LOCUS       sequence-446026-        8179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpB and GvpG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaBG
FEATURES             Location/Qualifiers
     source          1..8179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2927..2944
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6437..6455)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6451..6498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6565..6656
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7521..7560
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7591..8179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8080..8099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcatt agatctaact attggaggct actaaaaatg gaaattaaaa
     1141 aaattatgca agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta
     1201 cctcggtgga agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac
     1261 gagaatatat gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa
     1321 ataaagaaaa agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg
     1381 gcattgaagc ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac
     1441 aaaaggaaaa aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca
     1501 agagcgctga gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga
     1561 ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg
     1621 cacgggaatc acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc
     1681 aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa
     1741 aactggcagg atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac
     1801 gaatttactc gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc
     1861 gtgctgccgc tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc
     1921 cgcgtcatct tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg
     1981 cttctcgaca ggttaattac catgtttatt gattataaag acatcgacag agagacagcg
     2041 attttaacgg agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta
     2101 gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg
     2161 cttatgatcg ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat
     2221 tttcaaacgt tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa
     2281 aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa
     2341 gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga aacaggtatt tatattttta
     2401 gcgccattca aacggataaa gacgaagaat ttggcgccgt ggaagtagaa ggaacaaaag
     2461 ctgaaacatt tttgattcgc tacaaagacg cggctatggt agcagctgaa gtaccgatga
     2521 aaatttatca tcctaatcgc caaaatttat taatgcatca aaacgcagta gcagcgatta
     2581 tggacaagaa cgatacggtt attccaatca gctttgggaa tgtattcaaa tcaaaagaag
     2641 acgtaaaagt tcttttggaa aacctttatc cgcagtttga aaagctgttt ccagcgatca
     2701 aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa agaatggctt gagaaaaaag
     2761 taaacgaaaa tcctgaactt gagaaagtat cagcatccgt aaaaggaaaa tcagaagcag
     2821 ccggttatta tgagcgtatt caacttggag gaatggctca aaagatgttt acttccctgc
     2881 aaaaagaagt caagacagat ttgttttctc cgcttgaaga agcagcggaa gcagcaaaag
     2941 caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc tttcttaatt aaccgagaag
     3001 atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga aaactggaaa gacaaagccg
     3061 attttcatta cagcggtcct tggcctgctt ataattttgt gaacattcgc ctaaaagtag
     3121 aagagaaata acgaaatgag gaatcctaga tgggagaatt actgtattta tacggtttaa
     3181 ttccaacaaa agaagcagca gccatagagc cgtttccatc ttataagggg tttgacggag
     3241 aacattcact gtacccaatt gcgtttgatc aggtgacggc tgtagtttct aagctggatg
     3301 ctgacaccta ttcagaaaaa gtgattcaag aaaaaatgga gcaggatatg agctggctgc
     3361 aggaaaaagc atttcatcat cacgaaacgg tagccgcttt gtacgaagaa tttacgatca
     3421 ttccattaaa attttgcacc atttataaag gtgaagaaag tctgcaggca gctattgaga
     3481 ttaacaaaga aaagatagag aattcactga cgctgcttca aggaaatgaa gagtggaatg
     3541 tgaaaattta ctgtgatgat acagagctta aaaaaggaat cagcgaaacg aatgaaagcg
     3601 tgaaagcgaa aaaacaagaa attagtcact tatcactagg aagacagttt tttgaaaaga
     3661 aaaaaataga tcagctgatt gaaaaagaat tagagcttca caaaaacaaa gtgtgtgaag
     3721 agatacatga caagctaaaa gaattatcgc tttatgactc tgttaaaaag aattggagca
     3781 aagacgtaac tggcgcagct gaacagatgg cgtggaacag cgtgtttctt ctcccgtctc
     3841 tgcagattac taagttcgta aacgaaatag aagagcttca gcaaaggctt gaaaataaag
     3901 gctggaagtt tgaagtgacg ggaccatggc cgccctatca tttctcgagc tttgcgtaaa
     3961 gtgaggaatt aacattatgt ctcttaaaca atccatggag aataaagata ttgctcttat
     4021 tgatatttta gatgtcattt tagataaagg agtcgccatt aaaggagact taatcatttc
     4081 catagctggc gtcgatttag tgtatttgga tttgcgggtg cttatttctt cggttgaaac
     4141 gcttgtgcaa gcaaaagaag gaaatcacaa accaatcact tctgaacaat ttgataaaca
     4201 aaaggaggaa ttaatggatg caaccggtca gccaagcaaa tggacgaatc cacttggatc
     4261 ctgatcaagc tgaacaaggc ttagcgcagc ttgtgatgac agttattgag ctattgaggc
     4321 aaatcgttga acgtcatgcc atgaggcggg tggagggtgg aacgttgacg gacgaacaaa
     4381 ttgaaaactt aggaattgca ctaatgaact tagaagaaaa aatggacgag ttgaaagagg
     4441 tgttcggtct ggatgcagaa gatttaaata ttgatcttgg accgctaggc agcctgcttt
     4501 aagcggtcag taggaggaac agtatggcag tcgaacataa tatgcagtca agtacgattg
     4561 tagatgtgct cgaaaagatt ttggataaag gagtcgttat agcgggggac atcaccgtag
     4621 gaattgcaga tgtcgagcta ttaacgataa agatccgctt gattgtggct tcggttgata
     4681 aggcaaaaga aatcggcatg gactggtggg aaaatgatcc gtatctcagt tcaaaaggag
     4741 ccaataacaa agcgctcgaa gaagaaaata aaatgctgca tgagcggtta aaaacgcttg
     4801 aagaaaaaat agaaacgaaa cgttaaaaac tgtacgctac ttaaaaaatg gagggattta
     4861 caatggcaac tgaaacaaaa ttagataaca cacaggcaga aaacaaggaa aataaaaatg
     4921 cggaaaacgg ttcaaaagaa aagaacggtt caaaagcaag caaaacaaca agcagcgggc
     4981 caatcaaacg agcggtagca ggaggcatca tcggtgcaac gattggatat gtatcgactc
     5041 ctgaaaatcg aaaaagtctc cttgaccgca ttgatacaga cgaattaaaa agcaaagcat
     5101 ctgatttagg aacaaaggta aaagaaaaat caaaaagcag cgtggccagc ctgaaaacat
     5161 ctgcgggaag cttgtttaaa aaagataaag ataaatcaaa agatgatgaa gaaaacgtaa
     5221 attcttctag tagcgaaaca gaagacgata acgttcaaga gtacgacgag ttaaaagaag
     5281 aaaatcaaac tcttcaagat cgcttatcac agcttgaaga aaaaatgaac atgcttgttg
     5341 agcttagcct caataaaaat caagacgaag aagcggaaga tacagattcc gacgaagaag
     5401 agaacgatga gaacgatgaa aacgatgaaa acgagcagga cgatgaaaac gaagaagaaa
     5461 catctaagcc acgtaaaaag gataaaaaag aagctgagga agaagaaagt gaaagtgacg
     5521 aagacagcga ggaagaagag gaagattctc gctcaaacaa aaaaaataaa aaagtaaaaa
     5581 cagaagaaga agacgaagat gaaagcgaag aagaaaaaaa ggaagcgaaa ccaaaaaagt
     5641 caacagctaa aaaatcaaaa aatacaaaag caaagaaaaa cacggacgaa gaagatgatg
     5701 aagcaacatc tctttctagt gaagacgata caacagccta agacgtaaag gaggaaagaa
     5761 agacatgagt acaggccctt ctttttcaac taaagacaat acgcttgaat actttgtgaa
     5821 agcttctaat aaacacggct tttcacttga tatttcatta aatgtaaacg gcgctgtgat
     5881 ttccggtacc atgatttcag caaaagaata ctttgattac ttaagcgaaa cgtttgaaga
     5941 aggcagtgaa gtggctcagg cgctaagcga acaattctct ttagcaagcg aagcgagcga
     6001 atcaaacgga gaagcagaag cccattttat tcatttgaaa aatacaaaga tttactgtgg
     6061 agacagtaaa tctactcctt ctaaaggcaa aatcttttgg agagggaaaa tagcagaagt
     6121 agacgggttt ttcttaggaa agatttctga tgcaaaatca acgagtaaaa agagttcata
     6181 aaaaacggcg gggtgattgc cccgccgttt tttagtgatg tgatgagatg tgcagcttct
     6241 tttttcgata catgagcgga gtcatcttca tttcttttcg aaactgatta ataaaatagc
     6301 ttgtactatt aaaccctact tgataggcga cttccgtcac atttgcttct gcttgctgca
     6361 gtccggattc gcgaacgcgt ctcgagagat ccggctgcta acaaagcccg aaaggaagct
     6421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     6481 gtcttgaggg gttttttgct gaaaggagga actatatccg actagtagcc cgcctaatga
     6541 gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc
     6601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6721 ttgctcaccc agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag
     6781 tgggatacat agaactggac ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag
     6841 agcgttttcc aatgatgagc actttcaaag ttctgctatg tggagcagta ttatcccgtg
     6901 tagatgcggg gcaagagcaa ctcggacgac gaatacacta ttcgcagaat gacttggttg
     6961 aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta
     7021 gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg
     7081 gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc
     7141 gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg
     7201 cggcaatggc aacaacatta cgcaaactat taactggcga actacttact ctggcttcac
     7261 ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg
     7321 cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg
     7381 gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga
     7441 cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac
     7501 tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga
     7561 cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8161 ggggcggagc ctatggaaa
//
