LOCUS       sequence-446028-        7165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpF and GvpT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaFT
FEATURES             Location/Qualifiers
     source          1..7165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5423..5441)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5437..5484
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5551..5642
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6507..6546
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      6577..7165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7066..7085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaagtagaa gagaaataac
     2701 gtgcttcaca aattagtaac cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta
     2761 caggaagaag ctgataaaca gctatatgac cttccgacga ttcagcaaaa gctcattcag
     2821 cttcaaatga tgtttgagct tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat
     2881 gaattgttaa tgaggtacga aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta
     2941 acacaaaaaa gaaatgagga atcctagatg ggagaattac tgtatttata cggtttaatt
     3001 ccaacaaaag aagcagcagc catagagccg tttccatctt ataaggggtt tgacggagaa
     3061 cattcactgt acccaattgc gtttgatcag gtgacggctg tagtttctaa gctggatgct
     3121 gacacctatt cagaaaaagt gattcaagaa aaaatggagc aggatatgag ctggctgcag
     3181 gaaaaagcat ttcatcatca cgaaacggta gccgctttgt acgaagaatt tacgatcatt
     3241 ccattaaaat tttgcaccat ttataaaggt gaagaaagtc tgcaggcagc tattgagatt
     3301 aacaaagaaa agatagagaa ttcactgacg ctgcttcaag gaaatgaaga gtggaatgtg
     3361 aaaatttact gtgatgatac agagcttaaa aaaggaatca gcgaaacgaa tgaaagcgtg
     3421 aaagcgaaaa aacaagaaat tagtcactta tcaccaggaa gacagttttt tgaaaagaaa
     3481 aaaatagatc agctgattga aaaagaatta gagcttcaca aaaacaaagt gtgtgaagag
     3541 atacatgaca agctaaaaga attatcgctt tatgactctg ttaaaaagaa ttggagcaaa
     3601 gacgtaactg gcgcagctga acagatggcg tggaacagcg tgtttcttct cccgtctctg
     3661 cagattacta agttcgtaaa cgaaatagaa gagcttcagc aaaggcttga aaataaaggc
     3721 tggaagtttg aagtgacggg accatggccg ccctatcatt tctcgagctt tgcgtaaagt
     3781 gaggaattaa cattatgtct cttaaacaat ccatggagaa taaagatatt gctcttattg
     3841 atattttaga tgtcatttta gataaaggag tcgccattaa aggagactta atcatttcca
     3901 tagctggcgt cgatttagtg tatttggatt tgcgggtgct tatttcttcg gttgaaacgc
     3961 ttgtgcaagc aaaagaagga aatcacaaac caatcacttc tgaacaattt gataaacaaa
     4021 aggaggaatt aatggatgca accggtcagc caagcaaatg gacgaatcca cttggatcct
     4081 gatcaagctg aacaaggctt agcgcagctt gtgatgacag ttattgagct attgaggcaa
     4141 atcgttgaac gtcatgccat gaggcgggtg gagggtggaa cgttgacgga cgaacaaatt
     4201 gaaaacttag gaattgcact aatgaactta gaagaaaaaa tggacgagtt gaaagaggtg
     4261 ttcggtctgg atgcagaaga tttaaatatt gatcttggac cgctaggcag cctgctttaa
     4321 gcggtcagta ggaggaacag tatggcagtc gaacataata tgcagtcaag tacgattgta
     4381 gatgtgctcg aaaagatttt ggataaagga gtcgttatag cgggggacat caccgtagga
     4441 attgcagatg tcgagctatt aacgataaag atccgcttga ttgtggcttc ggttgataag
     4501 gcaaaagaaa tcggcatgga ctggtgggaa aatgatccgt atctcagttc aaaaggagcc
     4561 aataacaaag cgctcgaaga agaaaataaa atgctgcatg agcggttaaa aacgcttgaa
     4621 gaaaaaatag aaacgaaacg ttaaaaactg tacgctactt gaaaaacacg gacgaagaag
     4681 atgatgaagc aacatctctt tctagtgaag acgatacaac agcctaagac gtaaaggagg
     4741 aaagaaagac atgagtacag gcccttcttt ttcaactaaa gacaatacgc ttgaatactt
     4801 tgtgaaagct tctaataaac acggcttttc acttgatatt tcattaaatg taaacggcgc
     4861 tgtgatttcc ggtaccatga tttcagcaaa agaatacttt gattacttaa gcgaaacgtt
     4921 tgaagaaggc agtgaagtgg ctcaggcgct aagcgaacaa ttctctttag caagcgaagc
     4981 gagcgaatca aacggagaag cagaagccca ttttattcat ttgaaaaata caaagattta
     5041 ctgtggagac agtaaatcta ctccttctaa aggcaaaatc ttttggagag ggaaaatagc
     5101 agaagtagac gggtttttct taggaaagat ttctgatgca aaatcaacga gtaaaaagag
     5161 ttcataaaaa acggcggggt gattgccccg ccgtttttta gtgatgtgat gagatgtgca
     5221 gcttcttttt tcgatacatg agcggagtca tcttcatttc ttttcgaaac tgattaataa
     5281 aatagcttgt actattaaac cctacttgat aggcgacttc cgtcacattt gcttctgctt
     5341 gctgcagtcc ggattcgcga acgcgtctcg agagatccgg ctgctaacaa agcccgaaag
     5401 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     5461 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccgacta gtagcccgcc
     5521 taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata
     5581 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5641 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5701 ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga cgcagaggac caattggggg
     5761 cacgagtggg atacatagaa ctggacttga atagcggtaa aatccttgag agttttcgcc
     5821 ctgaagagcg ttttccaatg atgagcactt tcaaagttct gctatgtgga gcagtattat
     5881 cccgtgtaga tgcggggcaa gagcaactcg gacgacgaat acactattcg cagaatgact
     5941 tggttgaata ctccccagtg acagaaaagc accttacgga cggaatgacg gtaagagaat
     6001 tatgtagtgc cgccataacg atgagtgata acactgcggc gaacttactt ctgacaacca
     6061 tcggtggacc gaaggaatta accgcttttt tgcacaatat gggagaccat gtaactcgcc
     6121 ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga gacaccacaa
     6181 tgcctgcggc aatggcaaca acattacgca aactattaac tggcgaacta cttactctgg
     6241 cttcacggca acaattaata gactggcttg aagcggataa agttgcagga ccactactgc
     6301 gttcggcact tcctgctggc tggtttattg ctgataaatc tggggcagga gagcgtggtt
     6361 cacggggtat cattgccgca cttggaccag atggtaagcc ttcccgtatc gtagttatct
     6421 acacgacggg tagtcaggca actatggacg aacgaaatag acagattgct gaaatagggg
     6481 cttcactgat taagcattgg taaaccgata caattaaagg ctccttttgg agcctttttt
     6541 tttggacgga ccggtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6601 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6661 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6721 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6781 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6841 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6901 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6961 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7021 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7081 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7141 gtcagggggg cggagcctat ggaaa
//
