LOCUS       sequence-44593-e         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Gupta RK, Arany Z, Seale P, Mepani RJ, Ye L, Conroe HM, Roby YA, 
            Kulaga H, Reed RR, Spiegelman BM
  TITLE     Transcriptional control of preadipocyte determination by Zfp423.
  JOURNAL   Nature. 2010 Mar 25;464(7288):619-23. doi: 10.1038/nature08816. Epub
            2010 Mar 3.
  PUBMED    20200519
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08816"; journalName: "Nature"; date: "2010-03-25- 
            25"; volume: "464"; issue: "7288"; pages: "619-23"
COMMENT     Sequence Label: pMKO.1-Zfp423
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..306
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     58..78
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     229..248
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(391..411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattga agatctgtcg acaaggtcgg gcaggaagag
       61 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      121 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      181 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      241 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      301 aacaccggcc ctgaatgtaa cgtgaagttc tcgagaactt cacgttacat tcagggtttt
      361 tgaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
      421 ccccaggctt cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      541 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcatag gccgaggccg
      661 cctcggcctc tgagctattc c
//
