LOCUS       sequence-445949-        8913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Landing Pad donor vector for AAVS1 site integration in human and 
            VeroE6 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Wan Y, Helenek C, Coraci D, Balazsi G
  TITLE     Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA 
            Downregulation with Minimal Collateral RNA Cutting.
  JOURNAL   ACS Synth Biol. 2024 Oct 18;13(10):3212-3230. doi: 
            10.1021/acssynbio.4c00271. Epub 2024 Oct 8.
  PUBMED    39377757
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00271"; journalName: "ACS Synth Biol"; date: 
            "2024-10-18- 18"; volume: "13"; issue: "10"; pages: "3212-3230"
COMMENT     Sequence Label: LPUtopia-8
FEATURES             Location/Qualifiers
     source          1..8913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(37..70)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    113..916
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     repeat_region   1379..1441
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1571..1588)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1571..1587)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1580..1602)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1729..2184
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1816..1835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2026..2047
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2210..2314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2315..2383,2384..3175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2533..2552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3346..3934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3835..3854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4095..4319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5088..5112)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5109..5312)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     repeat_region   5539..5573
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    5583..6419
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(6809..6856)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    6977..7088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7077..7094
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(7242..7841)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7325..7345)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7822..7841
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(7851..7904)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(7905..8615,8616..8618,8619..8621))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7905..8615,8616..8618,8619..8621))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7947..7968)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8296..8315
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      8618..8627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8665..8684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8666..8684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(8729..8913,1..19))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 actgccaaaa ccgcatcacg cgccatcgat ctcgacgaag ttcctatact atttgaagaa
       61 taggaacttc ggaataggaa cttcgatatc tgcagaattc gcccttacgc gtgccccact
      121 gtggggtgga ggggacagat aaaagtaccc agaaccagag ccacattaac cggccctggg
      181 aatataaggt ggtcccagct cggggacaca ggatccctgg aggcagcaaa catgctgtcc
      241 tgaagtggac ataggggccc gggttggagg aagaagacta gctgagctct cggacccctg
      301 gaagatgcca tgacaggggg ctggaagagc tagcacagac tagagaggta aggggggtag
      361 gggagctgcc caaatgaaag gagtgagagg tgacccgaat ccacaggaga acggggtgtc
      421 caggcaaaga aagcaagagg atggagaggt ggctaaagcc agggagacgg ggtactttgg
      481 ggttgtccag aaaaacggtg atgatgcagg cctacaagaa ggggaggcgg gacgcaaggg
      541 agacatccgt cggagaaggc catcctaaga aacgagagat ggcacaggcc ccagaaggag
      601 aaggaaaagg gaacccagcg agtgaagacg gcatggggtt gggtgaggga ggagagatgc
      661 ccggagagga cccagacacg gggaggatcc gctcagagga catcacgtgg tgcagcgccg
      721 agaaggaagt gctccggaaa gagcatcctt gggcagcaac acagcagaga gcaaggggaa
      781 gagggagtgg aggaagacgg aacctgaagg aggcggcagg gaaggatctg ggccagccgt
      841 agaggtgacc caggccacaa gctgcagaca gaaagcggca caggcccagg ggagagaatg
      901 ctggtcagag aaagcaggta ccaagggcga attccagcac actggcggcc gttactagtg
      961 gccgtggagg ccttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
     1021 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
     1081 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
     1141 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg ggggctgtcc
     1201 ctcataaaag ttttgttact ttatagaaga aattttgagt ttttgttttt tttaataaat
     1261 aaataaacat aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat
     1321 aaaacttaat atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca
     1381 tgcgtcaatt ttacacatga ttatctttaa cgtacgtcac aatatgatta tctttctagg
     1441 gttaatctag ctgcgtgttc tgcagcgtgt cgagcatctt catctgctcc atcacgctgt
     1501 aaaacacatt tgcaccgcga gtctgcccgt cctccacggg ttcaaaaacg tgaatgaacg
     1561 aggcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1621 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1681 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     1741 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1801 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1861 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1921 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1981 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2041 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2101 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2161 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2221 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2401 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2461 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2521 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2581 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2641 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2701 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2761 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2821 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2881 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2941 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3001 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3061 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3301 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3361 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3421 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3481 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3541 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3601 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3661 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3721 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3781 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3841 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3901 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3961 gttcctggcc ttttctctag gcacccgttc aattgccgac ccctcccccc aacttctcgg
     4021 ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagcgatcg cagatccttc
     4081 gcggccgcag atctccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     4141 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     4201 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     4261 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagg
     4321 gatccgattt aaattcgaat taccctggca gggcgctacg ggatgccacg gcgtgctcga
     4381 ccagttgcca cttctggttc ttggcatctg agcggtcctc gcgggtgagt ttgtgctgga
     4441 tgaagtgcca ctctggcatc tccttgggat cggactttgc cttgtagatg gtatcgaact
     4501 ggcatcggta tcttcccccg tccttgagca ggaggtacat tgcgatgtcg cccttcaaga
     4561 ttccctgacg agggaccggg atgattttct cgcagctggg ctcccaattc gtagtcattt
     4621 tcttcatcac aggcccgtcg gcaggaaagt tcacgccgtg gaatttgctg ttgtggtaga
     4681 agcagttctc ctcgacggac acggtgatat cagcggaggt ggtacacacc gccccgtcct
     4741 cgaagagcag gctgcggtgc caggtgtatc ctgcgggaca gctgttctta aagaaatcca
     4801 ccataccctg tggatactct gtgaacacgc gattgccgta gtcaaaggtt gcggacagga
     4861 tgtcctccga gaagggcagg ggtccgcctt ccaccacaca caggttcatt gtctgtttcc
     4921 cctcgaatgg gttgccgttc ccgtggcccg taatcacgaa cttgtgcccg tccaccgagc
     4981 cctccatatg gtacttcatg gtcatgtcct tagtcaggcc gtgcttacta agggccattc
     5041 tagaatcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc
     5101 actaaacgag ctctgcttat ataaacctcc caccgtacac gcctaccgcc catttgcgtc
     5161 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     5221 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     5281 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     5341 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatt catcctcggc
     5401 aaactctttc catgccgctt caacctcgcg ggaaaaggca cgggcttctt cctccccgat
     5461 gcccagatag cgccagcttg ggcgatgact gagccggaaa aaagacccga cgatatgatc
     5521 ctgatgcagc tagattaacc ctagaaagat agtctgcgta aaattgacgc atgcatctcg
     5581 agagagcaga gccaggaaca cctgtaggga aggggcagga gagccagggg catgagatgg
     5641 tggacgagga agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa
     5701 acccagaagc ccagagcagg gccttaggga agcgggaccc tgctctgggc ggaggaatat
     5761 gtcccagata gcactgggga ctctttaagg aaagaaggat ggagaaagag aaagggagta
     5821 gaggcggcca cgacctggtg aacacctagg acgcaccatt ctcacaaagg gagttttcca
     5881 cacggacacc cccctcctca ccacagccct gccaggacgg ggctggctac tggccttatc
     5941 tcacaggtaa aactgacgca cggaggaaca atataaattg gggactagaa aggtgaagag
     6001 ccaaagttag aactcaggac caacttattc tgattttgtt tttccaaact gcttctcctc
     6061 ttgggaagtg taaggaagct gcagcaccag gatcagtgaa acgcaccaga cagccgcgtc
     6121 agagcagctc aggttctggg agagggtagc gcagggtggc cactgagaac cgggcaggtc
     6181 acgcatcccc cccttccctc ccaccccctg ccaagctctc cctcccagga tcctctctgg
     6241 ctccatcgta agcaaacctt agaggttctg gcaaggagag agatggctcc aggaaatggg
     6301 ggtgtgtcac cagataagga atctgcctaa caggaggtgg gggttagacc caatatcagg
     6361 agactaggaa ggaggaggcc taaggatggg gcttttctgt caccaatcct gtccctagta
     6421 gtgtacgtta aagataatca tgcgtaaaat tgacgcatgt gttttatcgg tctgtatatc
     6481 gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac ttacatacta
     6541 ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa aaacaaaaac
     6601 tcaaaatttc ttctataaag taacaaaact tttaaacatt ctctctttta caaaaataaa
     6661 cttattttgt actttaaaaa cagtcatgtt gtattataaa ataagtaatt agcttaacct
     6721 atacataata gaaacaaatt atacttatta gtcagtcaga aacaactttg gcacatatca
     6781 atattatgct ctcgttaatt aactcgagga agttcctata ctttctagag aataggaact
     6841 tcggaatagg aacttcgtcg acaagccata gagcggtggt tacgcgctaa agctgctatt
     6901 gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca
     6961 cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc
     7021 accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga
     7081 aggcacagtc gaggctgatc agcgggttta aacgggccct ctagagtcgg tgggcctcgg
     7141 gggcgggtgc ggggtcggcg gggccgcccc gggtggcttc ggtcggagcc atggggtcgt
     7201 gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg
     7261 gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg
     7321 agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg
     7381 gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg
     7441 tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc
     7501 gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg
     7561 cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc
     7621 gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg
     7681 aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg
     7741 cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg
     7801 tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tttcgagcct agggccggga
     7861 ttctcctcca cgtcaccgca tgttagaaga cttcctctgc cctccttgta cagctcgtcc
     7921 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     7981 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
     8041 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     8101 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     8161 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     8221 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     8281 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     8341 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     8401 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
     8461 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     8521 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     8581 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc
     8641 gagctcggta ccaagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
     8701 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
     8761 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     8821 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     8881 gtgagtcaaa ccgctatcca cgcccattga tgt
//
