LOCUS       sequence-445975-        7729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpF and GvpG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaFG
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5987..6005)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6001..6048
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6115..6206
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7071..7110
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7141..7729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7630..7649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaatgag gaatcctaga
     2701 tgggagaatt actgtattta tacggtttaa ttccaacaaa agaagcagca gccatagagc
     2761 cgtttccatc ttataagggg tttgacggag aacattcact gtacccaatt gcgtttgatc
     2821 aggtgacggc tgtagtttct aagctggatg ctgacaccta ttcagaaaaa gtgattcaag
     2881 aaaaaatgga gcaggatatg agctggctgc aggaaaaagc atttcatcat cacgaaacgg
     2941 tagccgcttt gtacgaagaa tttacgatca ttccattaaa attttgcacc atttataaag
     3001 gtgaagaaag tctgcaggca gctattgaga ttaacaaaga aaagatagag aattcactga
     3061 cgctgcttca aggaaatgaa gagtggaatg tgaaaattta ctgtgatgat acagagctta
     3121 aaaaaggaat cagcgaaacg aatgaaagcg tgaaagcgaa aaaacaagaa attagtcact
     3181 tatcaccagg aagacagttt tttgaaaaga aaaaaataga tcagctgatt gaaaaagaat
     3241 tagagcttca caaaaacaaa gtgtgtgaag agatacatga caagctaaaa gaattatcgc
     3301 tttatgactc tgttaaaaag aattggagca aagacgtaac tggcgcagct gaacagatgg
     3361 cgtggaacag cgtgtttctt ctcccgtctc tgcagattac taagttcgta aacgaaatag
     3421 aagagcttca gcaaaggctt gaaaataaag gctggaagtt tgaagtgacg ggaccatggc
     3481 cgccctatca tttctcgagc tttgcgtaaa gtgaggaatt aacattatgt ctcttaaaca
     3541 atccatggag aataaagata ttgctcttat tgatatttta gatgtcattt tagataaagg
     3601 agtcgccatt aaaggagact taatcatttc catagctggc gtcgatttag tgtatttgga
     3661 tttgcgggtg cttatttctt cggttgaaac gcttgtgcaa gcaaaagaag gaaatcacaa
     3721 accaatcact tctgaacaat ttgataaaca aaaggaggaa ttaatggatg caaccggtca
     3781 gccaagcaaa tggacgaatc cacttggatc ctgatcaagc tgaacaaggc ttagcgcagc
     3841 ttgtgatgac agttattgag ctattgaggc aaatcgttga acgtcatgcc atgaggcggg
     3901 tggagggtgg aacgttgacg gacgaacaaa ttgaaaactt aggaattgca ctaatgaact
     3961 tagaagaaaa aatggacgag ttgaaagagg tgttcggtct ggatgcagaa gatttaaata
     4021 ttgatcttgg accgctaggc agcctgcttt aagcggtcag taggaggaac agtatggcag
     4081 tcgaacataa tatgcagtca agtacgattg tagatgtgct cgaaaagatt ttggataaag
     4141 gagtcgttat agcgggggac atcaccgtag gaattgcaga tgtcgagcta ttaacgataa
     4201 agatccgctt gattgtggct tcggttgata aggcaaaaga aatcggcatg gactggtggg
     4261 aaaatgatcc gtatctcagt tcaaaaggag ccaataacaa agcgctcgaa gaagaaaata
     4321 aaatgctgca tgagcggtta aaaacgcttg aagaaaaaat agaaacgaaa cgttaaaaac
     4381 tgtacgctac ttaaaaaatg gagggattta caatggcaac tgaaacaaaa ttagataaca
     4441 cacaggcaga aaacaaggaa aataaaaatg cggaaaacgg ttcaaaagaa aagaacggtt
     4501 caaaagcaag caaaacaaca agcagcgggc caatcaaacg agcggtagca ggaggcatca
     4561 tcggtgcaac gattggatat gtatcgactc ctgaaaatcg aaaaagtctc cttgaccgca
     4621 ttgatacaga cgaattaaaa agcaaagcat ctgatttagg aacaaaggta aaagaaaaat
     4681 caaaaagcag cgtggccagc ctgaaaacat ctgcgggaag cttgtttaaa aaagataaag
     4741 ataaatcaaa agatgatgaa gaaaacgtaa attcttctag tagcgaaaca gaagacgata
     4801 acgttcaaga gtacgacgag ttaaaagaag aaaatcaaac tcttcaagat cgcttatcac
     4861 agcttgaaga aaaaatgaac atgcttgttg agcttagcct caataaaaat caagacgaag
     4921 aagcggaaga tacagattcc gacgaagaag agaacgatga gaacgatgaa aacgatgaaa
     4981 acgagcagga cgatgaaaac gaagaagaaa catctaagcc acgtaaaaag gataaaaaag
     5041 aagctgagga agaagaaagt gaaagtgacg aagacagcga ggaagaagag gaagattctc
     5101 gctcaaacaa aaaaaataaa aaagtaaaaa cagaagaaga agacgaagat gaaagcgaag
     5161 aagaaaaaaa ggaagcgaaa ccaaaaaagt caacagctaa aaaatcaaaa aatacaaaag
     5221 caaagaaaaa cacggacgaa gaagatgatg aagcaacatc tctttctagt gaagacgata
     5281 caacagccta agacgtaaag gaggaaagaa agacatgagt acaggccctt ctttttcaac
     5341 taaagacaat acgcttgaat actttgtgaa agcttctaat aaacacggct tttcacttga
     5401 tatttcatta aatgtaaacg gcgctgtgat ttccggtacc atgatttcag caaaagaata
     5461 ctttgattac ttaagcgaaa cgtttgaaga aggcagtgaa gtggctcagg cgctaagcga
     5521 acaattctct ttagcaagcg aagcgagcga atcaaacgga gaagcagaag cccattttat
     5581 tcatttgaaa aatacaaaga tttactgtgg agacagtaaa tctactcctt ctaaaggcaa
     5641 aatcttttgg agagggaaaa tagcagaagt agacgggttt ttcttaggaa agatttctga
     5701 tgcaaaatca acgagtaaaa agagttcata aaaaacggcg gggtgattgc cccgccgttt
     5761 tttagtgatg tgatgagatg tgcagcttct tttttcgata catgagcgga gtcatcttca
     5821 tttcttttcg aaactgatta ataaaatagc ttgtactatt aaaccctact tgataggcga
     5881 cttccgtcac atttgcttct gcttgctgca gtccggattc gcgaacgcgt ctcgagagat
     5941 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     6001 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     6061 actatatccg actagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
     6121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa
     6301 aagacgcaga ggaccaattg ggggcacgag tgggatacat agaactggac ttgaatagcg
     6361 gtaaaatcct tgagagtttt cgccctgaag agcgttttcc aatgatgagc actttcaaag
     6421 ttctgctatg tggagcagta ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac
     6481 gaatacacta ttcgcagaat gacttggttg aatactcccc agtgacagaa aagcacctta
     6541 cggacggaat gacggtaaga gaattatgta gtgccgccat aacgatgagt gataacactg
     6601 cggcgaactt acttctgaca accatcggtg gaccgaagga attaaccgct tttttgcaca
     6661 atatgggaga ccatgtaact cgccttgacc gttgggaacc agaactgaat gaagccatac
     6721 caaacgacga gcgagacacc acaatgcctg cggcaatggc aacaacatta cgcaaactat
     6781 taactggcga actacttact ctggcttcac ggcaacaatt aatagactgg cttgaagcgg
     6841 ataaagttgc aggaccacta ctgcgttcgg cacttcctgc tggctggttt attgctgata
     6901 aatctggggc aggagagcgt ggttcacggg gtatcattgc cgcacttgga ccagatggta
     6961 agccttcccg tatcgtagtt atctacacga cgggtagtca ggcaactatg gacgaacgaa
     7021 atagacagat tgctgaaata ggggcttcac tgattaagca ttggtaaacc gatacaatta
     7081 aaggctcctt ttggagcctt tttttttgga cggaccggta gaaaagatca aaggatcttc
     7141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7261 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa
//
