LOCUS       sequence-446039-        8207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mega' with knockout of GvpR and GvpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Iburg M, Anderson AP, Wong VT, Anton ED, He A, Lu GJ
  TITLE     Elucidating the assembly of gas vesicles by systematic 
            protein-protein interaction analysis.
  JOURNAL   EMBO J. 2024 Oct;43(19):4156-4172. doi: 10.1038/s44318-024-00178-2. 
            Epub 2024 Sep 3.
  PUBMED    39227754
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00178-2"; journalName: "EMBO J"; date: 
            "2024-10"; volume: "43"; issue: "19"; pages: "4156-4172"
COMMENT     Sequence Label: pST39-Mega'-deltaRG
FEATURES             Location/Qualifiers
     source          1..8207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(251..270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     411..433
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(427..618)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2955..2972
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(6465..6483)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6479..6526
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6593..6684
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7549..7588
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      7619..8207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8108..8127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
      241 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      301 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      361 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      421 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      481 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
      541 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
      601 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
      661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
      721 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
      781 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttgg caccatgcat
      841 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaga
      901 tagcttagcg atgtgaaaga agctggagaa aactttcaag aaaaagtttc ttcttcagac
      961 gataaagaga agtcaaaaaa taagcggaaa ataaaaggcg taaatcagat taaaaaagca
     1021 acggatatta aaagctctac taagattaaa agttctaatg atattaaatc atctacagat
     1081 ttaaaaacaa tgggatcata agcgaaagga gaaatgagat atgtctattc aaaaaagtac
     1141 taatagttca agtttagcag aagtcattga ccgtatttta gataaaggaa ttgttattga
     1201 tgcttttgca agagtttctg ttgtaggaat tgaaatttta acgattgaag cgcgagtggt
     1261 tattgccagt gttgatacat ggttacgcta tgcagaagca gtagggcttc ttcgtgacga
     1321 cgtagaagaa aacggtcttc ctgaacgttc aaattcaagt gaagggcagc cgcgttttag
     1381 tatttaatat gaagtggtat ggaaccttcc ttttttgagg gtggttccag ctttcaggag
     1441 gaacgtaaaa atgaccgtct taacagacaa aaggaaaaaa ggcagtggag cttttataca
     1501 agatgacgag acaaaagagg ttctttcaag agcgctgagc tatttaaaat ccggctattc
     1561 cattcatttt acaggtcctg ccggcggagg caaaacctct ttagcgcgag cgcttgctaa
     1621 aaagagaaag cgtcctgtaa tgctgatgca cgggaatcac gagctcaaca acaaagattt
     1681 aattggcgat tttacgggat acacgagcaa aaaagtaatc gaccagtacg ttcgttctgt
     1741 ctataaaaaa gatgaacagg tgagtgaaaa ctggcaggat ggccgattgc ttgaagctgt
     1801 aaaaaatggc tatacgctga tttacgacga atttactcgt tctaagcctg cgacgaataa
     1861 tatctttcta tcgatattag aagaaggcgt gctgccgctg tatggagtaa aaatgaccga
     1921 tccttttgtg cgcgtgcatc ccgatttccg cgtcatcttc acaagcaatc cagctgagta
     1981 tgccggcgta tatgatacgc aagatgcgct tctcgacagg ttaattacca tgtttattga
     2041 ttataaagac atcgacagag agacagcgat tttaacggag aaaacggacg tagaagaaga
     2101 tgaagcgcgc acaattgtaa cgctcgtagc aaacgtgcga aaccgctctg gagacgaaaa
     2161 cagcagcgga cttagcctgc gggcttcgct tatgatcgct acccttgcca cgcagcaaga
     2221 cattcctatc gatggaagtg acgaagattt tcaaacgtta tgtatcgata ttttgcatca
     2281 tccgcttacc aaatgtttgg atgaagaaaa tgcaaaaagc aaagccgaaa aaatcatttt
     2341 agaagaatgt aagaatatag acactgaaga aaagtaaagg agcttgaaaa catgagtgaa
     2401 acaaacgaaa caggtattta tatttttagc gccattcaaa cggataaaga cgaagaattt
     2461 ggcgccgtgg aagtagaagg aacaaaagct gaaacatttt tgattcgcta caaagacgcg
     2521 gctatggtag cagctgaagt accgatgaaa atttatcatc ctaatcgcca aaatttatta
     2581 atgcatcaaa acgcagtagc agcgattatg gacaagaacg atacggttat tccaatcagc
     2641 tttgggaatg tattcaaatc aaaagaagac gtaaaagttc ttttggaaaa cctttatccg
     2701 cagtttgaaa agctgtttcc agcgatcaaa ggaaaaattg aagtcggttt aaaagtaatt
     2761 gggaaaaaag aatggcttga gaaaaaagta aacgaaaatc ctgaacttga gaaagtatca
     2821 gcatccgtaa aaggaaaatc agaagcagcc ggttattatg agcgtattca acttggagga
     2881 atggctcaaa agatgtttac ttccctgcaa aaagaagtca agacagatgt gttttctccg
     2941 cttgaagaag cagcggaagc agcaaaagca aatgagccaa cgggcgaaac gatgctttta
     3001 aacgcgtctt tcttaattaa ccgagaagat gaagcgaagt ttgatgaaaa agtaaatgaa
     3061 gcgcatgaaa actggaaaga caaagccgat tttcattaca gcggtccttg gcctgcttat
     3121 aattttgtga acattcgcct aaaagtagaa gagaaataac gaaatgagga atcctagatg
     3181 ggagaattac tgtatttata cggtttaatt ccaacaaaag aagcagcagc catagagccg
     3241 tttccatctt ataaggggtt tgacggagaa cattcactgt acccaattgc gtttgatcag
     3301 gtgacggctg tagtttctaa gctggatgct gacacctatt cagaaaaagt gattcaagaa
     3361 aaaatggagc aggatatgag ctggctgcag gaaaaagcat ttcatcatca cgaaacggta
     3421 gccgctttgt acgaagaatt tacgatcatt ccattaaaat tttgcaccat ttataaaggt
     3481 gaagaaagtc tgcaggcagc tattgagatt aacaaagaaa agatagagaa ttcactgacg
     3541 ctgcttcaag gaaatgaaga gtggaatgtg aaaatttact gtgatgatac agagcttaaa
     3601 aaaggaatca gcgaaacgaa tgaaagcgtg aaagcgaaaa aacaagaaat tagtcactta
     3661 tcaccaggaa gacagttttt tgaaaagaaa aaaatagatc agctgattga aaaagaatta
     3721 gagcttcaca aaaacaaagt gtgtgaagag atacatgaca agctaaaaga attatcgctt
     3781 tatgactctg ttaaaaagaa ttggagcaaa gacgtaactg gcgcagctga acagatggcg
     3841 tggaacagcg tgtttcttct cccgtctctg cagattacta agttcgtaaa cgaaatagaa
     3901 gagcttcagc aaaggcttga aaataaaggc tggaagtttg aagtgacggg accatggccg
     3961 ccctatcatt tctcgagctt tgcgtaaagt gaggaattaa cattatgtct cttaaacaat
     4021 ccatggagaa taaagatatt gctcttattg atattttaga tgtcatttta gataaaggag
     4081 tcgccattaa aggagactta atcatttcca tagctggcgt cgatttagtg tatttggatt
     4141 tgcgggtgct tatttcttcg gttgaaacgc ttgtgcaagc aaaagaagga aatcacaaac
     4201 caatcacttc tgaacaattt gataaacaaa aggaggaatt aatggatgca accggtcagc
     4261 caagcaaatg gacgaatcca cttggatcct gatcaagctg aacaaggctt agcgcagctt
     4321 gtgatgacag ttattgagct attgaggcaa atcgttgaac gtcatgccat gaggcgggtg
     4381 gagggtggaa cgttgacgga cgaacaaatt gaaaacttag gaattgcact aatgaactta
     4441 gaagaaaaaa tggacgagtt gaaagaggtg ttcggtctgg atgcagaaga tttaaatatt
     4501 gatcttggac cgctaggcag cctgctttaa gcggtcagta ggaggaacag tatggcagtc
     4561 gaacataata tgcagtcaag tacgattgta gatgtgctcg aaaagatttt ggataaagga
     4621 gtcgttatag cgggggacat caccgtagga attgcagatg tcgagctatt aacgataaag
     4681 atccgcttga ttgtggcttc ggttgataag gcaaaagaaa tcggcatgga ctggtgggaa
     4741 aatgatccgt atctcagttc aaaaggagcc aataacaaag cgctcgaaga agaaaataaa
     4801 atgctgcatg agcggttaaa aacgcttgaa gaaaaaatag aaacgaaacg ttaaaaactg
     4861 tacgctactt aaaaaatgga gggatttaca atggcaactg aaacaaaatt agataacaca
     4921 caggcagaaa acaaggaaaa taaaaatgcg gaaaacggtt caaaagaaaa gaacggttca
     4981 aaagcaagca aaacaacaag cagcgggcca atcaaacgag cggtagcagg aggcatcatc
     5041 ggtgcaacga ttggatatgt atcgactcct gaaaatcgaa aaagtctcct tgaccgcatt
     5101 gatacagacg aattaaaaag caaagcatct gatttaggaa caaaggtaaa agaaaaatca
     5161 aaaagcagcg tggccagcct gaaaacatct gcgggaagct tgtttaaaaa agataaagat
     5221 aaatcaaaag atgatgaaga aaacgtaaat tcttctagta gcgaaacaga agacgataac
     5281 gttcaagagt acgacgagtt aaaagaagaa aatcaaactc ttcaagatcg cttatcacag
     5341 cttgaagaaa aaatgaacat gcttgttgag cttagcctca ataaaaatca agacgaagaa
     5401 gcggaagata cagattccga cgaagaagag aacgatgaga acgatgaaaa cgatgaaaac
     5461 gagcaggacg atgaaaacga agaagaaaca tctaagccac gtaaaaagga taaaaaagaa
     5521 gctgaggaag aagaaagtga aagtgacgaa gacagcgagg aagaagagga agattctcgc
     5581 tcaaacaaaa aaaataaaaa agtaaaaaca gaagaagaag acgaagatga aagcgaagaa
     5641 gaaaaaaagg aagcgaaacc aaaaaagtca acagctaaaa aatcaaaaaa tacaaaagca
     5701 aagaaaaaca cggacgaaga agatgatgaa gcaacatctc tttctagtga agacgataca
     5761 acagcctaag acgtaaagga ggaaagaaag acatgagtac aggcccttct ttttcaacta
     5821 aagacaatac gcttgaatac tttgtgaaag cttctaataa acacggcttt tcacttgata
     5881 tttcattaaa tgtaaacggc gctgtgattt ccggtaccat gatttcagca aaagaatact
     5941 ttgattactt aagcgaaacg tttgaagaag gcagtgaagt ggctcaggcg ctaagcgaac
     6001 aattctcttt agcaagcgaa gcgagcgaat caaacggaga agcagaagcc cattttattc
     6061 atttgaaaaa tacaaagatt tactgtggag acagtaaatc tactccttct aaaggcaaaa
     6121 tcttttggag agggaaaata gcagaagtag acgggttttt cttaggaaag atttctgatg
     6181 caaaatcaac gagtaaaaag agttcataaa aaacggcggg gtgattgccc cgccgttttt
     6241 tagtgatgtg atgagatgtg cagcttcttt tttcgataca tgagcggagt catcttcatt
     6301 tcttttcgaa actgattaat aaaatagctt gtactattaa accctacttg ataggcgact
     6361 tccgtcacat ttgcttctgc ttgctgcagt ccggattcgc gaacgcgtct cgagagatcc
     6421 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     6481 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     6541 tatatccgac tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta
     6601 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6661 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6721 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctcgt gaaagtaaaa
     6781 gacgcagagg accaattggg ggcacgagtg ggatacatag aactggactt gaatagcggt
     6841 aaaatccttg agagttttcg ccctgaagag cgttttccaa tgatgagcac tttcaaagtt
     6901 ctgctatgtg gagcagtatt atcccgtgta gatgcggggc aagagcaact cggacgacga
     6961 atacactatt cgcagaatga cttggttgaa tactccccag tgacagaaaa gcaccttacg
     7021 gacggaatga cggtaagaga attatgtagt gccgccataa cgatgagtga taacactgcg
     7081 gcgaacttac ttctgacaac catcggtgga ccgaaggaat taaccgcttt tttgcacaat
     7141 atgggagacc atgtaactcg ccttgaccgt tgggaaccag aactgaatga agccatacca
     7201 aacgacgagc gagacaccac aatgcctgcg gcaatggcaa caacattacg caaactatta
     7261 actggcgaac tacttactct ggcttcacgg caacaattaa tagactggct tgaagcggat
     7321 aaagttgcag gaccactact gcgttcggca cttcctgctg gctggtttat tgctgataaa
     7381 tctggggcag gagagcgtgg ttcacggggt atcattgccg cacttggacc agatggtaag
     7441 ccttcccgta tcgtagttat ctacacgacg ggtagtcagg caactatgga cgaacgaaat
     7501 agacagattg ctgaaatagg ggcttcactg attaagcatt ggtaaaccga tacaattaaa
     7561 ggctcctttt ggagcctttt tttttggacg gaccggtaga aaagatcaaa ggatcttctt
     7621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7741 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8101 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8161 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaa
//
