LOCUS       sequence-446059-       11955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of KIF 13a S1371A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11955)
  AUTHORS   Mendoza CS, Plowinske CR, Montgomery AC, Quinones GB, Banker G, 
            Bentley M
  TITLE     Kinesin Regulation in the Proximal Axon is Essential for 
            Dendrite-selective Transport.
  JOURNAL   Mol Biol Cell. 2024 Jun 1;35(6):ar81. doi: 10.1091/mbc.E23-11-0457. 
            Epub 2024 Apr 10.
  PUBMED    38598291
REFERENCE   2  (bases 1 to 11955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-06-1"; volume: "35(6):ar81. doi"; pages: " 
            10.1091/mbc.E23-11-0457. Epub 2024 Apr 10"
COMMENT     Sequence Label: pBa.GFP-KIF13AS1371A
FEATURES             Location/Qualifiers
     source          1..11955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1630..1652
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1720..1722,1723..1725,1726..2433)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1765..1786)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2026..2045)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2373..2394
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      6211..6220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7831..7965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7868..7887)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7922..7941
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7996..8012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7996..8012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8009..8031)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8020..8036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8044..8050,8051..8068,8069..8074))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8089..8110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8227..8244)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8398..8986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8478..8497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9157..9948,9949..10017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9780..9799
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10018..10122)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10190..10208
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10246..10268)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10368..10387
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10581..10603
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10595..10612
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10596..10612
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtgcagcgct gtgcatgcat cctccttggt gtgtagcagt ctagtgcatg catacccctc
       61 ggtgttattg ctgctctgtg cacgcacgct cattgtatca cttcatccca gtgcatgcac
      121 tcacactgga gcgattgctg ctcggtgcac gcacactcat tgtatcactg cagctcagtg
      181 gctgcacgca caccggtgtt attgctgctc ggtgcgtgca tgcacatcag tgtcgctgca
      241 gctcagtgca tgcacgctca ttgcccatcg ctatccctgc ctctcctgct ggcgctcccc
      301 gggaggtgac ttcaagggga ccgcaggacc acctcggggg tggggggagg gctgcacacg
      361 cggaccccgc tccccctccc caacaaagca ctgtggaatc aaaaaggggg gaggggggtg
      421 ggaggggggg gtgcaacccg cgccccccac acttccctcc aggggggcgc cacgtcttgg
      481 ggtctccccc cccccctccc cccccccccc ccccctcccc cccccccccc ccccccactt
      541 ggttatattt ttttttttta atttttgtgg gcgccggggg gcgggggggg gggggggggc
      601 gcgccccggg gggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      661 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      721 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
      781 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      841 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      901 aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc cgggagggcc
      961 ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
     1021 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1081 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1141 gggtgcgggg gggacagagg gtgcgggggg ggggcggggg gggggggggg gggggggggg
     1201 gggggggggg gggggggggg gggggggggg cgcgggggcc ggcggggcgg gggggggggg
     1261 gggggggggg gggggggggg gggggggcgg ggcggcgggg gggcgggggg gggcgggggg
     1321 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     1381 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     1441 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     1501 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     1561 ttctccatct ccagcctcgg ggctgtccgc agggggacgg ctgccttcgg gggggacggg
     1621 gcagggcggg gttcggcttc tggcgtgtga ccggcggggt ttatatcttc ccttctctgt
     1681 tcctccgcag cccccaagct tggcgcgcct aggcctacca tggtgagcaa gggcggggag
     1741 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1801 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1861 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1921 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1981 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2041 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2101 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2161 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2221 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2281 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2341 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2401 gccgggatca ctctcggcat ggacgagctg tacctgacgc gtggaggagc tggaataggc
     2461 atgtcggata cgaaggtaaa agttgccgtc cgggtccggc ccatgaaccg aagagagctg
     2521 gaactgaaca ccaagtgcgt ggtggagatg gaagggaacc agacggtcct gcaccctcct
     2581 ccttccaaca ctaagcaggg agaaagaaaa cctcccaagg tgtttgcctt tgattattgc
     2641 ttttggtcca tggatgaatc gaacacaaca aaatatgctg gtcaagaagt cgttttcaag
     2701 tgcctcgggg aaggaattct cgagaaagcc tttcagggat acaatgcttg tatttttgca
     2761 tatggccaga caggttctgg aaagtccttt tccatgatgg gccacgcaga gcagctgggc
     2821 cttatcccaa ggctctgctg tgctctgttt caaaggatcg ccctggagca aaatgagtca
     2881 cagaccttca aagtcgaggt atcgtatatg gagatctata acgagaaggt ccgggacctc
     2941 ttggacccca aagggagcag gcagtctctg aaagtccgag agcataaggt cctggggccg
     3001 tatgtcgatg gtctgtctca gctggctgtc accagctttg aggacattga gtcactgatg
     3061 tctgagggaa ataagtctcg aacggtagca gcgaccaaca tgaatgagga gagcagccgt
     3121 tcccatgcgg tcttcaacat catcatcaca cagacgctgt atgacctaca gtctgggaac
     3181 tccggggaga aggtcagcaa ggtgagcctg gtagacctgg cggggagcga gagagtgtcg
     3241 aagactggag ctgcgggtga gcggctgaaa gaaggcagca acattaacaa atcccttaca
     3301 accttgggat tggttatatc gtcactggct gaccaggctg ctggcaaagg caaaaacaaa
     3361 ttcgtgcctt atcgagattc ggtccttact tggcttctta aggacaattt aggagggaac
     3421 agccagacct ctatgatagc gaccatcagc ccagcagcag acaactatga ggagacactg
     3481 tccacgctaa gatatgcaga tcgagccaag aggattgtga accacgctgt ggtgaatgag
     3541 gatcccaatg ccaaggtcat ccgagagctt cgagaggaag tggagaaact gagggagcag
     3601 ctctcgcagg ctgaggccat gaaagccccc gagctaaagg agaagctcga agagtctgag
     3661 aagctgataa aggagctaac ggtgacgtgg gaagagaagc tgaggaaaac agaagcgatc
     3721 gcacaggaga gacagcggca gctggaaagc atgggaatct ccctggagac ctctggcatc
     3781 aaggtggggg acgacaagtg ctacctcgtc aacctgaacg cagaccctgc cctcaacgag
     3841 cttctggttt actatttaaa ggatcatacc cgggtgggtg cagatacctc tcaggacatc
     3901 cagctcttcg gaatagggat tcagcccgag cactgtgaga tcgacatcgc agcggatgga
     3961 gacatcactc tcacgcccaa agaaaatgca aggtcctgtg gcaatggcac ccttgtctgc
     4021 agtactaccc agctgtggca cggcgacagg atcttatggg gaaacaacca cttttttaga
     4081 attaacttgc ctaagaggaa acggcgggac tggttgaaag actttgagag agaaaccagt
     4141 tccgcggagc acgatctaga cgctgcgagc gaggcttcct cggagccaga ctacaactac
     4201 gagttcgcac agatggaggt catcatgaaa acactgaaca gcaatgatcc agttcaaaat
     4261 gtggttcagg tcctggagaa gcagtacctg gaagagaagc ggaccgccct ggaggaacag
     4321 cggctcatgt acgaacgcga gctggagcag cttcgccagc agctctctcc cgagaggcag
     4381 ccgcccagca gcgcctcaga ccgtctggcc tacagcagcc agacagccca gcagaaggtg
     4441 acccagtggg ccgaggagag ggatgaactc tttaggcaga gcttggccaa gcttcgagag
     4501 cagctggtga aagccaacac tttggtgagg gaagccaact tcctagccga ggagatgagc
     4561 aaactcactg actaccaagt gactctgcag atccctgcgg ccaacctcag tgccaacagg
     4621 aagagaggcg caatcgtcag cgagcccgcc atccaagcca ggaggaaagg gaagggcacc
     4681 caggtctgga ccattgagaa gctggagaat aagctaattg atatgagaga cctttaccaa
     4741 gaatggaaag aaaatgttcc tgaggccaag aggctctatg ggaagcgagg tgaccctttt
     4801 tatgaagcgc aggagaatca caacctcatt ggtgtggcta atgtgttttt ggaatgtctc
     4861 ttctgtgatg tgaagcttca atatgcagta cccatcatca gccagcaggg ggaggttgcc
     4921 gggcgcctcc atgtggaggt gacgcgcatt acaggaacca ttccagaacg catggcggag
     4981 gatgactctt cagagaactc tagtgagagc gggagtcttg aagtggtcga cagcagcggc
     5041 gaagtcattc accgggtcaa aaagctgaca tgcagggtga taattaaaga ggcaaccggg
     5101 ctacccataa gcctctcaaa tttcgtcttc tgtcagtaca cgttctggga ccagtgtgag
     5161 tctacagtgg ctgctccagt ggtggaccca gatgtgccct ccccgcagtc caaggacgca
     5221 cagtacacgg tgaccttctc tcactgtaag gactacgtgg tgactgtcac agaagagttc
     5281 ctggagttca tttctgatgg tgcgctggcg attgaagtgt ggggccacag gtgtgctggg
     5341 aacggaagcc ccatctggga ggtggactcg ctccatgcga agacaaggac attgcatgac
     5401 aggtggaatg aagtgacccg gagaatagag gtgtggatct ccattctgga gctgaatgag
     5461 ctcggggatt atgccgctgt ggagcttcac caggcgaaag acgtcaacac cggcggcgtc
     5521 ttccaactca gacagggtca ctcccgtaga gtgcaggtca cagtgaaacc tgtacagcac
     5581 tcagggacac tgccactcat ggttgaagcc atcctatcag tatccattgg ctgcgtgacc
     5641 gccaggtcca ccaagctgca gagagggctg gacagctacc aggaagaaga cttgaattgt
     5701 gtaagagaga gatggtccga tgcactcatt aaacgacggg aatacttaga cgaacagatt
     5761 aaaaaagtca gcaataaaaa agagaaaaca gaagacgaca tggagcggga agccaggctg
     5821 gtggagcagt gggtggggct gacagaggag aggaatgctg tgctggtgcc cgcccctggc
     5881 agcggaatcc ccggggcacc tgctgactgg gtcccacctc ctggcatgga aacacacatc
     5941 ccggttctct tcctggatct gaacgcggat gacctcagtg ccaatgagca gctggtgggc
     6001 ccccatgcgt ccggtgtgaa ctccatcctg cccaaggaac acggcagtca gtttttctac
     6061 ctgcccatca taaagcacag tgatgacgag gtttcagcca cagcctcctg ggactcctct
     6121 gtgcacgact ctcttcacct gaatcgggtc acaccgcaga atgaaaggat ctacctgatc
     6181 gtgaagacca ccgtgcagct cagccacccg gccgccatgg agctagtgtt gcggaagcgg
     6241 atcgcggcca atatttataa caagcagagt ttcacccaga gtttgaagag acgaatatca
     6301 ctgatcaata tacgttactc ctgcggcgtg acctatgaga tagtatccaa tatcccaaag
     6361 gcgaccgagg agattgagga ccgggaaacc ctggccctcc tggcagcgcg gagtgagaac
     6421 gagggcactt tggatgggga gacatacatc gagaagtaca cgcggggcgt gctgcaggtg
     6481 gagaatatcc tgagcctgga gcggctccgg caggctgtca cggtgaagga agcgctttcc
     6541 accaaagcga ggcacattcg gagaagcctc gcgacaccga atgttcacaa cgtctcctct
     6601 agccggccag atctctctgg cttcgatgaa gacgataagg gctggcccga gaaccagtta
     6661 gacgtgtctg actacagctc cagctaccaa gacgtcgcgt gctatgggac tttacccagg
     6721 gactcacctc gcaggagcaa ggaaggttgt ccatcagaga atcctcatgc cttaacagtc
     6781 agccctttta aagcgttctc tcctcagcca ccaaagttct tcaaacccct catgcctgta
     6841 aaagaggagc ataagaaaag gttggccctg gaagccagac ctctcctaag ccaagaggac
     6901 tcggaagagg aggaaaacga gctggaagct ctcagcagga agctgatgct tacacagcct
     6961 tatgtgcccg tggagttcgc tgacttcagt gtttacaatg ccagcttgga gaacagggag
     7021 tggtcctctt ctaaggcgga cctgacagac tcgagagcct tggagaaagc agtgtcccgt
     7081 agccctacca ccagcagcct caccagtggc tacttctccc acagtgcctc caatgccact
     7141 ctgtctgaca tggcagtccc ctccagcgac agctcagacc agcttgccgt ctcgacaaag
     7201 gaggtcgagt gcgccgagcc cccgggacca tcacttgcac cggatgtcag acgagcttcg
     7261 aatcaggagc ttacagaagt aggaagaggc tcagggaagg atgagacaat cgctgtgccg
     7321 ctggaggaaa acagtgcctt acccaagggg accccgtcac cccagagcat ccctgaggaa
     7381 agttccagaa tgccatgcag gactgcttca tgttcagaac tggatgtcgg ccccagcaaa
     7441 gatggccacc aagccagaga attctgccca ggagaggtga cgatagaaca cactacaaac
     7501 attttggagg accattcctt cacagagttc atgggtgttt ctgatggcaa agactttgat
     7561 ggtttggcag attgttctgt aggggagccc tccaggagga gggctctaac aaacgaaaca
     7621 gaccataaag gtatcccaga aagacctcca gatgccgacc ggctgcatcc caagatagag
     7681 aacgaccagg aagccacagc cactcggtaa gctagataag taatgatcat aatcagccat
     7741 atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     7801 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     7861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7921 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccccgg gtaccgagct
     7981 cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8161 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8221 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8821 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9061 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9121 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9181 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9241 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9301 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9361 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9421 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9481 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9541 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9601 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9661 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9721 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9781 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9841 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9901 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9961 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10021 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10081 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10141 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10201 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10261 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10321 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10381 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10441 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10501 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10561 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagtt
    10621 gggatctttg cattggccca cggctctcag gatggggatg ctccccttca gcacccggtt
    10681 ccccttggaa actgatggtc ctggctctgt ggcatggcag tggcactgtg aggagccccc
    10741 taccagcagc acacagtggg gttggcactg ccacgctccg gatgccgcgc tctgatccaa
    10801 ccccataatc aagggaaccc gaattgcccc atcattgccc ccaccacccc catcctgccg
    10861 ggccctcaca ccccacgctg ccttctggtg acattcccca gcccaaaccc acggcttcat
    10921 ggctaccgcg gggcatttcc cattgccgcc ccattatcag ctctgcacac ctcccgctgt
    10981 acccatgcct cgtggctgcc cttctttgac gtataatctt ctaattaata cccggccttg
    11041 tcaaagtgga gcacaaacgt taattaattc cccagcaggc aggtaattaa cagtgtgact
    11101 ccctttttgc tgcgagtggg gctgatacag agagatgtgg cactatggag cccacggggt
    11161 cctggcactg ggtgcccacg gaggtcccca tgtgctgcag tgtcaccgcc tccgaggaga
    11221 cagtattgtc cctgcggtgt ccctgcagct cagctctgtc cacagggcca cctccagttt
    11281 ggaggggaca caatgcagcc ccgatgcaac ccatcctcgc agcatcccag ggacaaagac
    11341 cccactgcaa gaccgcacac agggctgggt cccgctcccc taatatctac agtgcttctt
    11401 gcatggcccc ttaatcaatg cagttaatca gcatgcgctc atgcaccgct ctggagctgc
    11461 aaagcccctc gcagcgctgc tcaccaacac cgcgcaccgc cccggcccag cctgcagcac
    11521 gcgctgcaaa caggaaagaa acaaaatatt gcccaaatgt aggcaaaggc attcggctgc
    11581 cttgacctcc gccgggccgg gccctgcctg actcagctcc ttactcagcg ctcgcttcct
    11641 ccctccggct gccaccgccg cagcgcacac cctgacaaag agtggccctt aacgggctct
    11701 gaggtgcacc cagcagtgca ctcagcagtc caagggccgg cctggaggtt tgcaccgcta
    11761 cgtgctgaca ttagcattga acttggccct gggtagtgct gcaggccggg cggggtgggt
    11821 gtagagagtg cagcgcgcgt tgcacccggt gccccttccc ctcccttgca tcccagcagg
    11881 ctgcacccca gcaccaggcc cgtgcatgca tgctcctggt gttattgcag cctggtgcat
    11941 gcatgcgtct tgatg
//
