LOCUS       sequence-446055-        7543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Pex3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Mendoza CS, Plowinske CR, Montgomery AC, Quinones GB, Banker G, 
            Bentley M
  TITLE     Kinesin Regulation in the Proximal Axon is Essential for 
            Dendrite-selective Transport.
  JOURNAL   Mol Biol Cell. 2024 Jun 1;35(6):ar81. doi: 10.1091/mbc.E23-11-0457. 
            Epub 2024 Apr 10.
  PUBMED    38598291
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-06-1"; volume: "35(6):ar81. doi"; pages: " 
            10.1091/mbc.E23-11-0457. Epub 2024 Apr 10"
COMMENT     Sequence Label: pBa.PEX 1-42-tdTM-FKBP
FEATURES             Location/Qualifiers
     source          1..7543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     503..525
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             736..2163
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             2230..2550
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    2670..2804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2707..2726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2761..2780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2835..2851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2835..2851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2848..2870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2859..2875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2883..2889,2890..2907,2908..2913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2928..2949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3066..3083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3237..3825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3317..3336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3996..4787,4788..4856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4619..4638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4857..4961)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5029..5047
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5085..5107)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5207..5226
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5420..5442
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5434..5451
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5435..5451
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtgggggggg cgggcggggc ggccccaccc cccccgcccc cccccccccc cggggtgggg
       61 ggggcgcgcg gggggggggg gggggggcgg gggggggggg ggggcggggg gggggcgggg
      121 gggggggggg gggggggggg gggggggggg cggggggggg gggggccgcc tcgggccggg
      181 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
      241 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
      301 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
      361 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
      421 cgccgccgtc cccttctcca tctccagcct cggggctgtc cgcaggggga cggctgcctt
      481 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ggtttatatc
      541 ttcccttctc tgttcctccg cagcccccaa gcttggcgcg ccaccgccac catgctgaga
      601 tcaatgtgga attttctgaa acgtcacaaa aagaaatgca tcttcctggg cacagtcctc
      661 ggaggagtat atatcctggg aaaatatgga cagaaaaaaa ttagagaaat acaagaagcc
      721 gatcctaggc ctaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc
      781 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
      841 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      901 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
      961 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     1021 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     1081 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     1141 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     1201 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccgctacctg
     1261 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     1321 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1381 gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc
     1441 ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg
     1501 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     1561 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     1621 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     1681 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     1741 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     1801 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     1861 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     1921 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccgc
     1981 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     2041 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     2101 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac
     2161 aagcggaccg gcgctagcgg aggcggatct ggaggcggga gcggaggcgg ttccggaggc
     2221 gggactagag gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     2281 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     2341 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     2401 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     2461 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     2521 gtcttcgatg tggagcttct aaaactggaa actagttatt gaggatcata atcagccata
     2581 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     2641 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2701 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2761 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc
     2821 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2881 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2941 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3001 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3061 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4981 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5041 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5101 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5161 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5221 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5281 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     5341 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     5401 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagttg
     5461 ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag cacccggttc
     5521 cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga ggagccccct
     5581 accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct ctgatccaac
     5641 cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc atcctgccgg
     5701 gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca cggcttcatg
     5761 gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc tcccgctgta
     5821 cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac ccggccttgt
     5881 caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac agtgtgactc
     5941 cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc ccacggggtc
     6001 ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct ccgaggagac
     6061 agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac ctccagtttg
     6121 gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg gacaaagacc
     6181 ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca gtgcttcttg
     6241 catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc tggagctgca
     6301 aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg
     6361 cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca ttcggctgcc
     6421 ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc tcgcttcctc
     6481 cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta acgggctctg
     6541 aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt gcaccgctac
     6601 gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc ggggtgggtg
     6661 tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat cccagcaggc
     6721 tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg
     6781 catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag cagtctagtg
     6841 catgcatacc cctcggtgtt attgctgctc tgtgcacgca cgctcattgt atcacttcat
     6901 cccagtgcat gcactcacac tggagcgatt gctgctcggt gcacgcacac tcattgtatc
     6961 actgcagctc agtggctgca cgcacaccgg tgttattgct gctcggtgcg tgcatgcaca
     7021 tcagtgtcgc tgcagctcag tgcatgcacg ctcattgccc atcgctatcc ctgcctctcc
     7081 tgctggcgct ccccgggagg tgacttcaag gggaccgcag gaccacctcg ggggtggggg
     7141 gagggctgca cacgcggacc ccgctccccc tccccaacaa agcactgtgg aatcaaaaag
     7201 gggggagggg ggatggaggg gcgcgtcaca cccccgcccc acaccctcac ctcgaggtga
     7261 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     7321 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgccg
     7381 ggcggggggg gggggggggg gggggggggg gggggggggg gggggcggcg gggggggggg
     7441 cgggcggggg gggggggggg cgcggggggg gggtgggggg ggggtggggg gggggggggg
     7501 gggggtgggg cgaggggggg gggggggggg gggggggggg ggg
//
