LOCUS       sequence-446024-        7860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaT
FEATURES             Location/Qualifiers
     source          1..7860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..129
                     /gene="bla"
                     /label=AmpR promoter
     terminator      994..1033
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1064..1652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1553..1572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1806..1823
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1838..1977
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1903..1922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2063..2085
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2079..2270)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2507..2526
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2507..2525
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4914..4931
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(7770..7788)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7784..7831
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt ctaaatacat
       61 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      121 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      181 tgccttcctg tttttgctca cccagaaacg ctcgtgaaag taaaagacgc agaggaccaa
      241 ttgggggcac gagtgggata catagaactg gacttgaata gcggtaaaat ccttgagagt
      301 tttcgccctg aagagcgttt tccaatgatg agcactttca aagttctgct atgtggagca
      361 gtattatccc gtgtagatgc ggggcaagag caactcggac gacgaataca ctattcgcag
      421 aatgacttgg ttgaatactc cccagtgaca gaaaagcacc ttacggacgg aatgacggta
      481 agagaattat gtagtgccgc cataacgatg agtgataaca ctgcggcgaa cttacttctg
      541 acaaccatcg gtggaccgaa ggaattaacc gcttttttgc acaatatggg agaccatgta
      601 actcgccttg accgttggga accagaactg aatgaagcca taccaaacga cgagcgagac
      661 accacaatgc ctgcggcaat ggcaacaaca ttacgcaaac tattaactgg cgaactactt
      721 actctggctt cacggcaaca attaatagac tggcttgaag cggataaagt tgcaggacca
      781 ctactgcgtt cggcacttcc tgctggctgg tttattgctg ataaatctgg ggcaggagag
      841 cgtggttcac ggggtatcat tgccgcactt ggaccagatg gtaagccttc ccgtatcgta
      901 gttatctaca cgacgggtag tcaggcaact atggacgaac gaaatagaca gattgctgaa
      961 ataggggctt cactgattaa gcattggtaa accgatacaa ttaaaggctc cttttggagc
     1021 cttttttttt ggacggaccg gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1081 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1141 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1201 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1261 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1321 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1381 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1441 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1501 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1561 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1621 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1681 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1741 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1801 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     1861 cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc
     1921 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     1981 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     2041 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     2101 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     2161 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     2221 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     2281 gtgtaagggg gatttctgtt catggggtaa tgataccgat gaaacgagag aggatgctca
     2341 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2401 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2461 ttaatacaga tgtaggtgtt ggcaccatgc atcgatcccg cgaaattaat acgactcact
     2521 atagggagac cacaacggtt tccctctaga gatagcttag cgatgtgaaa gaagctggag
     2581 aaaactttca agaaaaagtt tcttcttcag acgataaaga gaagtcaaaa aataagcgga
     2641 aaataaaagg cgtaaatcag attaaaaaag caacggatat taaaagctct actaagatta
     2701 aaagttctaa tgatattaaa tcatctacag atttaaaaac aatgggatca taagcgaaag
     2761 gagaaatgag atatgtctat tcaaaaaagt actaatagtt caagtttagc agaagtcatt
     2821 gaccgtattt tagataaagg aattgttatt gatgcttttg caagagtttc tgttgtagga
     2881 attgaaattt taacgattga agcgcgagtg gttattgcca gtgttgatac atggttacgc
     2941 tatgcagaag cagtagggct tcttcgtgac gacgtagaag aaaacggtct tcctgaacgt
     3001 tcaaattcaa gtgaagggca gccgcgtttt agtatttaat atgaagtggt atggaacctt
     3061 ccttttttga gggtggttcc agcttttaga tctaactatt ggaggctact aaaaatggaa
     3121 attaaaaaaa ttatgcaagc cgtgaacgac tttttcggtg aacacgtagc tcctcctcat
     3181 aaaattacct cggtggaagc tactgaagat gaaggttgga gagttattgt tgaagtcatt
     3241 gaagaacgag aatatatgaa aaaatacgcc aaagatgaaa tgctcggaac gtacgagtgc
     3301 tttgtaaata aagaaaaaga agtcatttca ttcaaacgac tcgacgtcag atatagaagc
     3361 gccattggca ttgaagcata aacgataaga tggcaggagg aacgtaaaaa tgaccgtctt
     3421 aacagacaaa aggaaaaaag gcagtggagc ttttatacaa gatgacgaga caaaagaggt
     3481 tctttcaaga gcgctgagct atttaaaatc cggctattcc attcatttta caggtcctgc
     3541 cggcggaggc aaaacctctt tagcgcgagc gcttgctaaa aagagaaagc gtcctgtaat
     3601 gctgatgcac gggaatcacg agctcaacaa caaagattta attggcgatt ttacgggata
     3661 cacgagcaaa aaagtaatcg accagtacgt tcgttctgtc tataaaaaag atgaacaggt
     3721 gagtgaaaac tggcaggatg gccgattgct tgaagctgta aaaaatggct atacgctgat
     3781 ttacgacgaa tttactcgtt ctaagcctgc gacgaataat atctttctat cgatattaga
     3841 agaaggcgtg ctgccgctgt atggagtaaa aatgaccgat ccttttgtgc gcgtgcatcc
     3901 cgatttccgc gtcatcttca caagcaatcc agctgagtat gccggcgtat atgatacgca
     3961 agatgcgctt ctcgacaggt taattaccat gtttattgat tataaagaca tcgacagaga
     4021 gacagcgatt ttaacggaga aaacggacgt agaagaagat gaagcgcgca caattgtaac
     4081 gctcgtagca aacgtgcgaa accgctctgg agacgaaaac agcagcggac ttagcctgcg
     4141 ggcttcgctt atgatcgcta cccttgccac gcagcaagac attcctatcg atggaagtga
     4201 cgaagatttt caaacgttat gtatcgatat tttgcatcat ccgcttacca aatgtttgga
     4261 tgaagaaaat gcaaaaagca aagccgaaaa aatcatttta gaagaatgta agaatataga
     4321 cactgaagaa aagtaaagga gcttgaaaac atgagtgaaa caaacgaaac aggtatttat
     4381 atttttagcg ccattcaaac ggataaagac gaagaatttg gcgccgtgga agtagaagga
     4441 acaaaagctg aaacattttt gattcgctac aaagacgcgg ctatggtagc agctgaagta
     4501 ccgatgaaaa tttatcatcc taatcgccaa aatttattaa tgcatcaaaa cgcagtagca
     4561 gcgattatgg acaagaacga tacggttatt ccaatcagct ttgggaatgt attcaaatca
     4621 aaagaagacg taaaagttct tttggaaaac ctttatccgc agtttgaaaa gctgtttcca
     4681 gcgatcaaag gaaaaattga agtcggttta aaagtaattg ggaaaaaaga atggcttgag
     4741 aaaaaagtaa acgaaaatcc tgaacttgag aaagtatcag catccgtaaa aggaaaatca
     4801 gaagcagccg gttattatga gcgtattcaa cttggaggaa tggctcaaaa gatgtttact
     4861 tccctgcaaa aagaagtcaa gacagatgtg ttttctccgc ttgaagaagc agcggaagca
     4921 gcaaaagcaa atgagccaac gggcgaaacg atgcttttaa acgcgtcttt cttaattaac
     4981 cgagaagatg aagcgaagtt tgatgaaaaa gtaaatgaag cgcatgaaaa ctggaaagac
     5041 aaagccgatt ttcattacag cggtccttgg cctgcttata attttgtgaa cattcgccta
     5101 aaagtagaag agaaataacg tgcttcacaa attagtaacc gcacccatta accttgtagt
     5161 gaaaatcggc gaaaaagtac aggaagaagc tgataaacag ctatatgacc ttccgacgat
     5221 tcagcaaaag ctcattcagc ttcaaatgat gtttgagctt ggtgaaattc cagaagaagc
     5281 gtttcaagaa aaagaagatg aattgttaat gaggtacgaa attgcgaaac gcagagaaat
     5341 tgaacaatgg gaagagctaa cacaaaaaag aaatgaggaa tcctagatgg gagaattact
     5401 gtatttatac ggtttaattc caacaaaaga agcagcagcc atagagccgt ttccatctta
     5461 taaggggttt gacggagaac attcactgta cccaattgcg tttgatcagg tgacggctgt
     5521 agtttctaag ctggatgctg acacctattc agaaaaagtg attcaagaaa aaatggagca
     5581 ggatatgagc tggctgcagg aaaaagcatt tcatcatcac gaaacggtag ccgctttgta
     5641 cgaagaattt acgatcattc cattaaaatt ttgcaccatt tataaaggtg aagaaagtct
     5701 gcaggcagct attgagatta acaaagaaaa gatagagaat tcactgacgc tgcttcaagg
     5761 aaatgaagag tggaatgtga aaatttactg tgatgataca gagcttaaaa aaggaatcag
     5821 cgaaacgaat gaaagcgtga aagcgaaaaa acaagaaatt agtcacttat caccaggaag
     5881 acagtttttt gaaaagaaaa aaatagatca gctgattgaa aaagaattag agcttcacaa
     5941 aaacaaagtg tgtgaagaga tacatgacaa gctaaaagaa ttatcgcttt atgactctgt
     6001 taaaaagaat tggagcaaag acgtaactgg cgcagctgaa cagatggcgt ggaacagcgt
     6061 gtttcttctc ccgtctctgc agattactaa gttcgtaaac gaaatagaag agcttcagca
     6121 aaggcttgaa aataaaggct ggaagtttga agtgacggga ccatggccgc cctatcattt
     6181 ctcgagcttt gcgtaaagtg aggaattaac attatgtctc ttaaacaatc catggagaat
     6241 aaagatattg ctcttattga tattttagat gtcattttag ataaaggagt cgccattaaa
     6301 ggagacttaa tcatttccat agctggcgtc gatttagtgt atttggattt gcgggtgctt
     6361 atttcttcgg ttgaaacgct tgtgcaagca aaagaaggaa atcacaaacc aatcacttct
     6421 gaacaatttg ataaacaaaa ggaggaatta atggatgcaa ccggtcagcc aagcaaatgg
     6481 acgaatccac ttggatcctg atcaagctga acaaggctta gcgcagcttg tgatgacagt
     6541 tattgagcta ttgaggcaaa tcgttgaacg tcatgccatg aggcgggtgg agggtggaac
     6601 gttgacggac gaacaaattg aaaacttagg aattgcacta atgaacttag aagaaaaaat
     6661 ggacgagttg aaagaggtgt tcggtctgga tgcagaagat ttaaatattg atcttggacc
     6721 gctaggcagc ctgctttaag cggtcagtag gaggaacagt atggcagtcg aacataatat
     6781 gcagtcaagt acgattgtag atgtgctcga aaagattttg gataaaggag tcgttatagc
     6841 gggggacatc accgtaggaa ttgcagatgt cgagctatta acgataaaga tccgcttgat
     6901 tgtggcttcg gttgataagg caaaagaaat cggcatggac tggtgggaaa atgatccgta
     6961 tctcagttca aaaggagcca ataacaaagc gctcgaagaa gaaaataaaa tgctgcatga
     7021 gcggttaaaa acgcttgaag aaaaaataga aacgaaacgt taaaaactgt acgctactta
     7081 aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac aatacgcttg
     7141 aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca ttaaatgtaa
     7201 acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat tacttaagcg
     7261 aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc tctttagcaa
     7321 gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg aaaaatacaa
     7381 agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt tggagaggga
     7441 aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa tcaacgagta
     7501 aaaagagttc ataaaaaacg gcggggtgat tgccccgccg ttttttagtg atgtgatgag
     7561 atgtgcagct tcttttttcg atacatgagc ggagtcatct tcatttcttt tcgaaactga
     7621 ttaataaaat agcttgtact attaaaccct acttgatagg cgacttccgt cacatttgct
     7681 tctgcttgct gcagtccgga ttcgcgaacg cgtctcgaga gatccggctg ctaacaaagc
     7741 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     7801 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccgactagta
//
