LOCUS       sequence-446027-        7663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpF and GvpJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaFJ
FEATURES             Location/Qualifiers
     source          1..7663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5921..5939)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5935..5982
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6049..6140
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7005..7044
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7075..7663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7564..7583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaagtagaa gagaaataac
     2701 gtgcttcaca aattagtaac cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta
     2761 caggaagaag ctgataaaca gctatatgac cttccgacga ttcagcaaaa gctcattcag
     2821 cttcaaatga tgtttgagct tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat
     2881 gaattgttaa tgaggtacga aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta
     2941 acacaaaaaa gaaatgagga atcctagatg ggagaattac tgtatttata cggtttaatt
     3001 ccaacaaaag aagcagcagc catagagccg tttccatctt ataaggggtt tgacggagaa
     3061 cattcactgt acccaattgc gtttgatcag gtgacggctg tagtttctaa gctggatgct
     3121 gacacctatt cagaaaaagt gattcaagaa aaaatggagc aggatatgag ctggctgcag
     3181 gaaaaagcat ttcatcatca cgaaacggta gccgctttgt acgaagaatt tacgatcatt
     3241 ccattaaaat tttgcaccat ttataaaggt gaagaaagtc tgcaggcagc tattgagatt
     3301 aacaaagaaa agatagagaa ttcactgacg ctgcttcaag gaaatgaaga gtggaatgtg
     3361 aaaatttact gtgatgatac agagcttaaa aaaggaatca gcgaaacgaa tgaaagcgtg
     3421 aaagcgaaaa aacaagaaat tagtcactta tcaccaggaa gacagttttt tgaaaagaaa
     3481 aaaatagatc agctgattga aaaagaatta gagcttcaca aaaacaaagt gtgtgaagag
     3541 atacatgaca agctaaaaga attatcgctt tatgactctg ttaaaaagaa ttggagcaaa
     3601 gacgtaactg gcgcagctga acagatggcg tggaacagcg tgtttcttct cccgtctctg
     3661 cagattacta agttcgtaaa cgaaatagaa gagcttcagc aaaggcttga aaataaaggc
     3721 tggaagtttg aagtgacggg accatggccg ccctatcatt tctcgagctt tgcgtaaagt
     3781 gaggaattaa cattatgtct cttaaacaat ccatggagaa taaagatatt gctcttattg
     3841 atattttaga tgtcatttta gataaaggag tcgccattaa aggagactta atcatttcca
     3901 tagctggcgt cgatttagtg tatttggatt tgcgggtgct tatttcttcg gttgaaacgc
     3961 ttgtgcaagc aaaagaagga aatcacaaac caatcacttc tgaacaattt gataaacaaa
     4021 aggaggaatt aatggatgca accggtcagc caagcaaatg gacgaatcca cttggatcct
     4081 gatcaagctg aacaaggctt agcgcagctt gtgatgacag ttattgagct attgaggcaa
     4141 atcgttgaac gtcatgccat gaggcgggtg gagggtggaa cgttgacgga cgaacaaatt
     4201 gaaaacttag gaattgcact aatgaactta gaagaaaaaa tggacgagtt gaaagaggtg
     4261 ttcggtctgg atgcagaaga tttaaatatt gatcttggac cgctaggcag cctgctttaa
     4321 gcggtcaaaa aatggaggga tttacaatgg caactgaaac aaaattagat aacacacagg
     4381 cagaaaacaa ggaaaataaa aatgcggaaa acggttcaaa agaaaagaac ggttcaaaag
     4441 caagcaaaac aacaagcagc gggccaatca aacgagcggt agcaggaggc atcatcggtg
     4501 caacgattgg atatgtatcg actcctgaaa atcgaaaaag tctccttgac cgcattgata
     4561 cagacgaatt aaaaagcaaa gcatctgatt taggaacaaa ggtaaaagaa aaatcaaaaa
     4621 gcagcgtggc cagcctgaaa acatctgcgg gaagcttgtt taaaaaagat aaagataaat
     4681 caaaagatga tgaagaaaac gtaaattctt ctagtagcga aacagaagac gataacgttc
     4741 aagagtacga cgagttaaaa gaagaaaatc aaactcttca agatcgctta tcacagcttg
     4801 aagaaaaaat gaacatgctt gttgagctta gcctcaataa aaatcaagac gaagaagcgg
     4861 aagatacaga ttccgacgaa gaagagaacg atgagaacga tgaaaacgat gaaaacgagc
     4921 aggacgatga aaacgaagaa gaaacatcta agccacgtaa aaaggataaa aaagaagctg
     4981 aggaagaaga aagtgaaagt gacgaagaca gcgaggaaga agaggaagat tctcgctcaa
     5041 acaaaaaaaa taaaaaagta aaaacagaag aagaagacga agatgaaagc gaagaagaaa
     5101 aaaaggaagc gaaaccaaaa aagtcaacag ctaaaaaatc aaaaaataca aaagcaaaga
     5161 aaaacacgga cgaagaagat gatgaagcaa catctctttc tagtgaagac gatacaacag
     5221 cctaagacgt aaaggaggaa agaaagacat gagtacaggc ccttcttttt caactaaaga
     5281 caatacgctt gaatactttg tgaaagcttc taataaacac ggcttttcac ttgatatttc
     5341 attaaatgta aacggcgctg tgatttccgg taccatgatt tcagcaaaag aatactttga
     5401 ttacttaagc gaaacgtttg aagaaggcag tgaagtggct caggcgctaa gcgaacaatt
     5461 ctctttagca agcgaagcga gcgaatcaaa cggagaagca gaagcccatt ttattcattt
     5521 gaaaaataca aagatttact gtggagacag taaatctact ccttctaaag gcaaaatctt
     5581 ttggagaggg aaaatagcag aagtagacgg gtttttctta ggaaagattt ctgatgcaaa
     5641 atcaacgagt aaaaagagtt cataaaaaac ggcggggtga ttgccccgcc gttttttagt
     5701 gatgtgatga gatgtgcagc ttcttttttc gatacatgag cggagtcatc ttcatttctt
     5761 ttcgaaactg attaataaaa tagcttgtac tattaaaccc tacttgatag gcgacttccg
     5821 tcacatttgc ttctgcttgc tgcagtccgg attcgcgaac gcgtctcgag agatccggct
     5881 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     5941 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     6001 tccgactagt agcccgccta atgagcgggc ttttttttaa ttcccctatt tgtttatttt
     6061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg
     6241 cagaggacca attgggggca cgagtgggat acatagaact ggacttgaat agcggtaaaa
     6301 tccttgagag ttttcgccct gaagagcgtt ttccaatgat gagcactttc aaagttctgc
     6361 tatgtggagc agtattatcc cgtgtagatg cggggcaaga gcaactcgga cgacgaatac
     6421 actattcgca gaatgacttg gttgaatact ccccagtgac agaaaagcac cttacggacg
     6481 gaatgacggt aagagaatta tgtagtgccg ccataacgat gagtgataac actgcggcga
     6541 acttacttct gacaaccatc ggtggaccga aggaattaac cgcttttttg cacaatatgg
     6601 gagaccatgt aactcgcctt gaccgttggg aaccagaact gaatgaagcc ataccaaacg
     6661 acgagcgaga caccacaatg cctgcggcaa tggcaacaac attacgcaaa ctattaactg
     6721 gcgaactact tactctggct tcacggcaac aattaataga ctggcttgaa gcggataaag
     6781 ttgcaggacc actactgcgt tcggcacttc ctgctggctg gtttattgct gataaatctg
     6841 gggcaggaga gcgtggttca cggggtatca ttgccgcact tggaccagat ggtaagcctt
     6901 cccgtatcgt agttatctac acgacgggta gtcaggcaac tatggacgaa cgaaatagac
     6961 agattgctga aataggggct tcactgatta agcattggta aaccgataca attaaaggct
     7021 ccttttggag cctttttttt tggacggacc ggtagaaaag atcaaaggat cttcttgaga
     7081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7201 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaa
//
