LOCUS       sequence-445980-        7776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpF and GvpS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaFS
FEATURES             Location/Qualifiers
     source          1..7776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6034..6052)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6048..6095
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6162..6253
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7118..7157
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7188..7776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7677..7696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaagtagaa gagaaataac
     2701 gtgcttcaca aattagtaac cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta
     2761 caggaagaag ctgataaaca gctatatgac cttccgacga ttcagcaaaa gctcattcag
     2821 cttcaaatga tgtttgagct tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat
     2881 gaattgttaa tgaggtacga aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta
     2941 acacaaaaaa gaaatgagga atcctagatg ggagaattac tgtatttata cggtttaatt
     3001 ccaacaaaag aagcagcagc catagagccg tttccatctt ataaggggtt tgacggagaa
     3061 cattcactgt acccaattgc gtttgatcag gtgacggctg tagtttctaa gctggatgct
     3121 gacacctatt cagaaaaagt gattcaagaa aaaatggagc aggatatgag ctggctgcag
     3181 gaaaaagcat ttcatcatca cgaaacggta gccgctttgt acgaagaatt tacgatcatt
     3241 ccattaaaat tttgcaccat ttataaaggt gaagaaagtc tgcaggcagc tattgagatt
     3301 aacaaagaaa agatagagaa ttcactgacg ctgcttcaag gaaatgaaga gtggaatgtg
     3361 aaaatttact gtgatgatac agagcttaaa aaaggaatca gcgaaacgaa tgaaagcgtg
     3421 aaagcgaaaa aacaagaaat tagtcactta tcaccaggaa gacagttttt tgaaaagaaa
     3481 aaaatagatc agctgattga aaaagaatta gagcttcaca aaaacaaagt gtgtgaagag
     3541 atacatgaca agctaaaaga attatcgctt tatgactctg ttaaaaagaa ttggagcaaa
     3601 gacgtaactg gcgcagctga acagatggcg tggaacagcg tgtttcttct cccgtctctg
     3661 cagattacta agttcgtaaa cgaaatagaa gagcttcagc aaaggcttga aaataaaggc
     3721 tggaagtttg aagtgacggg accatggccg ccctatcatt tctcgagctt tgcgtaaaag
     3781 cggttaagcg tgacagtcca ggaggaatta atggatgcaa ccggtcagcc aagcaaatgg
     3841 acgaatccac ttggatcctg atcaagctga acaaggctta gcgcagcttg tgatgacagt
     3901 tattgagcta ttgaggcaaa tcgttgaacg tcatgccatg aggcgggtgg agggtggaac
     3961 gttgacggac gaacaaattg aaaacttagg aattgcacta atgaacttag aagaaaaaat
     4021 ggacgagttg aaagaggtgt tcggtctgga tgcagaagat ttaaatattg atcttggacc
     4081 gctaggcagc ctgctttaag cggtcagtag gaggaacagt atggcagtcg aacataatat
     4141 gcagtcaagt acgattgtag atgtgctcga aaagattttg gataaaggag tcgttatagc
     4201 gggggacatc accgtaggaa ttgcagatgt cgagctatta acgataaaga tccgcttgat
     4261 tgtggcttcg gttgataagg caaaagaaat cggcatggac tggtgggaaa atgatccgta
     4321 tctcagttca aaaggagcca ataacaaagc gctcgaagaa gaaaataaaa tgctgcatga
     4381 gcggttaaaa acgcttgaag aaaaaataga aacgaaacgt taaaaactgt acgctactta
     4441 aaaaatggag ggatttacaa tggcaactga aacaaaatta gataacacac aggcagaaaa
     4501 caaggaaaat aaaaatgcgg aaaacggttc aaaagaaaag aacggttcaa aagcaagcaa
     4561 aacaacaagc agcgggccaa tcaaacgagc ggtagcagga ggcatcatcg gtgcaacgat
     4621 tggatatgta tcgactcctg aaaatcgaaa aagtctcctt gaccgcattg atacagacga
     4681 attaaaaagc aaagcatctg atttaggaac aaaggtaaaa gaaaaatcaa aaagcagcgt
     4741 ggccagcctg aaaacatctg cgggaagctt gtttaaaaaa gataaagata aatcaaaaga
     4801 tgatgaagaa aacgtaaatt cttctagtag cgaaacagaa gacgataacg ttcaagagta
     4861 cgacgagtta aaagaagaaa atcaaactct tcaagatcgc ttatcacagc ttgaagaaaa
     4921 aatgaacatg cttgttgagc ttagcctcaa taaaaatcaa gacgaagaag cggaagatac
     4981 agattccgac gaagaagaga acgatgagaa cgatgaaaac gatgaaaacg agcaggacga
     5041 tgaaaacgaa gaagaaacat ctaagccacg taaaaaggat aaaaaagaag ctgaggaaga
     5101 agaaagtgaa agtgacgaag acagcgagga agaagaggaa gattctcgct caaacaaaaa
     5161 aaataaaaaa gtaaaaacag aagaagaaga cgaagatgaa agcgaagaag aaaaaaagga
     5221 agcgaaacca aaaaagtcaa cagctaaaaa atcaaaaaat acaaaagcaa agaaaaacac
     5281 ggacgaagaa gatgatgaag caacatctct ttctagtgaa gacgatacaa cagcctaaga
     5341 cgtaaaggag gaaagaaaga catgagtaca ggcccttctt tttcaactaa agacaatacg
     5401 cttgaatact ttgtgaaagc ttctaataaa cacggctttt cacttgatat ttcattaaat
     5461 gtaaacggcg ctgtgatttc cggtaccatg atttcagcaa aagaatactt tgattactta
     5521 agcgaaacgt ttgaagaagg cagtgaagtg gctcaggcgc taagcgaaca attctcttta
     5581 gcaagcgaag cgagcgaatc aaacggagaa gcagaagccc attttattca tttgaaaaat
     5641 acaaagattt actgtggaga cagtaaatct actccttcta aaggcaaaat cttttggaga
     5701 gggaaaatag cagaagtaga cgggtttttc ttaggaaaga tttctgatgc aaaatcaacg
     5761 agtaaaaaga gttcataaaa aacggcgggg tgattgcccc gccgtttttt agtgatgtga
     5821 tgagatgtgc agcttctttt ttcgatacat gagcggagtc atcttcattt cttttcgaaa
     5881 ctgattaata aaatagcttg tactattaaa ccctacttga taggcgactt ccgtcacatt
     5941 tgcttctgct tgctgcagtc cggattcgcg aacgcgtctc gagagatccg gctgctaaca
     6001 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     6061 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccgact
     6121 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
     6181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6241 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6301 attttgcctt cctgtttttg ctcacccaga aacgctcgtg aaagtaaaag acgcagagga
     6361 ccaattgggg gcacgagtgg gatacataga actggacttg aatagcggta aaatccttga
     6421 gagttttcgc cctgaagagc gttttccaat gatgagcact ttcaaagttc tgctatgtgg
     6481 agcagtatta tcccgtgtag atgcggggca agagcaactc ggacgacgaa tacactattc
     6541 gcagaatgac ttggttgaat actccccagt gacagaaaag caccttacgg acggaatgac
     6601 ggtaagagaa ttatgtagtg ccgccataac gatgagtgat aacactgcgg cgaacttact
     6661 tctgacaacc atcggtggac cgaaggaatt aaccgctttt ttgcacaata tgggagacca
     6721 tgtaactcgc cttgaccgtt gggaaccaga actgaatgaa gccataccaa acgacgagcg
     6781 agacaccaca atgcctgcgg caatggcaac aacattacgc aaactattaa ctggcgaact
     6841 acttactctg gcttcacggc aacaattaat agactggctt gaagcggata aagttgcagg
     6901 accactactg cgttcggcac ttcctgctgg ctggtttatt gctgataaat ctggggcagg
     6961 agagcgtggt tcacggggta tcattgccgc acttggacca gatggtaagc cttcccgtat
     7021 cgtagttatc tacacgacgg gtagtcaggc aactatggac gaacgaaata gacagattgc
     7081 tgaaataggg gcttcactga ttaagcattg gtaaaccgat acaattaaag gctccttttg
     7141 gagccttttt ttttggacgg accggtagaa aagatcaaag gatcttcttg agatcctttt
     7201 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7261 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7321 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7381 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7441 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7501 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7561 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7621 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7681 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7741 ttgtgatgct cgtcaggggg gcggagccta tggaaa
//
