LOCUS       sequence-446053-        9285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of MARK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Mendoza CS, Plowinske CR, Montgomery AC, Quinones GB, Banker G, 
            Bentley M
  TITLE     Kinesin Regulation in the Proximal Axon is Essential for 
            Dendrite-selective Transport.
  JOURNAL   Mol Biol Cell. 2024 Jun 1;35(6):ar81. doi: 10.1091/mbc.E23-11-0457. 
            Epub 2024 Apr 10.
  PUBMED    38598291
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-06-1"; volume: "35(6):ar81. doi"; pages: " 
            10.1091/mbc.E23-11-0457. Epub 2024 Apr 10"
COMMENT     Sequence Label: pBa.MARK2-Halo
FEATURES             Location/Qualifiers
     source          1..9285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(483..1274,1275..1343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1106..1125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1344..1448)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1516..1534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1572..1594)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1694..1713
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1907..1929
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1921..1938
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1922..1938
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5070..5092
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    5157..5181
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7416..8300
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     polyA_signal    8442..8576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8479..8498)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8533..8552
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8607..8623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8607..8623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8620..8642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8631..8647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8655..8661,8662..8679,8680..8685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8700..8721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8838..8855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9009..9285,1..312))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9089..9108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
       61 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
      541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
      601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
      661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
      721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
      781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
      841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
      901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
      961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1441 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     1501 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     1561 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1621 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1681 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     1741 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     1801 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     1861 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     1921 tgtaaaacga cggccagtgc caagttggga tctttgcatt ggcccacggc tctcaggatg
     1981 gggatgctcc ccttcagcac ccggttcccc ttggaaactg atggtcctgg ctctgtggca
     2041 tggcagtggc actgtgagga gccccctacc agcagcacac agtggggttg gcactgccac
     2101 gctccggatg ccgcgctctg atccaacccc ataatcaagg gaacccgaat tgccccatca
     2161 ttgcccccac cacccccatc ctgccgggcc ctcacacccc acgctgcctt ctggtgacat
     2221 tccccagccc aaacccacgg cttcatggct accgcggggc atttcccatt gccgccccat
     2281 tatcagctct gcacacctcc cgctgtaccc atgcctcgtg gctgcccttc tttgacgtat
     2341 aatcttctaa ttaatacccg gccttgtcaa agtggagcac aaacgttaat taattcccca
     2401 gcaggcaggt aattaacagt gtgactccct ttttgctgcg agtggggctg atacagagag
     2461 atgtggcact atggagccca cggggtcctg gcactgggtg cccacggagg tccccatgtg
     2521 ctgcagtgtc accgcctccg aggagacagt attgtccctg cggtgtccct gcagctcagc
     2581 tctgtccaca gggccacctc cagtttggag gggacacaat gcagccccga tgcaacccat
     2641 cctcgcagca tcccagggac aaagacccca ctgcaagacc gcacacaggg ctgggtcccg
     2701 ctcccctaat atctacagtg cttcttgcat ggccccttaa tcaatgcagt taatcagcat
     2761 gcgctcatgc accgctctgg agctgcaaag cccctcgcag cgctgctcac caacaccgcg
     2821 caccgccccg gcccagcctg cagcacgcgc tgcaaacagg aaagaaacaa aatattgccc
     2881 aaatgtaggc aaaggcattc ggctgccttg acctccgccg ggccgggccc tgcctgactc
     2941 agctccttac tcagcgctcg cttcctccct ccggctgcca ccgccgcagc gcacaccctg
     3001 acaaagagtg gcccttaacg ggctctgagg tgcacccagc agtgcactca gcagtccaag
     3061 ggccggcctg gaggtttgca ccgctacgtg ctgacattag cattgaactt ggccctgggt
     3121 agtgctgcag gccgggcggg gtgggtgtag agagtgcagc gcgcgttgca cccggtgccc
     3181 cttcccctcc cttgcatccc agcaggctgc accccagcac caggcccgtg catgcatgct
     3241 cctggtgtta ttgcagcctg gtgcatgcat gcgtcttgat ggtgcagcgc tgtgcatgca
     3301 tcctccttgg tgtgtagcag tctagtgcat gcatacccct cggtgttatt gctgctctgt
     3361 gcacgcacgc tcattgtatc acttcatccc agtgcatgca ctcacactgg agcgattgct
     3421 gctcggtgca cgcacactca ttgtatcact gcagctcagt ggctgcacgc acaccggtgt
     3481 tattgctgct cggtgcgtgc atgcacatca gtgtcgctgc agctcagtgc atgcacgctc
     3541 attgcccatc gctatccctg cctctcctgc tggcgctccc cgggaggtga cttcaagggg
     3601 accgcaggac cacctcgggg gtggggggag ggctgcacac gcggaccccg ctccccctcc
     3661 ccaacaaagc actgtggaat caaaaagggg gggggggggg tggggggggg gggcgcaacc
     3721 ccgccccacc cccccccccc ggggggcgcc ccacggtctg cttcacccgt cccatgcccc
     3781 cccccccccc ccccccccca ccccctcccg gccgtagccc ccccactccc cctacccccc
     3841 cccccctccc cccccccccc cccccccatt tggttgttgt tgttttttta agtattgtgg
     3901 gcggggaggg gggggggggg gggggggcgc gcgccagggg gggcggggcg gggcgagggg
     3961 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4021 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     4081 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     4141 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     4201 cctccgggct gtaattagcg cttggtttaa tgacggctcg tttcttttct gtggctgcgt
     4261 gaaagcctta aagggctccg ggagggccct ttgtgcgggg gggagcggct cggggggtgc
     4321 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     4381 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     4441 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     4501 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt aacccccccc
     4561 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg
     4621 ggcgtggcgc ggggctcgcc gggccggggg ggggggggcg gcgggggggg gggggggggg
     4681 gggggggggg gggggggggg gggggggggg gggggggggg gggggcgggg ggcggccggg
     4741 gggggggggg gggggggggg gggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     4801 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     4861 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     4921 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     4981 gtcgccgcgc cgccgtcccc ttctccatct ccagcctcgg ggctgtccgc agggggacgg
     5041 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggggt
     5101 ttatatcttc ccttctctgt tcctccgcag cccccaagct tggcgcgcct ataatgccaa
     5161 ctttgtacaa aaaagttggc accatgtcca gcgctcggac ccccctaccc acgctgaacg
     5221 agagggacac ggagcagccc accttgggac accttgactc caagcccagc agtaagtcca
     5281 acatgattcg gggccgcaac tcagccacct ctgctgatga gcagccccac attggaaact
     5341 accggctcct caagaccatt ggcaagggta attttgccaa ggtgaagttg gcccgacaca
     5401 tcctgactgg gaaagaggta gctgtgaaga tcattgacaa gactcaactg aactcctcca
     5461 gcctccagaa actattccgc gaagtaagaa taatgaaggt tttgaatcat cccaacatag
     5521 ttaaattatt tgaagtgatt gagactgaga aaacgctcta ccttgtcatg gagtacgcta
     5581 gtggcggaga ggtatttgat tacctagtgg ctcatggcag gatgaaagaa aaagaggctc
     5641 gagccaaatt ccgccagata gtgtctgctg tgcagtactg tcaccagaag tttattgtcc
     5701 atagagactt aaaggcagaa aacctgctct tggatgctga tatgaacatc aagattgcag
     5761 actttggctt cagcaatgaa ttcacctttg ggaacaagct ggacaccttc tgtggcagtc
     5821 ccccttatgc tgccccagaa ctcttccagg gcaaaaaata tgatggaccc gaggtggatg
     5881 tgtggagcct aggagttatc ctctatacac tggtcagcgg atccctgcct tttgatggac
     5941 agaacctcaa ggagctgcgg gaacgggtac tgaggggaaa ataccgtatt ccattctaca
     6001 tgtccacgga ctgtgaaaac ctgcttaaga aatttctcat tcttaatccc agcaagagag
     6061 gcactttaga gcaaatcatg aaagatcgat ggatgaatgt gggtcacgaa gatgatgaac
     6121 taaagcctta cgtggagcca ctccctgact acaaggaccc ccggcggaca gagctgatgg
     6181 tgtccatggg ttatacacgg gaagagatcc aggactcgct ggtgggccag agatacaacg
     6241 aggtgatggc cacctatctg ctcctgggct acaagagctc cgagctggaa ggcgacacca
     6301 tcaccctgaa accccggcct tcagctgatc tgaccaatag cagcgcccca tccccatccc
     6361 acaaggtaca gcgcagcgtg tcggccaatc ccaagcagcg gcgcttcagc gaccaggctg
     6421 gtcctgccat tcccacctct aattcttact ctaagaagac tcagagtaac aacgcagaaa
     6481 ataagcggcc tgaggaggac cgggagtcag ggcggaaagc cagcagcaca gccaaggtgc
     6541 ctgccagccc cctgcccggt ctggagagga agaagaccac cccaaccccc tccacgaaca
     6601 gcgtcctctc caccagcaca aatcgaagca ggaattcccc acttttggag cgggccagcc
     6661 tcggccaggc ctccatccag aatggcaaag acagcacagc cccccagcgt gtccctgttg
     6721 cctccccatc cgcccacaac atcagcagca gtggtggagc cccagaccga actaacttcc
     6781 cccggggtgt gtccagccga agcaccttcc atgctgggca gctccgacag gtgcgggacc
     6841 agcagaattt gccctacggt gtgaccccag cctctccctc tggccacagc cagggccggc
     6901 ggggggcctc tgggagcatc ttcagcaagt tcacctccaa gtttgtacgc aggaacctga
     6961 atgaacctga aagcaaagac cgagtggaga cgctcagacc tcacgtggtg ggcagtggcg
     7021 gcaacgacaa agaaaaggaa gaatttcggg aggccaagcc ccgctccctc cgcttcacgt
     7081 ggagtatgaa gaccacgagc tccatggagc ccaacgagat gatgcgggag atccgcaagg
     7141 tgctggacgc gaacagctgc cagagcgagc tgcatgagaa gtacatgctg ctgtgcatgc
     7201 acggcacgcc gggccacgag gacttcgtgc agtgggagat ggaggtgtgc aaactgccgc
     7261 ggctctctct caacggggtt cgatttaagc ggatatcggg cacctccatg gccttcaaaa
     7321 acattgcctc caaaatagcc aacgagctga agcttggcgc gcctagggga ggaggttccg
     7381 gaggcggctc tggcggaggg gctagcggag gatccgaaat cggtactggc tttccattcg
     7441 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     7501 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     7561 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     7621 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     7681 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     7741 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     7801 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     7861 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     7921 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     7981 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     8041 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     8101 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     8161 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     8221 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     8281 ctactctgga gatttccggg ctgtacaagc ggaccgtcta atctagataa gtaatgatca
     8341 taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     8401 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     8461 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8521 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccccg
     8581 ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     8641 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8701 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8761 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8821 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agccc
//
