LOCUS       sequence-445978-        7187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpF and GvpL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaFL
FEATURES             Location/Qualifiers
     source          1..7187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5445..5463)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5459..5506
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5573..5664
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6529..6568
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6599..7187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7088..7107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaagtagaa gagaaataac
     2701 gtgcttcaca aattagtaac cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta
     2761 caggaagaag ctgataaaca gctatatgac cttccgacga ttcagcaaaa gctcattcag
     2821 cttcaaatga tgtttgagct tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat
     2881 gaattgttaa tgaggtacga aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta
     2941 acacaaaaaa gaaatgagga atcctagagt gaggaattaa cattatgtct cttaaacaat
     3001 ccatggagaa taaagatatt gctcttattg atattttaga tgtcatttta gataaaggag
     3061 tcgccattaa aggagactta atcatttcca tagctggcgt cgatttagtg tatttggatt
     3121 tgcgggtgct tatttcttcg gttgaaacgc ttgtgcaagc aaaagaagga aatcacaaac
     3181 caatcacttc tgaacaattt gataaacaaa aggaggaatt aatggatgca accggtcagc
     3241 caagcaaatg gacgaatcca cttggatcct gatcaagctg aacaaggctt agcgcagctt
     3301 gtgatgacag ttattgagct attgaggcaa atcgttgaac gtcatgccat gaggcgggtg
     3361 gagggtggaa cgttgacgga cgaacaaatt gaaaacttag gaattgcact aatgaactta
     3421 gaagaaaaaa tggacgagtt gaaagaggtg ttcggtctgg atgcagaaga tttaaatatt
     3481 gatcttggac cgctaggcag cctgctttaa gcggtcagta ggaggaacag tatggcagtc
     3541 gaacataata tgcagtcaag tacgattgta gatgtgctcg aaaagatttt ggataaagga
     3601 gtcgttatag cgggggacat caccgtagga attgcagatg tcgagctatt aacgataaag
     3661 atccgcttga ttgtggcttc ggttgataag gcaaaagaaa tcggcatgga ctggtgggaa
     3721 aatgatccgt atctcagttc aaaaggagcc aataacaaag cgctcgaaga agaaaataaa
     3781 atgctgcatg agcggttaaa aacgcttgaa gaaaaaatag aaacgaaacg ttaaaaactg
     3841 tacgctactt aaaaaatgga gggatttaca atggcaactg aaacaaaatt agataacaca
     3901 caggcagaaa acaaggaaaa taaaaatgcg gaaaacggtt caaaagaaaa gaacggttca
     3961 aaagcaagca aaacaacaag cagcgggcca atcaaacgag cggtagcagg aggcatcatc
     4021 ggtgcaacga ttggatatgt atcgactcct gaaaatcgaa aaagtctcct tgaccgcatt
     4081 gatacagacg aattaaaaag caaagcatct gatttaggaa caaaggtaaa agaaaaatca
     4141 aaaagcagcg tggccagcct gaaaacatct gcgggaagct tgtttaaaaa agataaagat
     4201 aaatcaaaag atgatgaaga aaacgtaaat tcttctagta gcgaaacaga agacgataac
     4261 gttcaagagt acgacgagtt aaaagaagaa aatcaaactc ttcaagatcg cttatcacag
     4321 cttgaagaaa aaatgaacat gcttgttgag cttagcctca ataaaaatca agacgaagaa
     4381 gcggaagata cagattccga cgaagaagag aacgatgaga acgatgaaaa cgatgaaaac
     4441 gagcaggacg atgaaaacga agaagaaaca tctaagccac gtaaaaagga taaaaaagaa
     4501 gctgaggaag aagaaagtga aagtgacgaa gacagcgagg aagaagagga agattctcgc
     4561 tcaaacaaaa aaaataaaaa agtaaaaaca gaagaagaag acgaagatga aagcgaagaa
     4621 gaaaaaaagg aagcgaaacc aaaaaagtca acagctaaaa aatcaaaaaa tacaaaagca
     4681 aagaaaaaca cggacgaaga agatgatgaa gcaacatctc tttctagtga agacgataca
     4741 acagcctaag acgtaaagga ggaaagaaag acatgagtac aggcccttct ttttcaacta
     4801 aagacaatac gcttgaatac tttgtgaaag cttctaataa acacggcttt tcacttgata
     4861 tttcattaaa tgtaaacggc gctgtgattt ccggtaccat gatttcagca aaagaatact
     4921 ttgattactt aagcgaaacg tttgaagaag gcagtgaagt ggctcaggcg ctaagcgaac
     4981 aattctcttt agcaagcgaa gcgagcgaat caaacggaga agcagaagcc cattttattc
     5041 atttgaaaaa tacaaagatt tactgtggag acagtaaatc tactccttct aaaggcaaaa
     5101 tcttttggag agggaaaata gcagaagtag acgggttttt cttaggaaag atttctgatg
     5161 caaaatcaac gagtaaaaag agttcataaa aaacggcggg gtgattgccc cgccgttttt
     5221 tagtgatgtg atgagatgtg cagcttcttt tttcgataca tgagcggagt catcttcatt
     5281 tcttttcgaa actgattaat aaaatagctt gtactattaa accctacttg ataggcgact
     5341 tccgtcacat ttgcttctgc ttgctgcagt ccggattcgc gaacgcgtct cgagagatcc
     5401 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     5461 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     5521 tatatccgac tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta
     5581 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5641 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5701 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctcgt gaaagtaaaa
     5761 gacgcagagg accaattggg ggcacgagtg ggatacatag aactggactt gaatagcggt
     5821 aaaatccttg agagttttcg ccctgaagag cgttttccaa tgatgagcac tttcaaagtt
     5881 ctgctatgtg gagcagtatt atcccgtgta gatgcggggc aagagcaact cggacgacga
     5941 atacactatt cgcagaatga cttggttgaa tactccccag tgacagaaaa gcaccttacg
     6001 gacggaatga cggtaagaga attatgtagt gccgccataa cgatgagtga taacactgcg
     6061 gcgaacttac ttctgacaac catcggtgga ccgaaggaat taaccgcttt tttgcacaat
     6121 atgggagacc atgtaactcg ccttgaccgt tgggaaccag aactgaatga agccatacca
     6181 aacgacgagc gagacaccac aatgcctgcg gcaatggcaa caacattacg caaactatta
     6241 actggcgaac tacttactct ggcttcacgg caacaattaa tagactggct tgaagcggat
     6301 aaagttgcag gaccactact gcgttcggca cttcctgctg gctggtttat tgctgataaa
     6361 tctggggcag gagagcgtgg ttcacggggt atcattgccg cacttggacc agatggtaag
     6421 ccttcccgta tcgtagttat ctacacgacg ggtagtcagg caactatgga cgaacgaaat
     6481 agacagattg ctgaaatagg ggcttcactg attaagcatt ggtaaaccga tacaattaaa
     6541 ggctcctttt ggagcctttt tttttggacg gaccggtaga aaagatcaaa ggatcttctt
     6601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6721 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaa
//
