LOCUS       sequence-44597-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Nagahara H, Vocero-Akbani AM, Snyder EL, Ho A, Latham DG, Lissy NA, 
            Becker-Hapak M, Ezhevsky SA, Dowdy SF
  TITLE     Transduction of full-length TAT fusion proteins into mammalian 
            cells: TAT-p27Kip1 induces cell migration.
  JOURNAL   Nat Med. 1998 Dec;4(12):1449-52.
  PUBMED    9846587
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "1998-12"; volume: "4(12)"; pages: "1449-52"
COMMENT     Sequence Label: pTAT-HA
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..81
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(85..93,94..108)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             187..213
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(314..332)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      328..375
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(559..578)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     769..790
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat ggctagcatg
       61 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tcgatgggga
      121 tccaagcttg gctacggccg caagaaacgc cgccagcgcc gccgcggtgg atccaccatg
      181 tccggctatc catatgacgt cccagactat gctggctcca tggccggtac cggtctcgag
      241 gtgcatgcgg tgaattcgaa gcttgatccg gctgctaaca aagcccgaaa ggaagctgag
      301 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      361 ttgaggggtt ttttgctgaa aggaggaact atatccggat ctggcgtaat agcgaagagg
      421 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
      481 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      541 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      601 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      661 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
      721 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      781 tccaaactgg aacaacactc aaccctatct cggtctattc t
//
