LOCUS       sequence-445943-        9911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Landing Pad donor vector containing partial RdRp and N gene 
            sequences from SARS-CoV-2 for fluorescent reporter assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Wan Y, Helenek C, Coraci D, Balazsi G
  TITLE     Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA 
            Downregulation with Minimal Collateral RNA Cutting.
  JOURNAL   ACS Synth Biol. 2024 Oct 18;13(10):3212-3230. doi: 
            10.1021/acssynbio.4c00271. Epub 2024 Oct 8.
  PUBMED    39377757
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00271"; journalName: "ACS Synth Biol"; date: 
            "2024-10-18- 18"; volume: "13"; issue: "10"; pages: "3212-3230"
COMMENT     Sequence Label: LPUtopia-7_CoV-2
FEATURES             Location/Qualifiers
     source          1..9911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2074..2662
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2127..2147)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2545..2556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2665..2681)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2666..2682)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2704..2721)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2710..2934
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3095..3683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3175..3194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3854..4645,4646..4714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4477..4496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4715..4819)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4845..5300)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4982..5003)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5194..5213
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5427..5449
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5441..5458
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5442..5458
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5588..5650
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    6113..6916
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    6959..6992
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(7013..7046)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7331..8134
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7394..7413)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8004..8023
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8141..8189
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(8200..8233)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8302..8321)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8493..8872
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8873..9076
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9027..9047
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9121..9140
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9121..9139
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9178..9187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9184..9186,9187..9189,9190..9903)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9184..9186,9187..9189,9190..9900)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9229..9250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9490..9509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9837..9858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgactctaga gggccctatt atggtcatgt gtggcggttc actatatgtt aaaccaggtg
       61 gaacctcatc aggagatgcc acaactgctt atgctaatag tgtttttaac atttgtcaag
      121 ctgtcacggc caatgttaat gcacttttat ctactgatgg taacaaaatt gccgataagt
      181 atgtccgcaa tttacaacac agactttatg agtgtctcta tagaaataga gatgttgaca
      241 cagactttgt gaatgagttt tacgcatatt tgcgtaaaca tttctcaatg atgatactct
      301 ctgacgatgc tgttgtgtgt ttcaatagca cttatgcatc tcaaggtcta gtggctagca
      361 taaagaactt taagtcagtt ctttattatc aaaacaatgt ttttatgtct gaagcaaaat
      421 gttggactga gactgacctt actaaaggac ctcatgaatt ttgctctcaa catacaatgc
      481 tagttaaaca gggtgatgat tatgtgtacc ttccttaccc agatccatca agaatcctag
      541 gggccggctg ttttgtagat gatatcgtaa aaacagatgg tacacttatg attgaacggt
      601 tcgtgtcttt agctatagat gcttacccac ttactaaaca tcctaatcag gagtatgctg
      661 atgtctttca tttgtactta caatacataa gaaagctaca tgatgagtta acaggacaca
      721 tgttagacat gtattctgtt atgcttacta atgataacac ttcaaggtat tgggaacctg
      781 agttttatgt ctgataatgg ccccaaaatc agcgaaatgc accccgcatt acgtttggtg
      841 gaccctcaga ttcaactggc agtaaccaga atggagaacg cagtggggcg cgatcaaaac
      901 aacgtcggcc ccaaggttta cccaataata ctgcgtcttg gttcaccgct ctcactcaac
      961 atggcaagga agaccttaaa ttccctcgag gacaaggcgt tccaattaac accaatagca
     1021 gtccagatga ccaaattggc tactaccgaa gagctaccag acgaattcgt ggtggtgacg
     1081 gtaaaatgaa agatctcagt ccaagatggt atttctacta cctaggaact gggccagaag
     1141 ctggacttcc ctatggtgct aacaaagacg gcatcatatg ggttgcaact gagggagcct
     1201 tgaatacacc aaaagatcac attggcaccc gcaatcctgc taacaatgct gcaatcgtgc
     1261 tacaacttcc tcaaggaaca acattgccaa aaggcttcta cgcagaaggg agcagaggcg
     1321 gcagtcaagc ctcttctcgt tcctcatcac gtagtcgcaa cagttcaaga aattcaactc
     1381 caggcagcag taggggaact tctcctgcta gaatggctgg caatggcggt gatgctgctc
     1441 ttgctttgct gctgcttgac agattgaacc agcttgagag caaaatgtct ggtaaaggcc
     1501 aacaacaaca aggccaaact gtcactaaga aatctgctgc tgaggcttct aagaagcctc
     1561 ggcaaaaacg tactgccact aaagcataca atgtaacaca agctttcggc agacgtggtc
     1621 cagaacaaac ccaaggaaat tttggggacc aggaactaat cagacaagga actgattaca
     1681 aacattggcc gcaaattgca caatttgccc ccagcgcttc agcgttcttc ggaatgtcgc
     1741 gcattggcat ggaagtcaca ccttcgggaa cgtggttgac ctacacaggt gccatcaaat
     1801 tggatgacaa agatccaaat ttcaaagatc aagtcatttt gctgaataag catattgacg
     1861 catacaaaac attcccacca acagagccta aaaaggacaa aaagaagaag gctgatgaaa
     1921 ctcaagcctt accgcagaga cagaagaaac agcaaactgt gactcttctt cctgctgcag
     1981 atttggatga tttctccaaa caattgcaac aatccatgag cagtgctgac tcaactcagg
     2041 ccggcatgca agcttgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2101 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2161 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2221 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2281 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2341 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2401 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2461 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2521 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2581 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2641 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gaatgctaga gctcgctgat
     2701 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2761 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2821 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2881 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggagatct
     2941 gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
     3001 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaaagg
     3061 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     4861 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     4921 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     4981 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5041 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5101 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5161 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5221 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5281 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5341 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     5401 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     5461 gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac gggcagactc gcggtgcaaa
     5521 tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgcagct
     5581 agattaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc atgtgtaaaa
     5641 ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat
     5701 taagttttat tatatttaca cttacatact aataataaat tcaacaaaca atttatttat
     5761 gtttatttat ttattaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact
     5821 tttatgaggg acagcccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     5881 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     5941 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     6001 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc
     6061 tccacggcca ctagtaacgg ccgccagtgt gctggaattc gcccttggta cctgctttct
     6121 ctgaccagca ttctctcccc tgggcctgtg ccgctttctg tctgcagctt gtggcctggg
     6181 tcacctctac ggctggccca gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc
     6241 actccctctt ccccttgctc tctgctgtgt tgctgcccaa ggatgctctt tccggagcac
     6301 ttccttctcg gcgctgcacc acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc
     6361 ctctccgggc atctctcctc cctcacccaa ccccatgccg tcttcactcg ctgggttccc
     6421 ttttccttct ccttctgggg cctgtgccat ctctcgtttc ttaggatggc cttctccgac
     6481 ggatgtctcc cttgcgtccc gcctcccctt cttgtaggcc tgcatcatca ccgtttttct
     6541 ggacaacccc aaagtacccc gtctccctgg ctttagccac ctctccatcc tcttgctttc
     6601 tttgcctgga caccccgttc tcctgtggat tcgggtcacc tctcactcct ttcatttggg
     6661 cagctcccct acccccctta cctctctagt ctgtgctagc tcttccagcc ccctgtcatg
     6721 gcatcttcca ggggtccgag agctcagcta gtcttcttcc tccaacccgg gcccctatgt
     6781 ccacttcagg acagcatgtt tgctgcctcc agggatcctg tgtccccgag ctgggaccac
     6841 cttatattcc cagggccggt taatgtggct ctggttctgg gtacttttat ctgtcccctc
     6901 caccccacag tggggcacgc gtaagggcga attctgcaga tatcgaagtt cctattccga
     6961 agttcctatt cttcaaatag tataggaact tcgtcgagat cgatggcgcg ccataacttc
     7021 gtatagcata cattatacga agttatgaat ttcgagcagt gtggttttca agaggaagca
     7081 aaaagcctct ccacccaggc ctggaatgtt tccacccaat gtcgagcagt gtggttttgc
     7141 aagaggaagc aaaaagcctc tccacccagg cctggaatgt ttccacccaa tgtcgagcaa
     7201 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     7261 ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     7321 tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc
     7381 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7441 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7501 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7561 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7621 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7681 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7741 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7801 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7861 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc
     7921 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7981 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     8041 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     8101 cgccttctat cgccttcttg acgagttctt ctgaggggat cggcaataaa aagacagaat
     8161 aaaacgcacg ggtgttgggt cgtttgttcg gatcaattca taacttcgta tagcatacat
     8221 tatacgaagt tatggcgcgc cttaattaag gatccgtcga cggatcggga gatctcccga
     8281 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     8341 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     8401 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     8461 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     8521 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     8581 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     8641 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     8701 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     8761 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     8821 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     8881 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     8941 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     9001 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     9061 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     9121 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc accggtcgcc
     9181 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     9241 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     9301 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     9361 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     9421 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     9481 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     9541 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     9601 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     9661 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     9721 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     9781 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     9841 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     9901 taaagcggcc g
//
