LOCUS       sequence-446030-        7670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpG and GvpL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaGL
FEATURES             Location/Qualifiers
     source          1..7670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3262..3279
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(5928..5946)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5942..5989
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6056..6147
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7012..7051
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7082..7670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7571..7590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttttagatc
     1441 taactattgg aggctactaa aaatggaaat taaaaaaatt atgcaagccg tgaacgactt
     1501 tttcggtgaa cacgtagctc ctcctcataa aattacctcg gtggaagcta ctgaagatga
     1561 aggttggaga gttattgttg aagtcattga agaacgagaa tatatgaaaa aatacgccaa
     1621 agatgaaatg ctcggaacgt acgagtgctt tgtaaataaa gaaaaagaag tcatttcatt
     1681 caaacgactc gacgtcagat atagaagcgc cattggcatt gaagcataaa cgataagatg
     1741 gcaggaggaa cgtaaaaatg accgtcttaa cagacaaaag gaaaaaaggc agtggagctt
     1801 ttatacaaga tgacgagaca aaagaggttc tttcaagagc gctgagctat ttaaaatccg
     1861 gctattccat tcattttaca ggtcctgccg gcggaggcaa aacctcttta gcgcgagcgc
     1921 ttgctaaaaa gagaaagcgt cctgtaatgc tgatgcacgg gaatcacgag ctcaacaaca
     1981 aagatttaat tggcgatttt acgggataca cgagcaaaaa agtaatcgac cagtacgttc
     2041 gttctgtcta taaaaaagat gaacaggtga gtgaaaactg gcaggatggc cgattgcttg
     2101 aagctgtaaa aaatggctat acgctgattt acgacgaatt tactcgttct aagcctgcga
     2161 cgaataatat ctttctatcg atattagaag aaggcgtgct gccgctgtat ggagtaaaaa
     2221 tgaccgatcc ttttgtgcgc gtgcatcccg atttccgcgt catcttcaca agcaatccag
     2281 ctgagtatgc cggcgtatat gatacgcaag atgcgcttct cgacaggtta attaccatgt
     2341 ttattgatta taaagacatc gacagagaga cagcgatttt aacggagaaa acggacgtag
     2401 aagaagatga agcgcgcaca attgtaacgc tcgtagcaaa cgtgcgaaac cgctctggag
     2461 acgaaaacag cagcggactt agcctgcggg cttcgcttat gatcgctacc cttgccacgc
     2521 agcaagacat tcctatcgat ggaagtgacg aagattttca aacgttatgt atcgatattt
     2581 tgcatcatcc gcttaccaaa tgtttggatg aagaaaatgc aaaaagcaaa gccgaaaaaa
     2641 tcattttaga agaatgtaag aatatagaca ctgaagaaaa gtaaaggagc ttgaaaacat
     2701 gagtgaaaca aacgaaacag gtatttatat ttttagcgcc attcaaacgg ataaagacga
     2761 agaatttggc gccgtggaag tagaaggaac aaaagctgaa acatttttga ttcgctacaa
     2821 agacgcggct atggtagcag ctgaagtacc gatgaaaatt tatcatccta atcgccaaaa
     2881 tttattaatg catcaaaacg cagtagcagc gattatggac aagaacgata cggttattcc
     2941 aatcagcttt gggaatgtat tcaaatcaaa agaagacgta aaagttcttt tggaaaacct
     3001 ttatccgcag tttgaaaagc tgtttccagc gatcaaagga aaaattgaag tcggtttaaa
     3061 agtaattggg aaaaaagaat ggcttgagaa aaaagtaaac gaaaatcctg aacttgagaa
     3121 agtatcagca tccgtaaaag gaaaatcaga agcagccggt tattatgagc gtattcaact
     3181 tggaggaatg gctcaaaaga tgtttacttc cctgcaaaaa gaagtcaaga cagatgtgtt
     3241 ttctccgctt gaagaagcag cggaagcagc aaaagcaaat gagccaacgg gcgaaacgat
     3301 gcttttaaac gcgtctttct taattaaccg agaagatgaa gcgaagtttg atgaaaaagt
     3361 aaatgaagcg catgaaaact ggaaagacaa agccgatttt cattacagcg gtccttggcc
     3421 tgcttataat tttgtgaaca ttcgcctaaa agtgaggaat taacattatg tctcttaaac
     3481 aatccatgga gaataaagat attgctctta ttgatatttt agatgtcatt ttagataaag
     3541 gagtcgccat taaaggagac ttaatcattt ccatagctgg cgtcgattta gtgtatttgg
     3601 atttgcgggt gcttatttct tcggttgaaa cgcttgtgca agcaaaagaa ggaaatcaca
     3661 aaccaatcac ttctgaacaa tttgataaac aaaaggagga attaatggat gcaaccggtc
     3721 agccaagcaa atggacgaat ccacttggat cctgatcaag ctgaacaagg cttagcgcag
     3781 cttgtgatga cagttattga gctattgagg caaatcgttg aacgtcatgc catgaggcgg
     3841 gtggagggtg gaacgttgac ggacgaacaa attgaaaact taggaattgc actaatgaac
     3901 ttagaagaaa aaatggacga gttgaaagag gtgttcggtc tggatgcaga agatttaaat
     3961 attgatcttg gaccgctagg cagcctgctt taagcggtca gtaggaggaa cagtatggca
     4021 gtcgaacata atatgcagtc aagtacgatt gtagatgtgc tcgaaaagat tttggataaa
     4081 ggagtcgtta tagcggggga catcaccgta ggaattgcag atgtcgagct attaacgata
     4141 aagatccgct tgattgtggc ttcggttgat aaggcaaaag aaatcggcat ggactggtgg
     4201 gaaaatgatc cgtatctcag ttcaaaagga gccaataaca aagcgctcga agaagaaaat
     4261 aaaatgctgc atgagcggtt aaaaacgctt gaagaaaaaa tagaaacgaa acgttaaaaa
     4321 ctgtacgcta cttaaaaaat ggagggattt acaatggcaa ctgaaacaaa attagataac
     4381 acacaggcag aaaacaagga aaataaaaat gcggaaaacg gttcaaaaga aaagaacggt
     4441 tcaaaagcaa gcaaaacaac aagcagcggg ccaatcaaac gagcggtagc aggaggcatc
     4501 atcggtgcaa cgattggata tgtatcgact cctgaaaatc gaaaaagtct ccttgaccgc
     4561 attgatacag acgaattaaa aagcaaagca tctgatttag gaacaaaggt aaaagaaaaa
     4621 tcaaaaagca gcgtggccag cctgaaaaca tctgcgggaa gcttgtttaa aaaagataaa
     4681 gataaatcaa aagatgatga agaaaacgta aattcttcta gtagcgaaac agaagacgat
     4741 aacgttcaag agtacgacga gttaaaagaa gaaaatcaaa ctcttcaaga tcgcttatca
     4801 cagcttgaag aaaaaatgaa catgcttgtt gagcttagcc tcaataaaaa tcaagacgaa
     4861 gaagcggaag atacagattc cgacgaagaa gagaacgatg agaacgatga aaacgatgaa
     4921 aacgagcagg acgatgaaaa cgaagaagaa acatctaagc cacgtaaaaa ggataaaaaa
     4981 gaagctgagg aagaagaaag tgaaagtgac gaagacagcg aggaagaaga ggaagattct
     5041 cgctcaaaca aaaaaaataa aaaagtaaaa acagaagaag aagacgaaga tgaaagcgaa
     5101 gaagaaaaaa aggaagcgaa accaaaaaag tcaacagcta aaaaatcaaa aaatacaaaa
     5161 gcaaagaaaa acacggacga agaagatgat gaagcaacat ctctttctag tgaagacgat
     5221 acaacagcct aagacgtaaa ggaggaaaga aagacatgag tacaggccct tctttttcaa
     5281 ctaaagacaa tacgcttgaa tactttgtga aagcttctaa taaacacggc ttttcacttg
     5341 atatttcatt aaatgtaaac ggcgctgtga tttccggtac catgatttca gcaaaagaat
     5401 actttgatta cttaagcgaa acgtttgaag aaggcagtga agtggctcag gcgctaagcg
     5461 aacaattctc tttagcaagc gaagcgagcg aatcaaacgg agaagcagaa gcccatttta
     5521 ttcatttgaa aaatacaaag atttactgtg gagacagtaa atctactcct tctaaaggca
     5581 aaatcttttg gagagggaaa atagcagaag tagacgggtt tttcttagga aagatttctg
     5641 atgcaaaatc aacgagtaaa aagagttcat aaaaaacggc ggggtgattg ccccgccgtt
     5701 ttttagtgat gtgatgagat gtgcagcttc ttttttcgat acatgagcgg agtcatcttc
     5761 atttcttttc gaaactgatt aataaaatag cttgtactat taaaccctac ttgataggcg
     5821 acttccgtca catttgcttc tgcttgctgc agtccggatt cgcgaacgcg tctcgagaga
     5881 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     5941 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     6001 aactatatcc gactagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
     6061 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6121 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6181 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta
     6241 aaagacgcag aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc
     6301 ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa
     6361 gttctgctat gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga
     6421 cgaatacact attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt
     6481 acggacggaa tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact
     6541 gcggcgaact tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac
     6601 aatatgggag accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata
     6661 ccaaacgacg agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta
     6721 ttaactggcg aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg
     6781 gataaagttg caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat
     6841 aaatctgggg caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt
     6901 aagccttccc gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga
     6961 aatagacaga ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt
     7021 aaaggctcct tttggagcct ttttttttgg acggaccggt agaaaagatc aaaggatctt
     7081 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7141 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7201 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7261 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7321 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7381 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7441 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7501 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7561 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7621 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
//
