LOCUS       sequence-446049-       10785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of KIF 13a WLDLE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Mendoza CS, Plowinske CR, Montgomery AC, Quinones GB, Banker G, 
            Bentley M
  TITLE     Kinesin Regulation in the Proximal Axon is Essential for 
            Dendrite-selective Transport.
  JOURNAL   Mol Biol Cell. 2024 Jun 1;35(6):ar81. doi: 10.1091/mbc.E23-11-0457. 
            Epub 2024 Apr 10.
  PUBMED    38598291
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-06-1"; volume: "35(6):ar81. doi"; pages: " 
            10.1091/mbc.E23-11-0457. Epub 2024 Apr 10"
COMMENT     Sequence Label: pBa.GFP-KIF13AWLDLE
FEATURES             Location/Qualifiers
     source          1..10785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(153..172)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     500..521
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      4338..4347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5955..6089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5992..6011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6046..6065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6120..6136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6120..6136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6133..6155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6144..6160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6168..6174,6175..6192,6193..6198))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6213..6234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6351..6368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6522..7110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6602..6621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7281..8072,8073..8141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7904..7923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8142..8246)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8314..8332
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8370..8392)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8492..8511
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8705..8727
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8719..8736
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8720..8736
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcatgctc ctggtgttat tgcagcctgg tgcatgcatg cgtcttgatg gtgcagcgct
       61 gtgcatgcat cctccttggt gtgtagcagt ctagtgcatg catacccctc ggtgttattg
      121 ctgctctcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      181 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      241 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      301 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      361 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      421 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
      481 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      541 tcggcatgga cgagctgtac ctgacgcgtg gaggagctgg aataggcatg tcggatacga
      601 aggtaaaagt tgccgtccgg gtccggccca tgaaccgaag agagctggaa ctgaacacca
      661 agtgcgtggt ggagatggaa gggaaccaga cggtcctgca ccctcctcct tccaacacta
      721 agcagggaga aagaaaacct cccaaggtgt ttgcctttga ttattgcttt tggtccatgg
      781 atgaatcgaa cacaacaaaa tatgctggtc aagaagtcgt tttcaagtgc ctcggggaag
      841 gaattctcga gaaagccttt cagggataca atgcttgtat ttttgcatat ggccagacag
      901 gttctggaaa gtccttttcc atgatgggcc acgcagagca gctgggcctt atcccaaggc
      961 tctgctgtgc tctgtttcaa aggatcgccc tggagcaaaa tgagtcacag accttcaaag
     1021 tcgaggtatc gtatatggag atctataacg agaaggtccg ggacctcttg gaccccaaag
     1081 ggagcaggca gtctctgaaa gtccgagagc ataaggtcct ggggccgtat gtcgatggtc
     1141 tgtctcagct ggctgtcacc agctttgagg acattgagtc actgatgtct gagggaaata
     1201 agtctcgaac ggtagcagcg accaacatga atgaggagag cagccgttcc catgcggtct
     1261 tcaacatcat catcacacag acgctgtatg acctacagtc tgggaactcc ggggagaagg
     1321 tcagcaaggt gagcctggta gacctggcgg ggagcgagag agtgtcgaag actggagctg
     1381 cgggtgagcg gctgaaagaa ggcagcaaca ttaacaaatc ccttacaacc ttgggattgg
     1441 ttatatcgtc actggctgac caggctgctg gcaaaggcaa aaacaaattc gtgccttatc
     1501 gagattcggt ccttacttgg cttcttaagg acaatttagg agggaacagc cagacctcta
     1561 tgatagcgac catcagccca gcagcagaca actatgagga gacactgtcc acgctaagat
     1621 atgcagatcg agccaagagg attgtgaacc acgctgtggt gaatgaggat cccaatgcca
     1681 aggtcatccg agagcttcga gaggaagtgg agaaactgag ggagcagctc tcgcaggctg
     1741 aggccatgaa agcccccgag ctaaaggaga agctcgaaga gtctgagaag ctgataaagg
     1801 agctaacggt gacgtgggaa gagaagctga ggaaaacaga agcgatcgca caggagagac
     1861 agcggcagct ggaaagcatg ggaatctccc tggagacctc tggcatcaag gtgggggacg
     1921 acaagtgcta cctcgtcaac ctgaacgcag accctgccct caacgagctt ctggtttact
     1981 atttaaagga tcatacccgg gtgggtgcag atacctctca ggacatccag ctcttcggaa
     2041 tagggattca gcccgagcac tgtgagatcg acatcgcagc ggatggagac atcactctca
     2101 cgcccaaaga aaatgcaagg tcctgtggca atggcaccct tgtctgcagt actacccagc
     2161 tgtggcacgg cgacaggatc ttatggggaa acaaccactt ttttagaatt aacttgccta
     2221 agaggaaacg gcgggactgg ttgaaagact ttgagagaga aaccagttcc gcggagcacg
     2281 atctagacgc tgcgagcgag gcttcctcgg agccagacta caactacgag ttcgcacaga
     2341 tggaggtcat catgaaaaca ctgaacagca atgatccagt tcaaaatgtg gttcaggtcc
     2401 tggagaagca gtacctggaa gagaagcgga ccgccctgga ggaacagcgg ctcatgtacg
     2461 aacgcgagct ggagcagctt cgccagcagc tctctcccga gaggcagccg cccagcagcg
     2521 cctcagaccg tctggcctac agcagccaga cagcccagca gaaggtgacc cagtgggccg
     2581 aggagaggga tgaactcttt aggcagagct tggccaagct tcgagagcag ctggtgaaag
     2641 ccaacacttt ggtgagggaa gccaacttcc tagccgagga gatgagcaaa ctcactgact
     2701 accaagtgac tctgcagatc cctgcggcca acctcagtgc caacaggaag agaggcgcaa
     2761 tcgtcagcga gcccgccatc caagccagga ggaaagggaa gggcacccag gtctggacca
     2821 ttgagaagct ggagaataag ctaattgata tgagagacct ttaccaagaa tggaaagaaa
     2881 atgttcctga ggccaagagg ctctatggga agcgaggtga ccctttttat gaagcgcagg
     2941 agaatcacaa cctcattggt gtggctaatg tgtttttgga atgtctcttc tgtgatgtga
     3001 agcttcaata tgcagtaccc atcatcagcc agcaggggga ggttgccggg cgcctccatg
     3061 tggaggtgac gcgcattaca ggaaccattc cagaacgcat ggcggaggat gactcttcag
     3121 agaactctag tgagagcggg agtcttgaag tggtcgacag cagcggcgaa gtcattcacc
     3181 gggtcaaaaa gctgacatgc agggtgataa ttaaagaggc aaccgggcta cccataagcc
     3241 tctcaaattt cgtcttctgt cagtacacgt tctgggacca gtgtgagtct acagtggctg
     3301 ctccagtggt ggacccagat gtgccctccc cgcagtccaa ggacgcacag tacacggtga
     3361 ccttctctca ctgtaaggac tacgtggtga ctgtcacaga agagttcctg gagttcattt
     3421 ctgatggtgc gctggcgatt gaagtgtggg gccacaggtg tgctgggaac ggaagcccca
     3481 tctgggaggt ggactcgctc catgcgaaga caaggacatt gcatgacagg tggaatgaag
     3541 tgacccggag aatagaggtg tggatctcca ttctggagct gaatgagctc ggggattatg
     3601 ccgctgtgga gcttcaccag gcgaaagacg tcaacaccgg cggcgtcttc caactcagac
     3661 agggtcactc ccgtagagtg caggtcacag tgaaacctgt acagcactca gggacactgc
     3721 cactcatggt tgaagccatc ctatcagtat ccattggctg cgtgaccgcc aggtccacca
     3781 agctgcagag agggctggac agctaccagg aagaagactt gaattgtgta agagagagat
     3841 ggtccgatgc actcattaaa cgacgggaat acttagacga acagattaaa aaagtcagca
     3901 ataaaaaaga gaaaacagaa gacgacatgg agcgggaagc caggctggtg gagcagtggg
     3961 tggggctgac agaggagagg aatgctgtgc tggtgcccgc ccctggcagc ggaatccccg
     4021 gggcacctgc tgactgggtc ccacctcctg gcatggaaac acacatcccg gttctcttcc
     4081 tggatctgaa cgcggatgac ctcagtgcca atgagcagct ggtgggcccc catgcgtccg
     4141 gtgtgaactc catcctgccc aaggaacacg gcagtcagtt tttctacctg cccatcataa
     4201 agcacagtga tgacgaggtt tcagccacag cctcctggga ctcctctgtg cacgactctc
     4261 ttcacctgaa tcgggtcaca ccgcagaatg aaaggatcta cctgatcgtg aagaccaccg
     4321 tgcagctcag ccacccggcc gccatggagc tagtgttgcg gaagcggatc gcggccaata
     4381 tttataacaa gcagagtttc acccagagtt tgaagagacg aatatcactg atcaatatac
     4441 gttactcctg cggcgtgacc tatgagatag tatccaatat cccaaaggcg accgaggaga
     4501 ttgaggaccg ggaaaccctg gccctcctgg cagcgcggag tgagaacgag ggcactttgg
     4561 atggggagac atacatcgag aagtacacgc ggggcgtgct gcaggtggag aatatcctga
     4621 gcctggagcg gctccggcag gctgtcacgg tgaaggaagc gctttccacc aaagcgaggc
     4681 acattcggtg gctcgacctc gagaatgttc acaacgtctc ctctagccgg ccagatctct
     4741 ctggcttcga tgaagacgat aagggctggc ccgagaacca gttagacgtg tctgactaca
     4801 gctccagcta ccaagacgtc gcgtgctatg ggactttacc cagggactca cctcgcagga
     4861 gcaaggaagg ttgtccatca gagaatcctc atgccttaac agtcagccct tttaaagcgt
     4921 tctctcctca gccaccaaag ttcttcaaac ccctcatgcc tgtaaaagag gagcataaga
     4981 aaaggttggc cctggaagcc agacctctcc taagccaaga ggactcggaa gaggaggaaa
     5041 acgagctgga agctctcagc aggaagctga tgcttacaca gccttatgtg cccgtggagt
     5101 tcgctgactt cagtgtttac aatgccagct tggagaacag ggagtggtcc tcttctaagg
     5161 cggacctgac agactcgaga gccttggaga aagcagtgtc ccgtagccct accaccagca
     5221 gcctcaccag tggctacttc tcccacagtg cctccaatgc cactctgtct gacatggcag
     5281 tcccctccag cgacagctca gaccagcttg ccgtctcgac aaaggaggtc gagtgcgccg
     5341 agcccccggg accatcactt gcaccggatg tcagacgagc ttcgaatcag gagcttacag
     5401 aagtaggaag aggctcaggg aaggatgaga caatcgctgt gccgctggag gaaaacagtg
     5461 ccttacccaa ggggaccccg tcaccccaga gcatccctga ggaaagttcc agaatgccat
     5521 gcaggactgc ttcatgttca gaactggatg tcggccccag caaagatggc caccaagcca
     5581 gagaattctg cccaggagag gtgacgatag aacacactac aaacattttg gaggaccatt
     5641 ccttcacaga gttcatgggt gtttctgatg gcaaagactt tgatggtttg gcagattgtt
     5701 ctgtagggga gccctccagg aggagggctc taacaaacga aacagaccat aaaggtatcc
     5761 cagaaagacc tccagatgcc gaccggctgc atcccaagat agagaacgac caggaagcca
     5821 cagccactcg gtaagctaga taagtaatga tcataatcag ccatatcaca tctgtagagg
     5881 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     5941 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6001 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6061 tcatcaatgt atcttatcat gtctggatcc ccgggtaccg agctcgaatt cgtaatcatg
     6121 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6181 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6241 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6301 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     6361 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6421 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6481 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6541 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6601 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6661 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6721 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6781 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6841 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6901 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6961 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7141 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7201 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7321 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7381 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7441 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7501 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7561 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7621 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7681 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7741 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7801 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7861 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7921 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7981 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8041 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8101 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     8281 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     8341 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     8401 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     8461 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     8521 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     8581 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8641 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8701 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agttgggatc tttgcattgg
     8761 cccacggctc tcaggatggg gatgctcccc ttcagcaccc ggttcccctt ggaaactgat
     8821 ggtcctggct ctgtggcatg gcagtggcac tgtgaggagc cccctaccag cagcacacag
     8881 tggggttggc actgccacgc tccggatgcc gcgctctgat ccaaccccat aatcaaggga
     8941 acccgaattg ccccatcatt gcccccacca cccccatcct gccgggccct cacaccccac
     9001 gctgccttct ggtgacattc cccagcccaa acccacggct tcatggctac cgcggggcat
     9061 ttcccattgc cgccccatta tcagctctgc acacctcccg ctgtacccat gcctcgtggc
     9121 tgcccttctt tgacgtataa tcttctaatt aatacccggc cttgtcaaag tggagcacaa
     9181 acgttaatta attccccagc aggcaggtaa ttaacagtgt gactcccttt ttgctgcgag
     9241 tggggctgat acagagagat gtggcactat ggagcccacg gggtcctggc actgggtgcc
     9301 cacggaggtc cccatgtgct gcagtgtcac cgcctccgag gagacagtat tgtccctgcg
     9361 gtgtccctgc agctcagctc tgtccacagg gccacctcca gtttggaggg gacacaatgc
     9421 agccccgatg caacccatcc tcgcagcatc ccagggacaa agaccccact gcaagaccgc
     9481 acacagggct gggtcccgct cccctaatat ctacagtgct tcttgcatgg ccccttaatc
     9541 aatgcagtta atcagcatgc gctcatgcac cgctctggag ctgcaaagcc cctcgcagcg
     9601 ctgctcacca acaccgcgca ccgccccggc ccagcctgca gcacgcgctg caaacaggaa
     9661 agaaacaaaa tattgcccaa atgtaggcaa aggcattcgg ctgccttgac ctccgccggg
     9721 ccgggccctg cctgactcag ctccttactc agcgctcgct tcctccctcc ggctgccacc
     9781 gccgcagcgc acaccctgac aaagagtggc ccttaacggg ctctgaggtg cacccagcag
     9841 tgcactcagc agtccaaggg ccggcctgga ggtttgcacc gctacgtgct gacattagca
     9901 ttgaacttgg ccctgggtag tgctgcaggc cgggcggggt gggtgtagag agtgcagcgc
     9961 gcgttgcacc cggtgcccct tcccctccct tgcatcccag caggctgcac cccagcacca
    10021 ggcccgtgca tgcatgctcc tggtgttatt gcagcctggt gcatgcatgc gtcttgatgg
    10081 tgcagcgctg tgcatgcatc ctccttggtg tgtagcagtc tagtgcatgc atacccctcg
    10141 gtgttattgc tgctctgtgc acgcacgctc attgtatcac ttcatcccag tgcatgcact
    10201 cacactggag cgattgctgc tcggtgcacg cacactcatt gtatcactgc agctcagtgg
    10261 ctgcacgcac accggtgtta ttgctgctcg gtgcgtgcat gcacatcagt gtcgctgcag
    10321 ctcagtgcat gcacgctcat tgcccatcgc tatccctgcc tctcctgctg gcgctccccg
    10381 ggaggtgact tcaaggggac cgcaggacca cctcgggggt ggggggaggg ctgcacacgc
    10441 ggaccccgct ccccctcccc aacaaagcac tgtggaatca aaaagggggg aggggggatg
    10501 gaggggcgcg tcacaccccc gccccacacc ctcacctcga ggtgagcccc acgttctgct
    10561 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
    10621 tattttgtgc agcgatgggg gcgggggggg gggggggggg ggccgggggg gggggggggg
    10681 gggggggggg gggggggggg gggggggggg gggggggggg gggggcgggc gggggggggg
    10741 gggggggggg ggggggggtg gggggggggg gggggggggg ggggg
//
