 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2341 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
     2401 gccacaacgt ctatatcacg gccgacaagc agaagaacgg catcaaggct aacttcaaga
     2461 tccgccacaa cgttgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2521 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat cagtccgccc
     2581 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2641 ccgggattac acatggcatg gacgagctgt acaagtagta agatatctag acccagcttt
     2701 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2761 cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc
     2821 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     2881 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     2941 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     3001 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     3061 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     3121 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     3181 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     3241 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     3301 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     3361 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     3421 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     3481 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     3541 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     3601 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     3661 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     3721 aatcagaatt ggttaattgg ttgtaacatt attcagattg ggccccgttc cactgagcgt
     3781 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3841 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3901 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3961 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4021 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4081 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4141 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4201 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4261 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4321 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4381 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4441 gctggccttt tgctcacatg tt
//
