LOCUS       sequence-44599-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Eguchi A, Meade BR, Chang YC, Fredrickson CT, Willert K, Puri N, 
            Dowdy SF
  TITLE     Efficient siRNA delivery into primary cells by a peptide 
            transduction domain-dsRNA binding domain fusion protein.
  JOURNAL   Nat Biotechnol. 2009 Jun;27(6):567-71. Epub 2009 May 17.
  PUBMED    19448630
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2009-06"; volume: "27(6)"; pages: "567-71. Epub
            2009 May 17"
COMMENT     Sequence Label: pPTD-DRBD
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..138
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             439..456
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(509..527)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      523..570
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(694..713)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagga agaagcggag acagcgacga
       61 agaggccata gcggccgtaa aaagcgtcgt cagcgtcgtc gtggccatat ttatccgtac
      121 gatgtgccgg actatgcggg ggatccgggt cgtaagaagc gccgtcagcg ccgtcgcggg
      181 gatccggctg gtgatctttc agcaggtttc ttcatggagg aacttaatac ataccgtcag
      241 aagcagggag tagtacttaa atatcaagaa ctgcctaatt caggacctcc acatgatagg
      301 aggtttacat ttcaagttat aatagatgga agagaatttc cagaaggtga aggtagatca
      361 aagaaggaag caaaaaatgc cgcagccaaa ttagctgttg agatacttaa taaggaaaag
      421 aaggcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      481 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      541 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      601 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      661 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      721 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      781 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
//
