LOCUS       sequence-446038-        7689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpR and GvpG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaRF
FEATURES             Location/Qualifiers
     source          1..7689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5947..5965)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5961..6008
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6075..6166
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7031..7070
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7101..7689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7590..7609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag cttcaggagg
     1441 aacgtaaaaa tgaccgtctt aacagacaaa aggaaaaaag gcagtggagc ttttatacaa
     1501 gatgacgaga caaaagaggt tctttcaaga gcgctgagct atttaaaatc cggctattcc
     1561 attcatttta caggtcctgc cggcggaggc aaaacctctt tagcgcgagc gcttgctaaa
     1621 aagagaaagc gtcctgtaat gctgatgcac gggaatcacg agctcaacaa caaagattta
     1681 attggcgatt ttacgggata cacgagcaaa aaagtaatcg accagtacgt tcgttctgtc
     1741 tataaaaaag atgaacaggt gagtgaaaac tggcaggatg gccgattgct tgaagctgta
     1801 aaaaatggct atacgctgat ttacgacgaa tttactcgtt ctaagcctgc gacgaataat
     1861 atctttctat cgatattaga agaaggcgtg ctgccgctgt atggagtaaa aatgaccgat
     1921 ccttttgtgc gcgtgcatcc cgatttccgc gtcatcttca caagcaatcc agctgagtat
     1981 gccggcgtat atgatacgca agatgcgctt ctcgacaggt taattaccat gtttattgat
     2041 tataaagaca tcgacagaga gacagcgatt ttaacggaga aaacggacgt agaagaagat
     2101 gaagcgcgca caattgtaac gctcgtagca aacgtgcgaa accgctctgg agacgaaaac
     2161 agcagcggac ttagcctgcg ggcttcgctt atgatcgcta cccttgccac gcagcaagac
     2221 attcctatcg atggaagtga cgaagatttt caaacgttat gtatcgatat tttgcatcat
     2281 ccgcttacca aatgtttgga tgaagaaaat gcaaaaagca aagccgaaaa aatcatttta
     2341 gaagaatgta agaatataga cactgaagaa aagtaagtag aagagaaata acgtgcttca
     2401 caaattagta accgcaccca ttaaccttgt agtgaaaatc ggcgaaaaag tacaggaaga
     2461 agctgataaa cagctatatg accttccgac gattcagcaa aagctcattc agcttcaaat
     2521 gatgtttgag cttggtgaaa ttccagaaga agcgtttcaa gaaaaagaag atgaattgtt
     2581 aatgaggtac gaaattgcga aacgcagaga aattgaacaa tgggaagagc taacacaaaa
     2641 aagaaatgag gaatcctaga tgggagaatt actgtattta tacggtttaa ttccaacaaa
     2701 agaagcagca gccatagagc cgtttccatc ttataagggg tttgacggag aacattcact
     2761 gtacccaatt gcgtttgatc aggtgacggc tgtagtttct aagctggatg ctgacaccta
     2821 ttcagaaaaa gtgattcaag aaaaaatgga gcaggatatg agctggctgc aggaaaaagc
     2881 atttcatcat cacgaaacgg tagccgcttt gtacgaagaa tttacgatca ttccattaaa
     2941 attttgcacc atttataaag gtgaagaaag tctgcaggca gctattgaga ttaacaaaga
     3001 aaagatagag aattcactga cgctgcttca aggaaatgaa gagtggaatg tgaaaattta
     3061 ctgtgatgat acagagctta aaaaaggaat cagcgaaacg aatgaaagcg tgaaagcgaa
     3121 aaaacaagaa attagtcact tatcaccagg aagacagttt tttgaaaaga aaaaaataga
     3181 tcagctgatt gaaaaagaat tagagcttca caaaaacaaa gtgtgtgaag agatacatga
     3241 caagctaaaa gaattatcgc tttatgactc tgttaaaaag aattggagca aagacgtaac
     3301 tggcgcagct gaacagatgg cgtggaacag cgtgtttctt ctcccgtctc tgcagattac
     3361 taagttcgta aacgaaatag aagagcttca gcaaaggctt gaaaataaag gctggaagtt
     3421 tgaagtgacg ggaccatggc cgccctatca tttctcgagc tttgcgtaaa gtgaggaatt
     3481 aacattatgt ctcttaaaca atccatggag aataaagata ttgctcttat tgatatttta
     3541 gatgtcattt tagataaagg agtcgccatt aaaggagact taatcatttc catagctggc
     3601 gtcgatttag tgtatttgga tttgcgggtg cttatttctt cggttgaaac gcttgtgcaa
     3661 gcaaaagaag gaaatcacaa accaatcact tctgaacaat ttgataaaca aaaggaggaa
     3721 ttaatggatg caaccggtca gccaagcaaa tggacgaatc cacttggatc ctgatcaagc
     3781 tgaacaaggc ttagcgcagc ttgtgatgac agttattgag ctattgaggc aaatcgttga
     3841 acgtcatgcc atgaggcggg tggagggtgg aacgttgacg gacgaacaaa ttgaaaactt
     3901 aggaattgca ctaatgaact tagaagaaaa aatggacgag ttgaaagagg tgttcggtct
     3961 ggatgcagaa gatttaaata ttgatcttgg accgctaggc agcctgcttt aagcggtcag
     4021 taggaggaac agtatggcag tcgaacataa tatgcagtca agtacgattg tagatgtgct
     4081 cgaaaagatt ttggataaag gagtcgttat agcgggggac atcaccgtag gaattgcaga
     4141 tgtcgagcta ttaacgataa agatccgctt gattgtggct tcggttgata aggcaaaaga
     4201 aatcggcatg gactggtggg aaaatgatcc gtatctcagt tcaaaaggag ccaataacaa
     4261 agcgctcgaa gaagaaaata aaatgctgca tgagcggtta aaaacgcttg aagaaaaaat
     4321 agaaacgaaa cgttaaaaac tgtacgctac ttaaaaaatg gagggattta caatggcaac
     4381 tgaaacaaaa ttagataaca cacaggcaga aaacaaggaa aataaaaatg cggaaaacgg
     4441 ttcaaaagaa aagaacggtt caaaagcaag caaaacaaca agcagcgggc caatcaaacg
     4501 agcggtagca ggaggcatca tcggtgcaac gattggatat gtatcgactc ctgaaaatcg
     4561 aaaaagtctc cttgaccgca ttgatacaga cgaattaaaa agcaaagcat ctgatttagg
     4621 aacaaaggta aaagaaaaat caaaaagcag cgtggccagc ctgaaaacat ctgcgggaag
     4681 cttgtttaaa aaagataaag ataaatcaaa agatgatgaa gaaaacgtaa attcttctag
     4741 tagcgaaaca gaagacgata acgttcaaga gtacgacgag ttaaaagaag aaaatcaaac
     4801 tcttcaagat cgcttatcac agcttgaaga aaaaatgaac atgcttgttg agcttagcct
     4861 caataaaaat caagacgaag aagcggaaga tacagattcc gacgaagaag agaacgatga
     4921 gaacgatgaa aacgatgaaa acgagcagga cgatgaaaac gaagaagaaa catctaagcc
     4981 acgtaaaaag gataaaaaag aagctgagga agaagaaagt gaaagtgacg aagacagcga
     5041 ggaagaagag gaagattctc gctcaaacaa aaaaaataaa aaagtaaaaa cagaagaaga
     5101 agacgaagat gaaagcgaag aagaaaaaaa ggaagcgaaa ccaaaaaagt caacagctaa
     5161 aaaatcaaaa aatacaaaag caaagaaaaa cacggacgaa gaagatgatg aagcaacatc
     5221 tctttctagt gaagacgata caacagccta agacgtaaag gaggaaagaa agacatgagt
     5281 acaggccctt ctttttcaac taaagacaat acgcttgaat actttgtgaa agcttctaat
     5341 aaacacggct tttcacttga tatttcatta aatgtaaacg gcgctgtgat ttccggtacc
     5401 atgatttcag caaaagaata ctttgattac ttaagcgaaa cgtttgaaga aggcagtgaa
     5461 gtggctcagg cgctaagcga acaattctct ttagcaagcg aagcgagcga atcaaacgga
     5521 gaagcagaag cccattttat tcatttgaaa aatacaaaga tttactgtgg agacagtaaa
     5581 tctactcctt ctaaaggcaa aatcttttgg agagggaaaa tagcagaagt agacgggttt
     5641 ttcttaggaa agatttctga tgcaaaatca acgagtaaaa agagttcata aaaaacggcg
     5701 gggtgattgc cccgccgttt tttagtgatg tgatgagatg tgcagcttct tttttcgata
     5761 catgagcgga gtcatcttca tttcttttcg aaactgatta ataaaatagc ttgtactatt
     5821 aaaccctact tgataggcga cttccgtcac atttgcttct gcttgctgca gtccggattc
     5881 gcgaacgcgt ctcgagagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     5941 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     6001 gttttttgct gaaaggagga actatatccg actagtagcc cgcctaatga gcgggctttt
     6061 ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6181 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6241 agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag tgggatacat
     6301 agaactggac ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag agcgttttcc
     6361 aatgatgagc actttcaaag ttctgctatg tggagcagta ttatcccgtg tagatgcggg
     6421 gcaagagcaa ctcggacgac gaatacacta ttcgcagaat gacttggttg aatactcccc
     6481 agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta gtgccgccat
     6541 aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg gaccgaagga
     6601 attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc gttgggaacc
     6661 agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg cggcaatggc
     6721 aacaacatta cgcaaactat taactggcga actacttact ctggcttcac ggcaacaatt
     6781 aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg cacttcctgc
     6841 tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg gtatcattgc
     6901 cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga cgggtagtca
     6961 ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac tgattaagca
     7021 ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga cggaccggta
     7081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7681 ctatggaaa
//
