LOCUS       sequence-44600-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Eguchi A, Meade BR, Chang YC, Fredrickson CT, Willert K, Puri N, 
            Dowdy SF
  TITLE     Efficient siRNA delivery into primary cells by a peptide 
            transduction domain-dsRNA binding domain fusion protein.
  JOURNAL   Nat Biotechnol. 2009 Jun;27(6):567-71. Epub 2009 May 17.
  PUBMED    19448630
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2009-06"; volume: "27(6)"; pages: "567-71. Epub
            2009 May 17"
COMMENT     Sequence Label: pPTD-DRBD
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(335..361)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             449..471
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    486..510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(510..529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(511..529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(613..632)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgcc ttcttttcct
       61 tattaagtat ctcaacagct aatttggctg cggcattttt tgcttccttc tttgatctac
      121 cttcaccttc tggaaattct cttccatcta ttataacttg aaatgtaaac ctcctatcat
      181 gtggaggtcc tgaattaggc agttcttgat atttaagtac tactccctgc ttctgacggt
      241 atgtattaag ttcctccatg aagaaacctg ctgaaagatc accagccgga tccccgcgac
      301 ggcgctgacg gcgcttctta cgacccggat cccccgcata gtccggcaca tcgtacggat
      361 aaatatggcc acgacgacgc tgacgacgct ttttacggcc gctatggcct cttcgtcgct
      421 gtctccgctt cttcctgccc atggtatatc tccttcttaa agttaaacaa aattatttct
      481 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga
      541 tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      601 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
      661 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
      721 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
      781 tactactggg ctgcttccta atgcaggagt cgca
//
